Polyhedrocytes in blood clots of type 2 diabetic patients with high cardiovascular risk : association with glycemia, oxidative stress and platelet activation

2018
journal article
article
dc.abstract.enBackground: Little is known about factors that affect the composition of contracted blood clots in specific diseases. We investigated the content of polyhedral erythrocytes (polyhedrocytes) formed in blood clots and its determinants in type 2 diabetes (T2D) patients. Methods: In 97 patients with long-standing T2D [median HbA1c, 6.4% (interquartile range 5.9–7.8)], we measured in vitro the composition of blood clots, including a clot area covered by polyhedrocytes using scanning electron microscopy and the erythrocyte compression index (ECI), defined as a ratio of the mean polyhedrocyte area to the mean native erythrocyte area. Moreover, plasma fibrin clot permeability (Ks), clot lysis time (CLT), thrombin generation, oxidative stress [total protein carbonyl (total PC), total antioxidant capacity and thiobarbituric acid reactive substances (TBARS)], and platelet activation markers were determined. The impact of glucose concentration on polyhedrocytes formation was assessed in vitro. Results: Polyhedrocytes content in contracted clots was positively correlated with glucose (r = 0.24, p = 0.028), glycated hemoglobin (r = 0.40, p = 0.024), total cholesterol (r = 0.22, p = 0.044), TBARS (r = 0.60, p = 0.0027), P-selectin (r = 0.54, p = 0.0078) and platelet factor-4, PF4 (r = 0.59, p = 0.0032), but not with thrombin generation, platelet count, Ks or CLT. Patients who formed more polyhedrocytes (≥ 10th percentile) (n = 83, 85.6%) had higher glucose (+ 15.7%, p = 0.018), fibrinogen (+ 16.6%, p = 0.004), lower red blood cell distribution width (RDW, − 8.8%, p = 0.034), reduced plasma clot density (− 21.8% Ks, p = 0.011) and impaired fibrinolysis (+ 6.5% CLT, p = 0.037) when compared to patients with lesser amount of polyhedrocytes (< 10th percentile). ECI and the content of polyhedrocytes were strongly associated with total PC (r = 0.79, p = 0.036 and r = 0.67, p = 0.0004, respectively). In vitro an increase of glucose concentration by 10 mmol/L was associated with 94% higher polyhedrocytes content (p = 0.033) when compared to the baseline (7.1 mM). After adjustment for age, sex and fibrinogen, multiple regression analysis showed that RDW was the only independent predictor of polyhedrocytes content in T2D (OR = 0.61, 95% CI 0.39–0.92). Conclusions: Poor glycemic control, together with enhanced platelet activation and oxidative stress, increase the content of polyhedrocytes in blood clots generated in T2D patients.pl
dc.affiliationWydział Lekarski : Instytut Kardiologiipl
dc.cm.date2020-01-07
dc.cm.id92114
dc.contributor.authorGajos, Grzegorz - 200709 pl
dc.contributor.authorSiniarski, Aleksander - 169737 pl
dc.contributor.authorNatorska, Joanna - 130892 pl
dc.contributor.authorZąbczyk, Michał - 137370 pl
dc.contributor.authorSiudut, Jakub - 358111 pl
dc.contributor.authorMalinowski, Krzysztofpl
dc.contributor.authorGołębiowska-Wiatrak, Renata - 140934 pl
dc.contributor.authorRostoff, Paweł - 301326 pl
dc.contributor.authorUndas, Anetta - 133708 pl
dc.date.accessioned2020-01-17T10:02:47Z
dc.date.available2020-01-17T10:02:47Z
dc.date.issued2018pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.points35pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume17pl
dc.identifier.articleid146pl
dc.identifier.doi10.1186/s12933-018-0789-6pl
dc.identifier.eissn1475-2840
dc.identifier.issn1475-2840pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/144749
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.endiabetespl
dc.subject.enfibrinpl
dc.subject.enblood clotpl
dc.subject.enpolyhedrocytespl
dc.subject.enprotein carbonylationpl
dc.subtypeArticlepl
dc.titlePolyhedrocytes in blood clots of type 2 diabetic patients with high cardiovascular risk : association with glycemia, oxidative stress and platelet activationpl
dc.title.journalCardiovascular Diabetologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Background: Little is known about factors that affect the composition of contracted blood clots in specific diseases. We investigated the content of polyhedral erythrocytes (polyhedrocytes) formed in blood clots and its determinants in type 2 diabetes (T2D) patients. Methods: In 97 patients with long-standing T2D [median HbA1c, 6.4% (interquartile range 5.9–7.8)], we measured in vitro the composition of blood clots, including a clot area covered by polyhedrocytes using scanning electron microscopy and the erythrocyte compression index (ECI), defined as a ratio of the mean polyhedrocyte area to the mean native erythrocyte area. Moreover, plasma fibrin clot permeability (Ks), clot lysis time (CLT), thrombin generation, oxidative stress [total protein carbonyl (total PC), total antioxidant capacity and thiobarbituric acid reactive substances (TBARS)], and platelet activation markers were determined. The impact of glucose concentration on polyhedrocytes formation was assessed in vitro. Results: Polyhedrocytes content in contracted clots was positively correlated with glucose (r = 0.24, p = 0.028), glycated hemoglobin (r = 0.40, p = 0.024), total cholesterol (r = 0.22, p = 0.044), TBARS (r = 0.60, p = 0.0027), P-selectin (r = 0.54, p = 0.0078) and platelet factor-4, PF4 (r = 0.59, p = 0.0032), but not with thrombin generation, platelet count, Ks or CLT. Patients who formed more polyhedrocytes (≥ 10th percentile) (n = 83, 85.6%) had higher glucose (+ 15.7%, p = 0.018), fibrinogen (+ 16.6%, p = 0.004), lower red blood cell distribution width (RDW, − 8.8%, p = 0.034), reduced plasma clot density (− 21.8% Ks, p = 0.011) and impaired fibrinolysis (+ 6.5% CLT, p = 0.037) when compared to patients with lesser amount of polyhedrocytes (< 10th percentile). ECI and the content of polyhedrocytes were strongly associated with total PC (r = 0.79, p = 0.036 and r = 0.67, p = 0.0004, respectively). In vitro an increase of glucose concentration by 10 mmol/L was associated with 94% higher polyhedrocytes content (p = 0.033) when compared to the baseline (7.1 mM). After adjustment for age, sex and fibrinogen, multiple regression analysis showed that RDW was the only independent predictor of polyhedrocytes content in T2D (OR = 0.61, 95% CI 0.39–0.92). Conclusions: Poor glycemic control, together with enhanced platelet activation and oxidative stress, increase the content of polyhedrocytes in blood clots generated in T2D patients.
dc.affiliationpl
Wydział Lekarski : Instytut Kardiologii
dc.cm.date
2020-01-07
dc.cm.id
92114
dc.contributor.authorpl
Gajos, Grzegorz - 200709
dc.contributor.authorpl
Siniarski, Aleksander - 169737
dc.contributor.authorpl
Natorska, Joanna - 130892
dc.contributor.authorpl
Ząbczyk, Michał - 137370
dc.contributor.authorpl
Siudut, Jakub - 358111
dc.contributor.authorpl
Malinowski, Krzysztof
dc.contributor.authorpl
Gołębiowska-Wiatrak, Renata - 140934
dc.contributor.authorpl
Rostoff, Paweł - 301326
dc.contributor.authorpl
Undas, Anetta - 133708
dc.date.accessioned
2020-01-17T10:02:47Z
dc.date.available
2020-01-17T10:02:47Z
dc.date.issuedpl
2018
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.pointspl
35
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
17
dc.identifier.articleidpl
146
dc.identifier.doipl
10.1186/s12933-018-0789-6
dc.identifier.eissn
1475-2840
dc.identifier.issnpl
1475-2840
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/144749
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
diabetes
dc.subject.enpl
fibrin
dc.subject.enpl
blood clot
dc.subject.enpl
polyhedrocytes
dc.subject.enpl
protein carbonylation
dc.subtypepl
Article
dc.titlepl
Polyhedrocytes in blood clots of type 2 diabetic patients with high cardiovascular risk : association with glycemia, oxidative stress and platelet activation
dc.title.journalpl
Cardiovascular Diabetology
dc.typepl
JournalArticle
dspace.entity.type
Publication

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