Glial expression of DmMANF is required for the regulation of activity, sleep and circadian rhythms in the visual system of Drosophila melanogaster

2021
journal article
article
4
cris.lastimport.wos2024-04-10T00:14:34Z
dc.abstract.enDmMANF, Drosophila melanogaster mesencephalic astrocyte-derived neurotrophic factor (DmMANF) is an evolutionarily conserved orthologue of mammalian MANF. This neurotrophic factor exerts many functions in the Drosophila brain, particularly those dependent on glial cells. As we found in our earlier study, downregulation of DmMANF in glia induces degeneration of glial cells in the first optic neuropil (lamina) where DmMANF abundance is especially high. In the present study, we observed that changes in the level of DmMANF in two types of glia, astrocyte-like glia (AlGl) and ensheathing glia (EnGl), affect activity and sleep of flies. Interestingly, a proper level of DmMANF in AlGl seems to be important in guiding processes of pigment dispersing factor (PDF)-expressing clock neurons. This is supported by our finding that DmMANF overexpression in AlGl leads to structural changes in the architecture of the PDF-positive arborization in the brain. Finally, we detected that DmMANF also affects rhythms in glia themselves, as circadian oscillations in expression of the catalytic alpha subunit of the sodium pump in the lamina epithelial glia were abolished after DmMANF silencing. DmMANF expressed in AlGl and EnGl seems to affect the activity of neurons leading to changes in behaviour.pl
dc.affiliationWydział Biologii : Instytut Zoologii i Badań Biomedycznychpl
dc.contributor.authorWalkowicz, Lucyna - 164547 pl
dc.contributor.authorKrzeptowski, Wojciech - 104475 pl
dc.contributor.authorKrzeptowska, Ewelinapl
dc.contributor.authorWarzecha, Karolinapl
dc.contributor.authorSałek, Joannapl
dc.contributor.authorGórska-Andrzejak, Jolanta - 128153 pl
dc.contributor.authorPyza, Elżbieta - 131603 pl
dc.date.accessioned2021-11-15T13:28:43Z
dc.date.available2021-11-15T13:28:43Z
dc.date.issued2021pl
dc.description.additionalBibliogr. s. 5796-5797pl
dc.description.number5pl
dc.description.physical5785-5797pl
dc.description.volume54pl
dc.identifier.doi10.1111/ejn.15171pl
dc.identifier.eissn1460-9568pl
dc.identifier.issn0953-816Xpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/283663
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subject.enastrocyte-like gliapl
dc.subject.encircadian clockpl
dc.subject.enensheathing gliapl
dc.subject.englial oscillatorspl
dc.subject.envisual systempl
dc.subtypeArticlepl
dc.titleGlial expression of DmMANF is required for the regulation of activity, sleep and circadian rhythms in the visual system of Drosophila melanogasterpl
dc.title.journalEuropean Journal of Neurosciencepl
dc.title.volumeSpecial issue : glial plasticity in health and diseasepl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T00:14:34Z
dc.abstract.enpl
DmMANF, Drosophila melanogaster mesencephalic astrocyte-derived neurotrophic factor (DmMANF) is an evolutionarily conserved orthologue of mammalian MANF. This neurotrophic factor exerts many functions in the Drosophila brain, particularly those dependent on glial cells. As we found in our earlier study, downregulation of DmMANF in glia induces degeneration of glial cells in the first optic neuropil (lamina) where DmMANF abundance is especially high. In the present study, we observed that changes in the level of DmMANF in two types of glia, astrocyte-like glia (AlGl) and ensheathing glia (EnGl), affect activity and sleep of flies. Interestingly, a proper level of DmMANF in AlGl seems to be important in guiding processes of pigment dispersing factor (PDF)-expressing clock neurons. This is supported by our finding that DmMANF overexpression in AlGl leads to structural changes in the architecture of the PDF-positive arborization in the brain. Finally, we detected that DmMANF also affects rhythms in glia themselves, as circadian oscillations in expression of the catalytic alpha subunit of the sodium pump in the lamina epithelial glia were abolished after DmMANF silencing. DmMANF expressed in AlGl and EnGl seems to affect the activity of neurons leading to changes in behaviour.
dc.affiliationpl
Wydział Biologii : Instytut Zoologii i Badań Biomedycznych
dc.contributor.authorpl
Walkowicz, Lucyna - 164547
dc.contributor.authorpl
Krzeptowski, Wojciech - 104475
dc.contributor.authorpl
Krzeptowska, Ewelina
dc.contributor.authorpl
Warzecha, Karolina
dc.contributor.authorpl
Sałek, Joanna
dc.contributor.authorpl
Górska-Andrzejak, Jolanta - 128153
dc.contributor.authorpl
Pyza, Elżbieta - 131603
dc.date.accessioned
2021-11-15T13:28:43Z
dc.date.available
2021-11-15T13:28:43Z
dc.date.issuedpl
2021
dc.description.additionalpl
Bibliogr. s. 5796-5797
dc.description.numberpl
5
dc.description.physicalpl
5785-5797
dc.description.volumepl
54
dc.identifier.doipl
10.1111/ejn.15171
dc.identifier.eissnpl
1460-9568
dc.identifier.issnpl
0953-816X
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/283663
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
bez licencji
dc.rights.uri*
dc.subject.enpl
astrocyte-like glia
dc.subject.enpl
circadian clock
dc.subject.enpl
ensheathing glia
dc.subject.enpl
glial oscillators
dc.subject.enpl
visual system
dc.subtypepl
Article
dc.titlepl
Glial expression of DmMANF is required for the regulation of activity, sleep and circadian rhythms in the visual system of Drosophila melanogaster
dc.title.journalpl
European Journal of Neuroscience
dc.title.volumepl
Special issue : glial plasticity in health and disease
dc.typepl
JournalArticle
dspace.entity.type
Publication

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