Stratification, scarification and application of phytohormones promote dormancy breaking and germination of pelleted Scots pine (Pinus sylvestris L.) seeds

2021
journal article
article
9
cris.lastimport.wos2024-04-09T22:27:25Z
dc.abstract.enSeed quality is an important issue in forestry as it is an essential parameter in the production of high quality planting material. Many factors may hinder the harvesting of high quality seeds, including an insufficient number of sunny days, external conditions in temperate climate zones, and fungal pathogens affecting development of seedlings. We undertook to develop a procedure maximizing seed protection and promoting the optimum physiological development of seedlings by examination of the impact of seed pelleting (a general seed protection method) on germination rates and seedling development of Scots pine (Pinus sylvestris L.). Germination of pelleted seeds was examined in relation to substrate (water vs. soil) and LED light spectrum (white vs. red-blue). Several dormancy breaking treatments were applied: stratification/scarification, and growth regulator treatments including gibberellic acid ($GA_{3}$), indole-3-acetic acid (IAA) and 1-naphthaleneacetic acid (NAA), to stimulate seed germination. Experiments included independent tests for each treatment (stratification/scarification and plant growth regulators), and combinations of both stratification/scarification and phytohormone treatments. The impacts of these treatments and various controlled germination conditions on the fluorescence of chlorophyll were analyzed using the maximum efficiency of photosystem II photochemistry parameter (Fv/Fm). In addition, chlorophyll a and b content in Scots pine seedlings germinated from pelleted seeds, were quantified using high-performance liquid chromatography (HPLC). The combined stratification/scarification and growth regulator treatment was the most effective germination promoting method for pelleted Scots pine seeds. Scots pine seeds are highly likely to be photoblastic. The best germination rate, while maintaining optimal physiological parameters, was achieved in acidic soil (pH 5.0) with white LED light.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biochemii Roślinpl
dc.contributor.authorNawrot-Chorabik, Katarzynapl
dc.contributor.authorOsmenda, Małgorzatapl
dc.contributor.authorSlowiński, Krzysztofpl
dc.contributor.authorLatowski, Dariusz - 186402 pl
dc.contributor.authorTabor, Sylwesterpl
dc.contributor.authorWoodward, Stephenpl
dc.date.accessioned2021-10-25T11:12:58Z
dc.date.available2021-10-25T11:12:58Z
dc.date.issued2021pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number5pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume12pl
dc.identifier.articleid621pl
dc.identifier.doi10.3390/f12050621pl
dc.identifier.eissn1999-4907pl
dc.identifier.projectROD UJ / Opl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/281528
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.engerminationpl
dc.subject.enLED lightpl
dc.subject.enphotoblastic seedspl
dc.subject.enphytochromespl
dc.subject.enseed pelletingpl
dc.subtypeArticlepl
dc.titleStratification, scarification and application of phytohormones promote dormancy breaking and germination of pelleted Scots pine (Pinus sylvestris L.) seedspl
dc.title.journalForestspl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T22:27:25Z
dc.abstract.enpl
Seed quality is an important issue in forestry as it is an essential parameter in the production of high quality planting material. Many factors may hinder the harvesting of high quality seeds, including an insufficient number of sunny days, external conditions in temperate climate zones, and fungal pathogens affecting development of seedlings. We undertook to develop a procedure maximizing seed protection and promoting the optimum physiological development of seedlings by examination of the impact of seed pelleting (a general seed protection method) on germination rates and seedling development of Scots pine (Pinus sylvestris L.). Germination of pelleted seeds was examined in relation to substrate (water vs. soil) and LED light spectrum (white vs. red-blue). Several dormancy breaking treatments were applied: stratification/scarification, and growth regulator treatments including gibberellic acid ($GA_{3}$), indole-3-acetic acid (IAA) and 1-naphthaleneacetic acid (NAA), to stimulate seed germination. Experiments included independent tests for each treatment (stratification/scarification and plant growth regulators), and combinations of both stratification/scarification and phytohormone treatments. The impacts of these treatments and various controlled germination conditions on the fluorescence of chlorophyll were analyzed using the maximum efficiency of photosystem II photochemistry parameter (Fv/Fm). In addition, chlorophyll a and b content in Scots pine seedlings germinated from pelleted seeds, were quantified using high-performance liquid chromatography (HPLC). The combined stratification/scarification and growth regulator treatment was the most effective germination promoting method for pelleted Scots pine seeds. Scots pine seeds are highly likely to be photoblastic. The best germination rate, while maintaining optimal physiological parameters, was achieved in acidic soil (pH 5.0) with white LED light.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biochemii Roślin
dc.contributor.authorpl
Nawrot-Chorabik, Katarzyna
dc.contributor.authorpl
Osmenda, Małgorzata
dc.contributor.authorpl
Slowiński, Krzysztof
dc.contributor.authorpl
Latowski, Dariusz - 186402
dc.contributor.authorpl
Tabor, Sylwester
dc.contributor.authorpl
Woodward, Stephen
dc.date.accessioned
2021-10-25T11:12:58Z
dc.date.available
2021-10-25T11:12:58Z
dc.date.issuedpl
2021
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
5
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
12
dc.identifier.articleidpl
621
dc.identifier.doipl
10.3390/f12050621
dc.identifier.eissnpl
1999-4907
dc.identifier.projectpl
ROD UJ / O
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/281528
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
germination
dc.subject.enpl
LED light
dc.subject.enpl
photoblastic seeds
dc.subject.enpl
phytochromes
dc.subject.enpl
seed pelleting
dc.subtypepl
Article
dc.titlepl
Stratification, scarification and application of phytohormones promote dormancy breaking and germination of pelleted Scots pine (Pinus sylvestris L.) seeds
dc.title.journalpl
Forests
dc.typepl
JournalArticle
dspace.entity.type
Publication

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