Perioperative changes in lymphocyte subpopulations in patients undergoing surgery for colorectal cancer

2019
journal article
article
5
cris.lastimport.wos2024-04-09T22:26:12Z
dc.abstract.enSurgical procedure has immense impact on the immune balance. However, little is known about perioperative changes in T regulatory and Th17 lymphocyte subpopulations in patients undergoing colorectal resection. Patients with resectable colon cancer were enrolled in the study. Blood samples were obtained on two occasions, i.e. before the procedure and two days after the surgery. We also recruited a control group of young, healthy individuals. Lymphocyte subpopulations were analyzed with the use of flow cytometry. Investigated subpopulations consisted of total lymphocyte count, CD4+, CD8+, T regulatory Foxp3+ (Tregs), Th17 lymphocytes and white blood cell (WBC) count. There were significant differences in immune cell levels before and after the surgery. Reduction was recorded in the CD4+, CD8+, Tregs and total lymphocyte counts (p=0.002, p=0.01, p=0.008 and p=0.001, respectively). Increase was observed in total WBC and Th17 cells, however, Th17 lymphocytes did not reach statistical significance (p=0.01 and p=0.5, respectively). In conclusion, surgical intervention caused changes in all lymphocyte subpopulations investigated in patients undergoing surgery for colorectal cancer. However, it seemed to be an effect of perioperative trauma. Further studies are needed to investigate the impact of surgical intervention on lymphocyte subpopulations.pl
dc.abstract.otherKirurški zahvat ima golem učinak na ravnotežu imunog sustava. Međutim, malo se zna o perioperacijskim promjenama u subpopulacijama T regulatornih i Th17 limfocita u bolesnika podvrgnutih resekciji kolorektuma. U istraživanje su bili uključeni bolesnici s resektabilnim karcinomom kolona. Uzorci krvi prikupljeni su dva puta: prije zahvata i dva dana nakon operacije. Uključena je bila i kontrolna skupina sastavljena od mladih i zdravih osoba. Subpopulacije limfocita analizirane su protočnom citometrijom. Istraživane su sljedeće subpopulacije: ukupan broj limfocita, CD4+, CD8+, T regulatorni Foxp3+ (Tregs), Th17 i bijela krvna slika. Utvrđene su značajne razlike u razinama imuno stanica prije i nakon operacije. Snižene razine zabilježene su za CD4+, CD8+, Tregs i ukupni broj limfocita (p=0,002, p=0,01, p=0,008 odnosno p=0,001). Povišenje je zapaženo za bijelu krvnu sliku i Th17 stanice, međutim, Th17 limfociti nisu postigli statističku značajnost (p=0,01 odnosno p=0,5). Zaključujemo da je kirurški zahvat uzrokovao promjene u svim istraživanim subpopulacijama limfocita kod bolesnika podvrgnutih operaciji kolorektalnog karcinoma. Međutim, čini se da je to bio učinak perioperacijske traume. Potrebna su daljnja istraživanja kako bi se ispitao utjecaj kirurškog zahvata na subpopulacije limfocita.pl
dc.affiliationWydział Lekarski : Instytut Pediatriipl
dc.affiliationWydział Lekarski : Klinika Chirurgii Ogólnej, Onkologicznej i Metabolicznejpl
dc.cm.date2020-12-02
dc.cm.id95599
dc.contributor.authorRubinkiewicz, Mateusz - 114793 pl
dc.contributor.authorSiemińska, Izabelapl
dc.contributor.authorMałczak, Piotr - 176001 pl
dc.contributor.authorMajor, Piotr - 141443 pl
dc.contributor.authorBaran, Jarosław - 128667 pl
dc.contributor.authorBudzyński, Andrzej - 128910 pl
dc.contributor.authorPędziwiatr, Michał - 140831 pl
dc.date.accession2020-06-18pl
dc.date.accessioned2020-12-02T10:07:34Zpl
dc.date.available2020-12-02T10:07:34Zpl
dc.date.issued2019pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number2pl
dc.description.physical337-342pl
dc.description.points20pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume58pl
dc.identifier.doi10.20471/acc.2019.58.02.18pl
dc.identifier.eissn1333-9451pl
dc.identifier.issn0353-9466pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/255983
dc.identifier.weblinkhttps://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=327511pl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa*
dc.rights.licenceCC-BY-NC-ND
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enLymphocyte subsetspl
dc.subject.enT-lymphocytespl
dc.subject.enregulatorypl
dc.subject.enTh17 cellspl
dc.subject.enLymphocyte countpl
dc.subject.enColorectal surgerypl
dc.subject.otherSubpopulacije limfocitapl
dc.subject.otherT-limfocitipl
dc.subject.otherregulacijskipl
dc.subject.otherTh17 stanicepl
dc.subject.otherBroj limfocitapl
dc.subject.otherKolorektalna kirurgijapl
dc.subtypeArticlepl
dc.titlePerioperative changes in lymphocyte subpopulations in patients undergoing surgery for colorectal cancerpl
dc.title.journalActa Clinica Croaticapl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T22:26:12Z
dc.abstract.enpl
Surgical procedure has immense impact on the immune balance. However, little is known about perioperative changes in T regulatory and Th17 lymphocyte subpopulations in patients undergoing colorectal resection. Patients with resectable colon cancer were enrolled in the study. Blood samples were obtained on two occasions, i.e. before the procedure and two days after the surgery. We also recruited a control group of young, healthy individuals. Lymphocyte subpopulations were analyzed with the use of flow cytometry. Investigated subpopulations consisted of total lymphocyte count, CD4+, CD8+, T regulatory Foxp3+ (Tregs), Th17 lymphocytes and white blood cell (WBC) count. There were significant differences in immune cell levels before and after the surgery. Reduction was recorded in the CD4+, CD8+, Tregs and total lymphocyte counts (p=0.002, p=0.01, p=0.008 and p=0.001, respectively). Increase was observed in total WBC and Th17 cells, however, Th17 lymphocytes did not reach statistical significance (p=0.01 and p=0.5, respectively). In conclusion, surgical intervention caused changes in all lymphocyte subpopulations investigated in patients undergoing surgery for colorectal cancer. However, it seemed to be an effect of perioperative trauma. Further studies are needed to investigate the impact of surgical intervention on lymphocyte subpopulations.
dc.abstract.otherpl
Kirurški zahvat ima golem učinak na ravnotežu imunog sustava. Međutim, malo se zna o perioperacijskim promjenama u subpopulacijama T regulatornih i Th17 limfocita u bolesnika podvrgnutih resekciji kolorektuma. U istraživanje su bili uključeni bolesnici s resektabilnim karcinomom kolona. Uzorci krvi prikupljeni su dva puta: prije zahvata i dva dana nakon operacije. Uključena je bila i kontrolna skupina sastavljena od mladih i zdravih osoba. Subpopulacije limfocita analizirane su protočnom citometrijom. Istraživane su sljedeće subpopulacije: ukupan broj limfocita, CD4+, CD8+, T regulatorni Foxp3+ (Tregs), Th17 i bijela krvna slika. Utvrđene su značajne razlike u razinama imuno stanica prije i nakon operacije. Snižene razine zabilježene su za CD4+, CD8+, Tregs i ukupni broj limfocita (p=0,002, p=0,01, p=0,008 odnosno p=0,001). Povišenje je zapaženo za bijelu krvnu sliku i Th17 stanice, međutim, Th17 limfociti nisu postigli statističku značajnost (p=0,01 odnosno p=0,5). Zaključujemo da je kirurški zahvat uzrokovao promjene u svim istraživanim subpopulacijama limfocita kod bolesnika podvrgnutih operaciji kolorektalnog karcinoma. Međutim, čini se da je to bio učinak perioperacijske traume. Potrebna su daljnja istraživanja kako bi se ispitao utjecaj kirurškog zahvata na subpopulacije limfocita.
dc.affiliationpl
Wydział Lekarski : Instytut Pediatrii
dc.affiliationpl
Wydział Lekarski : Klinika Chirurgii Ogólnej, Onkologicznej i Metabolicznej
dc.cm.date
2020-12-02
dc.cm.id
95599
dc.contributor.authorpl
Rubinkiewicz, Mateusz - 114793
dc.contributor.authorpl
Siemińska, Izabela
dc.contributor.authorpl
Małczak, Piotr - 176001
dc.contributor.authorpl
Major, Piotr - 141443
dc.contributor.authorpl
Baran, Jarosław - 128667
dc.contributor.authorpl
Budzyński, Andrzej - 128910
dc.contributor.authorpl
Pędziwiatr, Michał - 140831
dc.date.accessionpl
2020-06-18
dc.date.accessionedpl
2020-12-02T10:07:34Z
dc.date.availablepl
2020-12-02T10:07:34Z
dc.date.issuedpl
2019
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
2
dc.description.physicalpl
337-342
dc.description.pointspl
20
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
58
dc.identifier.doipl
10.20471/acc.2019.58.02.18
dc.identifier.eissnpl
1333-9451
dc.identifier.issnpl
0353-9466
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/255983
dc.identifier.weblinkpl
https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=327511
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa
dc.rights.licence
CC-BY-NC-ND
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
Lymphocyte subsets
dc.subject.enpl
T-lymphocytes
dc.subject.enpl
regulatory
dc.subject.enpl
Th17 cells
dc.subject.enpl
Lymphocyte count
dc.subject.enpl
Colorectal surgery
dc.subject.otherpl
Subpopulacije limfocita
dc.subject.otherpl
T-limfociti
dc.subject.otherpl
regulacijski
dc.subject.otherpl
Th17 stanice
dc.subject.otherpl
Broj limfocita
dc.subject.otherpl
Kolorektalna kirurgija
dc.subtypepl
Article
dc.titlepl
Perioperative changes in lymphocyte subpopulations in patients undergoing surgery for colorectal cancer
dc.title.journalpl
Acta Clinica Croatica
dc.typepl
JournalArticle
dspace.entity.type
Publication

* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.

Views
16
Views per month
Views per city
Ashburn
2
Chandler
2
Dublin
2
Wroclaw
2
Downloads
rubinkiewicz_et-al_perioperative_changes_in_lymphocyte_2019.odt
1
rubinkiewicz_et-al_perioperative_changes_in_lymphocyte_2019.pdf
1