Furazidin (FUR) is a 5-nitrofuran derivative with a well-known and unique antibacterial activity, commonly applied in the treatment of urinary tract infections. FUR has been used regionally and globally in medicine, resulting in its continuous release into the natural ecosystem. Therefore, an accurate determination of trace levels of FUR in complex samples is highly reasonable and desirable. In this context, we propose a simple, fast, and highly sensitive electroanalytical method of FUR determination by means of differential pulse voltammetry with the use of Co-ferrierite/mesoporous carbon modified glassy carbon electrode (CoFER/MC-GCE). The advantageous features and electrochemical properties of CoFER/MC-GCE were confirmed by morphological and textural research, including X-ray fluorescence, Fourier-transform infrared spectroscopy, nitrogen adsorption/desorption method, and scanning electron microscopy coupled with energy dispersion spectroscopy measurements, as well as electrochemical studies involving cyclic voltammetry and electrochemical impedance spectroscopy (EIS). The linear response of CoFER/MC-GCE was achieved in the FUR concentration ranges of 0.02 − 0.29 mg and 0.33 − 0.48 mg with a detection limit of 3.9 μg . The acceptable results of pharmaceuticals, wastewater, and biological fluids analysis confirmed the analytical usefulness of the proposed voltammetric strategy of FUR determination using CoFER/MC-GCE.