Cationic protein 8 plays multiple roles in Galleria mellonella immunity

2022
journal article
article
6
dc.abstract.enGalleria mellonella cationic protein 8 (GmCP8) is a hemolymph protein previously identified as an opsonin and an inhibitor of fungal proteases. In this work, we showed its bactericidal activity toward Pseudomonas entomophila, Pseudomonas aeruginosa, Bacillus thuringiensis, Staphylococcus aureus, and Escherichia coli and against yeast-like fungi Candida albicans. The activity against E. coli was correlated with bacterial membrane permeabilization. In turn, in the case of P. entomophila, B. thuringiensis, and C. albicans, the atomic force microscopy analysis of the microbial surface showed changes in the topography of cells and changes in their nanomechanical properties. GmCP8 also showed the inhibitory activity toward the serine protease trypsin and the metalloproteinase thermolysin. The expression of the gene encoding the GmCP8 protein did not increase either in the gut or in the fat body of G. mellonella after oral infection with P. entomophila. Similarly, the amount of GmCP8 in the hemolymph of G. mellonella did not change in immune-challenged insects. However, when GmCP8 was injected into the G. mellonella hemocel, a change in the survival curve was observed in the infected larvae. Our results shed new light on the function of GmCP8 protein in insect immunity, indicating its role in humoral defence mechanisms.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznejpl
dc.affiliationSzkoła Doktorska Nauk Ścisłych i Przyrodniczychpl
dc.contributor.authorKordaczuk, Jakubpl
dc.contributor.authorSułek, Michałpl
dc.contributor.authorMak, Paweł - 130230 pl
dc.contributor.authorZdybicka-Barabas, Agnieszkapl
dc.contributor.authorŚmiałek-Bartyzel, Justyna - 246115 pl
dc.contributor.authorWojda, Iwonapl
dc.date.accessioned2023-01-16T08:51:18Z
dc.date.available2023-01-16T08:51:18Z
dc.date.issued2022pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. Justyna Śmiałek-Bartyzel podpisana Justyna Śmiałekpl
dc.description.versionostateczna wersja wydawcy
dc.description.volume12pl
dc.identifier.articleid11737pl
dc.identifier.doi10.1038/s41598-022-15929-6pl
dc.identifier.eissn2045-2322pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/306086
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subtypeArticlepl
dc.titleCationic protein 8 plays multiple roles in Galleria mellonella immunitypl
dc.title.journalScientific Reportspl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Galleria mellonella cationic protein 8 (GmCP8) is a hemolymph protein previously identified as an opsonin and an inhibitor of fungal proteases. In this work, we showed its bactericidal activity toward Pseudomonas entomophila, Pseudomonas aeruginosa, Bacillus thuringiensis, Staphylococcus aureus, and Escherichia coli and against yeast-like fungi Candida albicans. The activity against E. coli was correlated with bacterial membrane permeabilization. In turn, in the case of P. entomophila, B. thuringiensis, and C. albicans, the atomic force microscopy analysis of the microbial surface showed changes in the topography of cells and changes in their nanomechanical properties. GmCP8 also showed the inhibitory activity toward the serine protease trypsin and the metalloproteinase thermolysin. The expression of the gene encoding the GmCP8 protein did not increase either in the gut or in the fat body of G. mellonella after oral infection with P. entomophila. Similarly, the amount of GmCP8 in the hemolymph of G. mellonella did not change in immune-challenged insects. However, when GmCP8 was injected into the G. mellonella hemocel, a change in the survival curve was observed in the infected larvae. Our results shed new light on the function of GmCP8 protein in insect immunity, indicating its role in humoral defence mechanisms.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznej
dc.affiliationpl
Szkoła Doktorska Nauk Ścisłych i Przyrodniczych
dc.contributor.authorpl
Kordaczuk, Jakub
dc.contributor.authorpl
Sułek, Michał
dc.contributor.authorpl
Mak, Paweł - 130230
dc.contributor.authorpl
Zdybicka-Barabas, Agnieszka
dc.contributor.authorpl
Śmiałek-Bartyzel, Justyna - 246115
dc.contributor.authorpl
Wojda, Iwona
dc.date.accessioned
2023-01-16T08:51:18Z
dc.date.available
2023-01-16T08:51:18Z
dc.date.issuedpl
2022
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. Justyna Śmiałek-Bartyzel podpisana Justyna Śmiałek
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
12
dc.identifier.articleidpl
11737
dc.identifier.doipl
10.1038/s41598-022-15929-6
dc.identifier.eissnpl
2045-2322
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/306086
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
Cationic protein 8 plays multiple roles in Galleria mellonella immunity
dc.title.journalpl
Scientific Reports
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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