Fear learning increases the number of polyribosomes associated with excitatory and inhibitory synapses in the barrel cortex

2013
journal article
article
dc.abstract.enAssociative fear learning, resulting from whisker stimulation paired with application of a mild electric shock to the tail in a classical conditioning paradigm, changes the motor behavior of mice and modifies the cortical functional representation of sensory receptors involved in the conditioning. It also induces the formation of new inhibitory synapses on double-synapse spines of the cognate barrel hollows. We studied density and distribution of polyribosomes, the putative structural markers of enhanced synaptic activation, following conditioning. By analyzing serial sections of the barrel cortex by electron microscopy and stereology, we found that the density of polyribosomes was significantly increased in dendrites of the barrel activated during conditioning. The results revealed fear learning-induced increase in the density of polyribosomes associated with both excitatory and inhibitory synapses located on dendritic spines (in both single- and double-synapse spines) and only with the inhibitory synapses located on dendritic shafts. This effect was accompanied by a significant increase in the postsynaptic density area of the excitatory synapses on single-synapse spines and of the inhibitory synapses on double-synapse spines containing polyribosomes. The present results show that associative fear learning not only induces inhibitory synaptogenesis, as demonstrated in the previous studies, but also stimulates local protein synthesis and produces modifications of the synapses that indicate their potentiation.pl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.affiliationWydział Lekarski : Zakład Histologiipl
dc.cm.id58545
dc.contributor.authorJasińska, Małgorzata - 199998 pl
dc.contributor.authorSiucińska, Ewapl
dc.contributor.authorJasek, Ewapl
dc.contributor.authorLitwin, Jan - 130699 pl
dc.contributor.authorPyza, Elżbieta - 131603 pl
dc.contributor.authorKossut, Małgorzatapl
dc.date.accessioned2015-04-22T09:10:17Z
dc.date.available2015-04-22T09:10:17Z
dc.date.issued2013pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number2pl
dc.description.points40pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume8pl
dc.identifier.articleide54301pl
dc.identifier.doi10.1371/journal.pone.0054301pl
dc.identifier.eissn1932-6203pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/5451
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses*
dc.share.typeotwarte czasopismo
dc.subtypeArticlepl
dc.titleFear learning increases the number of polyribosomes associated with excitatory and inhibitory synapses in the barrel cortexpl
dc.title.journalPLoS ONEpl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Associative fear learning, resulting from whisker stimulation paired with application of a mild electric shock to the tail in a classical conditioning paradigm, changes the motor behavior of mice and modifies the cortical functional representation of sensory receptors involved in the conditioning. It also induces the formation of new inhibitory synapses on double-synapse spines of the cognate barrel hollows. We studied density and distribution of polyribosomes, the putative structural markers of enhanced synaptic activation, following conditioning. By analyzing serial sections of the barrel cortex by electron microscopy and stereology, we found that the density of polyribosomes was significantly increased in dendrites of the barrel activated during conditioning. The results revealed fear learning-induced increase in the density of polyribosomes associated with both excitatory and inhibitory synapses located on dendritic spines (in both single- and double-synapse spines) and only with the inhibitory synapses located on dendritic shafts. This effect was accompanied by a significant increase in the postsynaptic density area of the excitatory synapses on single-synapse spines and of the inhibitory synapses on double-synapse spines containing polyribosomes. The present results show that associative fear learning not only induces inhibitory synaptogenesis, as demonstrated in the previous studies, but also stimulates local protein synthesis and produces modifications of the synapses that indicate their potentiation.
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.affiliationpl
Wydział Lekarski : Zakład Histologii
dc.cm.id
58545
dc.contributor.authorpl
Jasińska, Małgorzata - 199998
dc.contributor.authorpl
Siucińska, Ewa
dc.contributor.authorpl
Jasek, Ewa
dc.contributor.authorpl
Litwin, Jan - 130699
dc.contributor.authorpl
Pyza, Elżbieta - 131603
dc.contributor.authorpl
Kossut, Małgorzata
dc.date.accessioned
2015-04-22T09:10:17Z
dc.date.available
2015-04-22T09:10:17Z
dc.date.issuedpl
2013
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
2
dc.description.pointspl
40
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
8
dc.identifier.articleidpl
e54301
dc.identifier.doipl
10.1371/journal.pone.0054301
dc.identifier.eissnpl
1932-6203
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/5451
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses
dc.share.type
otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
Fear learning increases the number of polyribosomes associated with excitatory and inhibitory synapses in the barrel cortex
dc.title.journalpl
PLoS ONE
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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