Experimental data for paper : the acid-base/deprotonation equilibrium can be studied with a microscale thermophoresis (MST)

2022
research data
collection
cris.lastimport.wos2024-04-10T00:35:10Z
dc.abstract.enMicroscale thermophoresis (MST) is a rapidly developing bioanalytical technique used routinely for the examination of ligand-target affinity. It has never been used so far for the analysis of acid-base dissociation and determination of pKa constant. This work is the-proof-of-concept of this new idea. It demonstrates that the pKa values obtained from the thermophoretic data are consistent with the reference methods. As a result, the analytical potential and utility of the MST technology can become even greater, especially if the new detection system of thermophoretic movement will be developed in the future. Even now, taking into account the necessity to use fluorescence, the proposed method may be useful in many respects.pl
dc.affiliationWydział Chemii : Zakład Chemii Analitycznejpl
dc.contributor.authorNowak, Paweł - 149463 pl
dc.contributor.authorWoźniakiewicz, Michał - 142043 pl
dc.contributor.institutionZakład Chemii Analitycznej, Wydział Chemii, Uniwersytet Jagiellońskipl
dc.coverage.temporal2020-07-14 – 2024-07-13pl
dc.date.accessioned2022-01-03T13:00:26Z
dc.date.available2022-01-03T13:00:26Z
dc.date.issued2022pl
dc.description.additionalData acquired during the microscale thermophoresis study on determination of pKa values of FITC. Data are accompanied with the results of laser induced fluorescent measurements on pH scale using the CE-LIF instrumentation. Finally data are accompanied with the result form the preliminary study: the recorded fluorescence signals for FITC with the MST instrument in response to the change in pH value: before turning on the IR laser (top), and at three different MST powers corresponding to different temperature gradients formed as a function of time (bottom).pl
dc.description.grantnumber2019/35/B/ST4/01022pl
dc.description.granttitleComprehensive evaluation of the analytical capabilities of a capillary electrophoresis coupled with microscale thermophoresis (CE-MST)pl
dc.description.sponsorshipNarodowe Centrum Nauki (NCN)pl
dc.description.version1pl
dc.identifier.doi10.26106/p7x8-y113pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/286417
dc.languageengpl
dc.pbn.affiliationDziedzina nauk ścisłych i przyrodniczych : nauki chemicznepl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.subject.enacid-base equilibriumpl
dc.subject.enfluorescein isothiocyanatepl
dc.subject.enfluorescencepl
dc.subject.enmicroScale thermophoresispl
dc.subject.enthermal diffusionpl
dc.subject.enthermodynamicspl
dc.subtypeCollectionpl
dc.titleExperimental data for paper : the acid-base/deprotonation equilibrium can be studied with a microscale thermophoresis (MST)pl
dc.typeResearchDatapl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T00:35:10Z
dc.abstract.enpl
Microscale thermophoresis (MST) is a rapidly developing bioanalytical technique used routinely for the examination of ligand-target affinity. It has never been used so far for the analysis of acid-base dissociation and determination of pKa constant. This work is the-proof-of-concept of this new idea. It demonstrates that the pKa values obtained from the thermophoretic data are consistent with the reference methods. As a result, the analytical potential and utility of the MST technology can become even greater, especially if the new detection system of thermophoretic movement will be developed in the future. Even now, taking into account the necessity to use fluorescence, the proposed method may be useful in many respects.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Analitycznej
dc.contributor.authorpl
Nowak, Paweł - 149463
dc.contributor.authorpl
Woźniakiewicz, Michał - 142043
dc.contributor.institutionpl
Zakład Chemii Analitycznej, Wydział Chemii, Uniwersytet Jagielloński
dc.coverage.temporalpl
2020-07-14 – 2024-07-13
dc.date.accessioned
2022-01-03T13:00:26Z
dc.date.available
2022-01-03T13:00:26Z
dc.date.issuedpl
2022
dc.description.additionalpl
Data acquired during the microscale thermophoresis study on determination of pKa values of FITC. Data are accompanied with the results of laser induced fluorescent measurements on pH scale using the CE-LIF instrumentation. Finally data are accompanied with the result form the preliminary study: the recorded fluorescence signals for FITC with the MST instrument in response to the change in pH value: before turning on the IR laser (top), and at three different MST powers corresponding to different temperature gradients formed as a function of time (bottom).
dc.description.grantnumberpl
2019/35/B/ST4/01022
dc.description.granttitlepl
Comprehensive evaluation of the analytical capabilities of a capillary electrophoresis coupled with microscale thermophoresis (CE-MST)
dc.description.sponsorshippl
Narodowe Centrum Nauki (NCN)
dc.description.versionpl
1
dc.identifier.doipl
10.26106/p7x8-y113
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/286417
dc.languagepl
eng
dc.pbn.affiliationpl
Dziedzina nauk ścisłych i przyrodniczych : nauki chemiczne
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.subject.enpl
acid-base equilibrium
dc.subject.enpl
fluorescein isothiocyanate
dc.subject.enpl
fluorescence
dc.subject.enpl
microScale thermophoresis
dc.subject.enpl
thermal diffusion
dc.subject.enpl
thermodynamics
dc.subtypepl
Collection
dc.titlepl
Experimental data for paper : the acid-base/deprotonation equilibrium can be studied with a microscale thermophoresis (MST)
dc.typepl
ResearchData
dspace.entity.type
Publication
Affiliations

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