Phospatidylserine or ganglioside : which of anionic lipids determines the effect of cationic dextran on lipid membrane?

2015
journal article
article
cris.lastimport.wos2024-04-09T23:30:27Z
dc.abstract.enIn this work the influence of cationic polymer, namely diethylaminoethyl DEAE-dextran on model lipid membranes was investigated. This polymer is of a wide application as a biomaterial and a drug carrier and its cytotoxicity toward various cancer cells was also confirmed. It was suggested that anticancer effect of cationic dextran is connected with the binding of the polymer to the negatively charged sialic acid residues overexpressed in cancer membrane. This fact encouraged us to perform the studies aimed at verifying whether the effect of cationic DEAE-dextran on membrane is determined only by the presence of the negatively charged lipid in the system or the kind of anionic lipid is also important. To reach this goal systematic investigations on the effect of dextran on various one-component lipid monolayers and multicomponent hepatoma cell model membranes differing in the level and the kind of anionic lipids (phosphatidylserine, sialic acid-containing ganglioside GM3 or their mixture) were done. As evidenced the results the effect of DEAE-dextran on the model system is determined by anionic lipid–polymer electrostatic interactions. However, the magnitude of the effect of cationic polymer is strongly dependent on the kind of anionic lipid in the model system. Namely, the packing and ordering of the mixtures containing ganglioside GM3 were more affected by DEAE-dextran than phosphatidylserine-containing monolayers. Although the experiments were done on model systems and therefore further studies are highly needed, the collected data may indicate that ganglioside may be important in the differentiation of the effect of cationic dextran on membranes.pl
dc.affiliationWydział Chemii : Zakład Chemii Fizycznej i Elektrochemiipl
dc.affiliationWydział Chemii : Zakład Chemii Środowiskapl
dc.contributor.authorHąc-Wydro, Katarzyna - 128297 pl
dc.contributor.authorWydro, Paweł - 132797 pl
dc.contributor.authorWłodarczyk, Grzegorzpl
dc.contributor.authorCetnar, Andrzejpl
dc.date.accessioned2015-07-02T09:27:37Z
dc.date.available2015-07-02T09:27:37Z
dc.date.issued2015pl
dc.description.admin[AB] Cetnar, Andrzej 50000141pl
dc.description.admin[AB] Włodarczyk, Grzegorz 50000141pl
dc.description.physical204-209pl
dc.description.volume126pl
dc.identifier.doi10.1016/j.colsurfb.2014.12.031pl
dc.identifier.eissn1873-4367pl
dc.identifier.issn0927-7765pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/11175
dc.languageengpl
dc.language.containerengpl
dc.rights.licencebez licencji
dc.subtypeArticlepl
dc.titlePhospatidylserine or ganglioside : which of anionic lipids determines the effect of cationic dextran on lipid membrane?pl
dc.title.journalColloids and Surfaces. B, Biointerfacespl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T23:30:27Z
dc.abstract.enpl
In this work the influence of cationic polymer, namely diethylaminoethyl DEAE-dextran on model lipid membranes was investigated. This polymer is of a wide application as a biomaterial and a drug carrier and its cytotoxicity toward various cancer cells was also confirmed. It was suggested that anticancer effect of cationic dextran is connected with the binding of the polymer to the negatively charged sialic acid residues overexpressed in cancer membrane. This fact encouraged us to perform the studies aimed at verifying whether the effect of cationic DEAE-dextran on membrane is determined only by the presence of the negatively charged lipid in the system or the kind of anionic lipid is also important. To reach this goal systematic investigations on the effect of dextran on various one-component lipid monolayers and multicomponent hepatoma cell model membranes differing in the level and the kind of anionic lipids (phosphatidylserine, sialic acid-containing ganglioside GM3 or their mixture) were done. As evidenced the results the effect of DEAE-dextran on the model system is determined by anionic lipid–polymer electrostatic interactions. However, the magnitude of the effect of cationic polymer is strongly dependent on the kind of anionic lipid in the model system. Namely, the packing and ordering of the mixtures containing ganglioside GM3 were more affected by DEAE-dextran than phosphatidylserine-containing monolayers. Although the experiments were done on model systems and therefore further studies are highly needed, the collected data may indicate that ganglioside may be important in the differentiation of the effect of cationic dextran on membranes.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii
dc.affiliationpl
Wydział Chemii : Zakład Chemii Środowiska
dc.contributor.authorpl
Hąc-Wydro, Katarzyna - 128297
dc.contributor.authorpl
Wydro, Paweł - 132797
dc.contributor.authorpl
Włodarczyk, Grzegorz
dc.contributor.authorpl
Cetnar, Andrzej
dc.date.accessioned
2015-07-02T09:27:37Z
dc.date.available
2015-07-02T09:27:37Z
dc.date.issuedpl
2015
dc.description.adminpl
[AB] Cetnar, Andrzej 50000141
dc.description.adminpl
[AB] Włodarczyk, Grzegorz 50000141
dc.description.physicalpl
204-209
dc.description.volumepl
126
dc.identifier.doipl
10.1016/j.colsurfb.2014.12.031
dc.identifier.eissnpl
1873-4367
dc.identifier.issnpl
0927-7765
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/11175
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights.licence
bez licencji
dc.subtypepl
Article
dc.titlepl
Phospatidylserine or ganglioside : which of anionic lipids determines the effect of cationic dextran on lipid membrane?
dc.title.journalpl
Colloids and Surfaces. B, Biointerfaces
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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