The effect of mild rehydration on freeze-dried dipalmitoylphosphatidylcholine (DPPC) multilamellar membranes as observed by proton NMR and sorption isotherm

2016
journal article
article
cris.lastimport.wos2024-04-09T21:31:50Z
dc.abstract.enBetween neighbouring bilayers of lyophilized dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles the total number of water molecules equals 9 H2O molecules/1 DPPC molecule. One of these molecules is very tightly bound to the lipid molecule, seven are in immobilized (tightly bound) water fraction whereas the last one belongs to mobile water fraction. The rehydration from the gaseous phase of the DPPC model membranes was investigated using hydration kinetics, sorption isotherm, and high power proton relaxometry. The obtained data for DPPC were compared with these obtained for wheat photosynthetic membranes. Rehydrated photosynthetic membranes differ from DPPC model membranes in hydration kinetics. The average hydration time has a similar value: (22.0 ± 2.8) h (photosynthetic membrane) and (19.8 ± 1.6) h (DPPC), however hydration kinetics was described by one-exponential function for photosynthetic membrane, while for model membrane it shows fine double exponential form. The sigmoidal form: of sorption isotherm is better fitted using Dent model than by the Brunauer-Emmett-Teller formula. The Brunauer-Emmett-Teller/Dent deviation parameter b =0.93 either for photosynthetic or for model membranes. The mass of water saturating primary water binding sites equals ΔM/m0= 0.017 (wheat photosynthetic membranes) and 0.027 (DPPC). The detected by NMR-isotherm study mass of water "sealed" in model membrane structures was about ΔMs/m0=0.182 (about 7-8 H2O molecules /1 DPPC molecule), and ΔMs/m0= 0.066 for photosynthetic membrane.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biochemii Roślinpl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.contributor.authorHarańczyk, Hubert - 101548 pl
dc.contributor.authorLeja, Annapl
dc.contributor.authorNowak, Piotr - 105359 pl
dc.contributor.authorBaran, Ewelina - 186017 pl
dc.contributor.authorStrzałka, Kazimierz - 132129 pl
dc.date.accessioned2016-04-11T06:03:22Z
dc.date.available2016-04-11T06:03:22Z
dc.date.issued2016pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number2pl
dc.description.physical179-184pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume129pl
dc.identifier.doi10.12693/APhysPolA.129.179pl
dc.identifier.eissn1898-794Xpl
dc.identifier.issn0587-4246pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/23733
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa*
dc.rights.licenceOTHER
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subtypeArticlepl
dc.titleThe effect of mild rehydration on freeze-dried dipalmitoylphosphatidylcholine (DPPC) multilamellar membranes as observed by proton NMR and sorption isothermpl
dc.title.journalActa Physica Polonica. Apl
dc.title.volumeProceedings of the 50th Zakopane School of Physics International Symposium Breaking Frontiers : Submicron Structures in Physics and Biology, Zakopane, Poland, May 18-23, 2015pl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T21:31:50Z
dc.abstract.enpl
Between neighbouring bilayers of lyophilized dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles the total number of water molecules equals 9 H2O molecules/1 DPPC molecule. One of these molecules is very tightly bound to the lipid molecule, seven are in immobilized (tightly bound) water fraction whereas the last one belongs to mobile water fraction. The rehydration from the gaseous phase of the DPPC model membranes was investigated using hydration kinetics, sorption isotherm, and high power proton relaxometry. The obtained data for DPPC were compared with these obtained for wheat photosynthetic membranes. Rehydrated photosynthetic membranes differ from DPPC model membranes in hydration kinetics. The average hydration time has a similar value: (22.0 ± 2.8) h (photosynthetic membrane) and (19.8 ± 1.6) h (DPPC), however hydration kinetics was described by one-exponential function for photosynthetic membrane, while for model membrane it shows fine double exponential form. The sigmoidal form: of sorption isotherm is better fitted using Dent model than by the Brunauer-Emmett-Teller formula. The Brunauer-Emmett-Teller/Dent deviation parameter b =0.93 either for photosynthetic or for model membranes. The mass of water saturating primary water binding sites equals ΔM/m0= 0.017 (wheat photosynthetic membranes) and 0.027 (DPPC). The detected by NMR-isotherm study mass of water "sealed" in model membrane structures was about ΔMs/m0=0.182 (about 7-8 H2O molecules /1 DPPC molecule), and ΔMs/m0= 0.066 for photosynthetic membrane.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biochemii Roślin
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
dc.contributor.authorpl
Harańczyk, Hubert - 101548
dc.contributor.authorpl
Leja, Anna
dc.contributor.authorpl
Nowak, Piotr - 105359
dc.contributor.authorpl
Baran, Ewelina - 186017
dc.contributor.authorpl
Strzałka, Kazimierz - 132129
dc.date.accessioned
2016-04-11T06:03:22Z
dc.date.available
2016-04-11T06:03:22Z
dc.date.issuedpl
2016
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
2
dc.description.physicalpl
179-184
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
129
dc.identifier.doipl
10.12693/APhysPolA.129.179
dc.identifier.eissnpl
1898-794X
dc.identifier.issnpl
0587-4246
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/23733
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa
dc.rights.licence
OTHER
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
The effect of mild rehydration on freeze-dried dipalmitoylphosphatidylcholine (DPPC) multilamellar membranes as observed by proton NMR and sorption isotherm
dc.title.journalpl
Acta Physica Polonica. A
dc.title.volumepl
Proceedings of the 50th Zakopane School of Physics International Symposium Breaking Frontiers : Submicron Structures in Physics and Biology, Zakopane, Poland, May 18-23, 2015
dc.typepl
JournalArticle
dspace.entity.type
Publication

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