Increased nitric oxide availability attenuates high fat diet metabolic alterations and gene expression associated with insulin resistance

2011
journal article
article
dc.abstract.enBackground High fat diet impairs nitric oxide (NO) bioavailability, and induces insulin resistance. The link between NO availability and the metabolic adaptation to a high fat diet is not well characterized. The purpose of this study was to investigate the effect of high fat diet on metabolism in mice with decreased (eNOS-/-) and increased (DDAH overexpressed) NO bioavailability. Methods eNOS-/- (n = 16), DDAH (n = 24), and WT (n = 19) mice were fed a high fat diet (HFD) for 13 weeks. Body weight, biochemical parameters, adipokines and insulin were monitored. The matrigel in vivo model with CD31 immunostaining was used to assess angiogenesis. Gene expression in adipose tissues was analyzed by microarray and Real Time PCR. Comparisons of the mean values were made using the unpaired Student t test and p < 0.05 were considered statistically significant. Results eNOS-/- mice gained less weight than control WT and DDAH mice. In DDAH mice, a greater increase in serum adiponectin and a lesser increment in glucose level was observed. Fasting insulin and cholesterol levels remained unchanged. The angiogenic response was increased in DDAH mice. In adipose tissue of DDAH mice, genes characteristic of differentiated adipocytes were down-regulated, whereas in eNOS-/- mice, genes associated with adipogenesis, fatty acid and triglyceride synthesis were upregulated. Conclusions Our results indicate that increased NO availability attenuates some HFD induced alterations in metabolism and gene expression associated with insulin resistance.pl
dc.affiliationWydział Lekarski : Klinika Chorób Metabolicznychpl
dc.affiliationWydział Lekarski : Zakład Diagnostykipl
dc.affiliationWydział Lekarski : Zakład Patomorfologii Klinicznej i Doświadczalnejpl
dc.affiliationWydział Lekarski : Zakład Diagnostyki Genetycznej i Nutrigenomikipl
dc.affiliationWydział Lekarski : Zakład Biochemii Klinicznejpl
dc.contributor.authorRaźny, Urszula - 133265 pl
dc.contributor.authorKieć-Wilk, Beata - 130090 pl
dc.contributor.authorWątor, Łukasz - 148304 pl
dc.contributor.authorPolus, Anna - 133199 pl
dc.contributor.authorDyduch, Grzegorz - 129300 pl
dc.contributor.authorSolnica, Bogdan - 133453 pl
dc.contributor.authorMałecki, Maciej - 130840 pl
dc.contributor.authorTomaszewska, Romana - 133668 pl
dc.contributor.authorCooke, John Ppl
dc.contributor.authorDembińska-Kieć, Aldona - 129163 pl
dc.date.accession2020-04-03pl
dc.date.accessioned2020-04-03T07:18:25Z
dc.date.available2020-04-03T07:18:25Z
dc.date.issued2011pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.publication1,34pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume10pl
dc.identifier.articleid68pl
dc.identifier.eissn1475-2840pl
dc.identifier.issn1475-2840pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/153134
dc.identifier.weblinkhttp://www.cardiab.com/content/10/1/68pl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 2.0*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/pl/legalcode*
dc.share.typeotwarte czasopismo
dc.subject.eneNOS-/- micepl
dc.subject.enDDAH micepl
dc.subject.enmicroarraypl
dc.subject.enadipogenesispl
dc.subject.enangiogenesispl
dc.subtypeArticlepl
dc.titleIncreased nitric oxide availability attenuates high fat diet metabolic alterations and gene expression associated with insulin resistancepl
dc.title.journalCardiovascular Diabetologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Background High fat diet impairs nitric oxide (NO) bioavailability, and induces insulin resistance. The link between NO availability and the metabolic adaptation to a high fat diet is not well characterized. The purpose of this study was to investigate the effect of high fat diet on metabolism in mice with decreased (eNOS-/-) and increased (DDAH overexpressed) NO bioavailability. Methods eNOS-/- (n = 16), DDAH (n = 24), and WT (n = 19) mice were fed a high fat diet (HFD) for 13 weeks. Body weight, biochemical parameters, adipokines and insulin were monitored. The matrigel in vivo model with CD31 immunostaining was used to assess angiogenesis. Gene expression in adipose tissues was analyzed by microarray and Real Time PCR. Comparisons of the mean values were made using the unpaired Student t test and p < 0.05 were considered statistically significant. Results eNOS-/- mice gained less weight than control WT and DDAH mice. In DDAH mice, a greater increase in serum adiponectin and a lesser increment in glucose level was observed. Fasting insulin and cholesterol levels remained unchanged. The angiogenic response was increased in DDAH mice. In adipose tissue of DDAH mice, genes characteristic of differentiated adipocytes were down-regulated, whereas in eNOS-/- mice, genes associated with adipogenesis, fatty acid and triglyceride synthesis were upregulated. Conclusions Our results indicate that increased NO availability attenuates some HFD induced alterations in metabolism and gene expression associated with insulin resistance.
dc.affiliationpl
Wydział Lekarski : Klinika Chorób Metabolicznych
dc.affiliationpl
Wydział Lekarski : Zakład Diagnostyki
dc.affiliationpl
Wydział Lekarski : Zakład Patomorfologii Klinicznej i Doświadczalnej
dc.affiliationpl
Wydział Lekarski : Zakład Diagnostyki Genetycznej i Nutrigenomiki
dc.affiliationpl
Wydział Lekarski : Zakład Biochemii Klinicznej
dc.contributor.authorpl
Raźny, Urszula - 133265
dc.contributor.authorpl
Kieć-Wilk, Beata - 130090
dc.contributor.authorpl
Wątor, Łukasz - 148304
dc.contributor.authorpl
Polus, Anna - 133199
dc.contributor.authorpl
Dyduch, Grzegorz - 129300
dc.contributor.authorpl
Solnica, Bogdan - 133453
dc.contributor.authorpl
Małecki, Maciej - 130840
dc.contributor.authorpl
Tomaszewska, Romana - 133668
dc.contributor.authorpl
Cooke, John P
dc.contributor.authorpl
Dembińska-Kieć, Aldona - 129163
dc.date.accessionpl
2020-04-03
dc.date.accessioned
2020-04-03T07:18:25Z
dc.date.available
2020-04-03T07:18:25Z
dc.date.issuedpl
2011
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.publicationpl
1,34
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
10
dc.identifier.articleidpl
68
dc.identifier.eissnpl
1475-2840
dc.identifier.issnpl
1475-2840
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/153134
dc.identifier.weblinkpl
http://www.cardiab.com/content/10/1/68
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 2.0
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/2.0/pl/legalcode
dc.share.type
otwarte czasopismo
dc.subject.enpl
eNOS-/- mice
dc.subject.enpl
DDAH mice
dc.subject.enpl
microarray
dc.subject.enpl
adipogenesis
dc.subject.enpl
angiogenesis
dc.subtypepl
Article
dc.titlepl
Increased nitric oxide availability attenuates high fat diet metabolic alterations and gene expression associated with insulin resistance
dc.title.journalpl
Cardiovascular Diabetology
dc.typepl
JournalArticle
dspace.entity.type
Publication

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