Levels of plasma matrix metalloproteinases (MMP-2 and MMP-9) in response to INTEGRA® dermal regeneration template implantation

2014
journal article
article
cris.lastimport.wos2024-04-10T00:27:35Z
dc.abstract.enBackground : Cutaneous wound healing results in scar formation. Matrix metalloproteinases (MMP) transform extracellular matrix proteins and modulate inflammation and cell signaling, thus determining scar outcome. To provide rapid wound closure and reduced scarring, dermal scaffolds were introduced. Little is known about the influence of these materials on MMPs levels. Material and Methods : In this in vivo study the levels of MMP-2, MMP-9, and mediators of inflammation and fibrosis (IL-4 and TGF-beta1) in patients treated with Integra® dermal regeneration template (IDRT) were investigated. In the group of 11 pediatric patients treated with IDRT, levels of selected molecules were analyzed before surgery and at day 1, 7, and 25 after scaffold implantation. Results : The mean IDRT take rate was 89.5±4.7% with 4 patients (36%) who developed local infection. Patients were divided into 2 groups according to presence of infection (1 group with complications and 1 group without complications). In the group with complications, the IDRT take rate was significantly reduced compared to the group without complications (71.5±5.4 vs. 100±0.1; p<0.005). Plasma levels of MMP-2 were significantly (p<0.05) elevated in both groups on day 7 after the scaffold placement compared to baseline. Positive correlations between IL-4 and MMP-2 (p=0.01) in the group with complications and TGF-beta1 and MMP-9 (p=0.012) in both groups were observed. Conclusions : These findings suggest that Integra® scaffold degradation is mainly caused by MMP-2, whereas inflammation associated with local infection increases levels of this molecule and it is not associated with elevation of MMP-9. This shows that dermal regeneration with Integra® uses molecular mechanisms other than scar formation during dermal wound healing.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biologii Komórkipl
dc.affiliationWydział Nauk o Zdrowiu : Zakład Ratownictwa Medycznegopl
dc.cm.id86474
dc.contributor.authorNessler, Michał Bohdan - 131111 pl
dc.contributor.authorPuchała, Jacek - 133240 pl
dc.contributor.authorChrapusta, Annapl
dc.contributor.authorNessler, Katarzyna - 103970 pl
dc.contributor.authorDrukała, Justyna - 127766 pl
dc.date.accessioned2015-05-13T08:24:44Z
dc.date.available2015-05-13T08:24:44Z
dc.date.issued2014pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.physical91-96pl
dc.description.points10pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume20pl
dc.identifier.doi10.12659/MSM.889135pl
dc.identifier.eissn1643-3750pl
dc.identifier.issn1234-1010pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/6937
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa*
dc.rights.licenceCC-BY-NC-ND
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enwound healingpl
dc.subject.endermal scaffoldspl
dc.subject.enmetalloproteinasespl
dc.subject.endermal regenerationpl
dc.subject.enhumanspl
dc.subject.enfemalepl
dc.subject.enacellular dermis - metabolismpl
dc.subject.enmalepl
dc.subject.enmatrix metalloproteinase 9pl
dc.subject.enmatrix metalloproteinase 2pl
dc.subject.entissue scaffoldspl
dc.subtypeArticlepl
dc.titleLevels of plasma matrix metalloproteinases (MMP-2 and MMP-9) in response to INTEGRA® dermal regeneration template implantationpl
dc.title.journalMedical Science Monitorpl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T00:27:35Z
dc.abstract.enpl
Background : Cutaneous wound healing results in scar formation. Matrix metalloproteinases (MMP) transform extracellular matrix proteins and modulate inflammation and cell signaling, thus determining scar outcome. To provide rapid wound closure and reduced scarring, dermal scaffolds were introduced. Little is known about the influence of these materials on MMPs levels. Material and Methods : In this in vivo study the levels of MMP-2, MMP-9, and mediators of inflammation and fibrosis (IL-4 and TGF-beta1) in patients treated with Integra® dermal regeneration template (IDRT) were investigated. In the group of 11 pediatric patients treated with IDRT, levels of selected molecules were analyzed before surgery and at day 1, 7, and 25 after scaffold implantation. Results : The mean IDRT take rate was 89.5±4.7% with 4 patients (36%) who developed local infection. Patients were divided into 2 groups according to presence of infection (1 group with complications and 1 group without complications). In the group with complications, the IDRT take rate was significantly reduced compared to the group without complications (71.5±5.4 vs. 100±0.1; p<0.005). Plasma levels of MMP-2 were significantly (p<0.05) elevated in both groups on day 7 after the scaffold placement compared to baseline. Positive correlations between IL-4 and MMP-2 (p=0.01) in the group with complications and TGF-beta1 and MMP-9 (p=0.012) in both groups were observed. Conclusions : These findings suggest that Integra® scaffold degradation is mainly caused by MMP-2, whereas inflammation associated with local infection increases levels of this molecule and it is not associated with elevation of MMP-9. This shows that dermal regeneration with Integra® uses molecular mechanisms other than scar formation during dermal wound healing.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biologii Komórki
dc.affiliationpl
Wydział Nauk o Zdrowiu : Zakład Ratownictwa Medycznego
dc.cm.id
86474
dc.contributor.authorpl
Nessler, Michał Bohdan - 131111
dc.contributor.authorpl
Puchała, Jacek - 133240
dc.contributor.authorpl
Chrapusta, Anna
dc.contributor.authorpl
Nessler, Katarzyna - 103970
dc.contributor.authorpl
Drukała, Justyna - 127766
dc.date.accessioned
2015-05-13T08:24:44Z
dc.date.available
2015-05-13T08:24:44Z
dc.date.issuedpl
2014
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.physicalpl
91-96
dc.description.pointspl
10
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
20
dc.identifier.doipl
10.12659/MSM.889135
dc.identifier.eissnpl
1643-3750
dc.identifier.issnpl
1234-1010
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/6937
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa
dc.rights.licence
CC-BY-NC-ND
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
wound healing
dc.subject.enpl
dermal scaffolds
dc.subject.enpl
metalloproteinases
dc.subject.enpl
dermal regeneration
dc.subject.enpl
humans
dc.subject.enpl
female
dc.subject.enpl
acellular dermis - metabolism
dc.subject.enpl
male
dc.subject.enpl
matrix metalloproteinase 9
dc.subject.enpl
matrix metalloproteinase 2
dc.subject.enpl
tissue scaffolds
dc.subtypepl
Article
dc.titlepl
Levels of plasma matrix metalloproteinases (MMP-2 and MMP-9) in response to INTEGRA® dermal regeneration template implantation
dc.title.journalpl
Medical Science Monitor
dc.typepl
JournalArticle
dspace.entity.type
Publication

* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.

Views
12
Views per month
Views per city
Chandler
3
Ashburn
2
Wroclaw
2
Dublin
1
Downloads
nessler_puchala_chrapusta_nessler_drukala_levels_of_plasma_matrix_metalloproteinases_2014.odt
1