The dual role of heme oxygenase in regulating apoptosis in the nervous system of Drosophila melanogaster

2023
journal article
article
4
dc.abstract.enIncreasing evidence from mammalian studies suggests the Janus-face character of heme oxygenase (HO) in oxidative stress-dependent neurodegeneration. The present study aimed to investigate both the neuroprotective and neurotoxic effects of heme oxygenase (HO) after overexpressing or silencing the gene that encodes this protein in the nervous system of Drosophila melanogaster. Our data showed that HO can be pro-apoptotic or anti-apoptotic under different conditions. In young (7-day-old) flies, the apoptotic gene head involution defective (hid) mRNA and the activity of the initiator caspase Dronc increase in the fly’s head after pan-neuronal ho overexpression and pan-neuronal ho silencing as well. In addition, various expression levels of ho produce cell-specific degeneration. Dopaminergic neurons (DA) are particularly vulnerable to changes in ho expression, whereas eye photoreceptors are sensitive only to ho silencing. During aging, the expression of the hid apoptotic gene decreases in fly heads, along with the unprogressive degeneration of DA neurons, in transgenic strains with an increased or decreased HO level. However, we still observed significantly higher activity of caspase Dronc regardless of ho mRNA levels in neurons from older flies. Our results indicate that neuronal HO regulates apoptosis and this process depends on ho expression level, cell types, and age of flies.pl
dc.affiliationWydział Biologii : Instytut Zoologii i Badań Biomedycznychpl
dc.contributor.authorAbaquita, Terence Al - 411381 pl
dc.contributor.authorDamulewicz, Milena - 104424 pl
dc.contributor.authorTylko, Grzegorz - 132463 pl
dc.contributor.authorPyza, Elżbieta - 131603 pl
dc.date.accessioned2023-02-09T13:58:27Z
dc.date.available2023-02-09T13:58:27Z
dc.date.issued2023pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalOnline First 2023-01-30pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume14pl
dc.identifier.articleid1060175pl
dc.identifier.doi10.3389/fphys.2023.1060175pl
dc.identifier.eissn1664-042Xpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/307544
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enneuroprotectionpl
dc.subject.enneurotoxicitypl
dc.subject.enagingpl
dc.subject.enautophagypl
dc.subject.enoxidative stresspl
dc.subtypeArticlepl
dc.titleThe dual role of heme oxygenase in regulating apoptosis in the nervous system of Drosophila melanogasterpl
dc.title.journalFrontiers in Physiologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Increasing evidence from mammalian studies suggests the Janus-face character of heme oxygenase (HO) in oxidative stress-dependent neurodegeneration. The present study aimed to investigate both the neuroprotective and neurotoxic effects of heme oxygenase (HO) after overexpressing or silencing the gene that encodes this protein in the nervous system of Drosophila melanogaster. Our data showed that HO can be pro-apoptotic or anti-apoptotic under different conditions. In young (7-day-old) flies, the apoptotic gene head involution defective (hid) mRNA and the activity of the initiator caspase Dronc increase in the fly’s head after pan-neuronal ho overexpression and pan-neuronal ho silencing as well. In addition, various expression levels of ho produce cell-specific degeneration. Dopaminergic neurons (DA) are particularly vulnerable to changes in ho expression, whereas eye photoreceptors are sensitive only to ho silencing. During aging, the expression of the hid apoptotic gene decreases in fly heads, along with the unprogressive degeneration of DA neurons, in transgenic strains with an increased or decreased HO level. However, we still observed significantly higher activity of caspase Dronc regardless of ho mRNA levels in neurons from older flies. Our results indicate that neuronal HO regulates apoptosis and this process depends on ho expression level, cell types, and age of flies.
dc.affiliationpl
Wydział Biologii : Instytut Zoologii i Badań Biomedycznych
dc.contributor.authorpl
Abaquita, Terence Al - 411381
dc.contributor.authorpl
Damulewicz, Milena - 104424
dc.contributor.authorpl
Tylko, Grzegorz - 132463
dc.contributor.authorpl
Pyza, Elżbieta - 131603
dc.date.accessioned
2023-02-09T13:58:27Z
dc.date.available
2023-02-09T13:58:27Z
dc.date.issuedpl
2023
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Online First 2023-01-30
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
14
dc.identifier.articleidpl
1060175
dc.identifier.doipl
10.3389/fphys.2023.1060175
dc.identifier.eissnpl
1664-042X
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/307544
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
neuroprotection
dc.subject.enpl
neurotoxicity
dc.subject.enpl
aging
dc.subject.enpl
autophagy
dc.subject.enpl
oxidative stress
dc.subtypepl
Article
dc.titlepl
The dual role of heme oxygenase in regulating apoptosis in the nervous system of Drosophila melanogaster
dc.title.journalpl
Frontiers in Physiology
dc.typepl
JournalArticle
dspace.entity.type
Publication

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