Experimental and analytical procedures for ToF-SIMS measurement data of membranous structures

2023
journal article
article
cris.lastimport.wos2024-04-09T22:26:10Z
dc.abstract.enTime-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful analytical technique with great application potential in biomolecular matter research. SIMS measurements performed on biological samples, due to their complex structure and the content of many atomic, small and large molecular compounds, suffer very rich and complex mass spectra of particles, which characterise the content and physio-chemical properties of examined samples. The proper description and understanding of features appearing in the spectra, and, consequently, the final data confirming or rejecting the hypothesis put forward in the experiment, largely depend on the experimenter's correct understanding of the technique itself and its limitations, knowledge of the tested material and its appropriate preparation. These issues mean that obtaining the right answer to the questions posed in the research hypothesis requires not only the correct conduct of experiments but also the appropriate processing of post-experimental data. This study aims to demonstrate the impact of various analytical and experimental procedures applied to reach proper conclusions from TOF-SIM measurements. These are different types of data normalization, the selection of a so-called Region Of Interest (ROI), the selection of representative secondary ions, and specific quantification methods including a combination of experimental parameters. All these aspects were checked and discussed based on the results of the analysis of pancreatic β cells placed in a PBS solution on silicon wafers.pl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.contributor.authorSkalska, Magdalena - 166761 pl
dc.contributor.authorDurak-Kozica, Martyna - 148761 pl
dc.date.accessioned2024-01-30T08:37:21Z
dc.date.available2024-01-30T08:37:21Z
dc.date.issued2023pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number1pl
dc.description.physical64-68pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume19pl
dc.identifier.doi10.5604/01.3001.0054.1935pl
dc.identifier.eissn1896-530Xpl
dc.identifier.issn1895-9091pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/326432
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enToF-SIMSpl
dc.subject.enmembranous structurespl
dc.subject.endata preparationpl
dc.subject.endata acquisitionpl
dc.subject.ensecondary ion mass spectrometry experimentspl
dc.subtypeArticlepl
dc.titleExperimental and analytical procedures for ToF-SIMS measurement data of membranous structurespl
dc.title.journalBio-Algorithms and Med-Systemspl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T22:26:10Z
dc.abstract.enpl
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful analytical technique with great application potential in biomolecular matter research. SIMS measurements performed on biological samples, due to their complex structure and the content of many atomic, small and large molecular compounds, suffer very rich and complex mass spectra of particles, which characterise the content and physio-chemical properties of examined samples. The proper description and understanding of features appearing in the spectra, and, consequently, the final data confirming or rejecting the hypothesis put forward in the experiment, largely depend on the experimenter's correct understanding of the technique itself and its limitations, knowledge of the tested material and its appropriate preparation. These issues mean that obtaining the right answer to the questions posed in the research hypothesis requires not only the correct conduct of experiments but also the appropriate processing of post-experimental data. This study aims to demonstrate the impact of various analytical and experimental procedures applied to reach proper conclusions from TOF-SIM measurements. These are different types of data normalization, the selection of a so-called Region Of Interest (ROI), the selection of representative secondary ions, and specific quantification methods including a combination of experimental parameters. All these aspects were checked and discussed based on the results of the analysis of pancreatic β cells placed in a PBS solution on silicon wafers.
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
dc.contributor.authorpl
Skalska, Magdalena - 166761
dc.contributor.authorpl
Durak-Kozica, Martyna - 148761
dc.date.accessioned
2024-01-30T08:37:21Z
dc.date.available
2024-01-30T08:37:21Z
dc.date.issuedpl
2023
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
1
dc.description.physicalpl
64-68
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
19
dc.identifier.doipl
10.5604/01.3001.0054.1935
dc.identifier.eissnpl
1896-530X
dc.identifier.issnpl
1895-9091
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/326432
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
ToF-SIMS
dc.subject.enpl
membranous structures
dc.subject.enpl
data preparation
dc.subject.enpl
data acquisition
dc.subject.enpl
secondary ion mass spectrometry experiments
dc.subtypepl
Article
dc.titlepl
Experimental and analytical procedures for ToF-SIMS measurement data of membranous structures
dc.title.journalpl
Bio-Algorithms and Med-Systems
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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