Bystander activation of Bordetella pertussis-induced nasal tissue-resident memory CD4 T cells confers heterologous immunity to Klebsiella pneumoniae

2023
journal article
article
6
cris.lastimport.wos2024-04-09T19:39:11Z
dc.abstract.enAbstract Tissue-resident memory CD4 T ($T_{RM}$) cells induced by infection with Bordetella pertussis persist in respiratory tissues and confer long-term protective immunity against re-infection. However, it is not clear how they are maintained in respiratory tissues. Here we demonstrate that B. pertussis-specific CD4 $T_{RM}$ cells produce IL-17A in response to in vitro stimulation with LPS or heat-killed Klebsiella pneumoniae (HKKP) in the presence of dendritic cells. Furthermore, IL-17A-secreting CD4 $T_{RM}$ cells expand in the lung and nasal tissue of B. pertussis convalescent mice following in vivo administration of LPS or HKKP. Bystander activation of CD4 $T_{RM}$ cells was suppressed by anti-IL-12p40, but not by anti-MHCII antibodies. Furthermore, purified respiratory tissue-resident, but not circulating, CD4 T cells from convalescent mice produced IL-17A following direct stimulation with IL-23 and IL-1$\beta$ or IL-18. Intranasal immunization of mice with a whole cell pertussis vaccine induced respiratory CD4 $T_{RM}$ cells that were re-activated following stimulation with K. pneumoniae. Furthermore, the nasal pertussis vaccine conferred protective immunity against B. pertussis but also attenuated infection with K. pneumoniae. Our findings demonstrate CD4 $T_{RM}$ cells induced by respiratory infection or vaccination can undergo bystander activation and confer heterologous immunity to an unrelated respiratory pathogen.pl
dc.contributor.authorCurham, Lucy M.pl
dc.contributor.authorMannion, Jenny M.pl
dc.contributor.authorDaly, Clíodhna M.pl
dc.contributor.authorWilk, Mieszko - 445913 pl
dc.contributor.authorBorkner, Lisapl
dc.contributor.authorLalor, Stephen J.pl
dc.contributor.authorMcLoughlin, Rachel M.pl
dc.contributor.authorMills, Kingston H. G.pl
dc.date.accessioned2023-06-19T13:48:44Z
dc.date.available2023-06-19T13:48:44Z
dc.date.issued2023pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalMieszko Wilk podpisany: Mieszko M. Wilkpl
dc.description.number5pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume53pl
dc.identifier.articleid2250247pl
dc.identifier.doi10.1002/eji.202250247pl
dc.identifier.eissn1521-4141pl
dc.identifier.issn0014-2980pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/312423
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enbacterial immunitypl
dc.subject.enBordetella pertussispl
dc.subject.enheterologous immunitypl
dc.subject.entissue-resident memory T cellspl
dc.subject.envaccinespl
dc.subtypeArticlepl
dc.titleBystander activation of Bordetella pertussis-induced nasal tissue-resident memory CD4 T cells confers heterologous immunity to Klebsiella pneumoniaepl
dc.title.journalEuropean Journal of Immunologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T19:39:11Z
dc.abstract.enpl
Abstract Tissue-resident memory CD4 T ($T_{RM}$) cells induced by infection with Bordetella pertussis persist in respiratory tissues and confer long-term protective immunity against re-infection. However, it is not clear how they are maintained in respiratory tissues. Here we demonstrate that B. pertussis-specific CD4 $T_{RM}$ cells produce IL-17A in response to in vitro stimulation with LPS or heat-killed Klebsiella pneumoniae (HKKP) in the presence of dendritic cells. Furthermore, IL-17A-secreting CD4 $T_{RM}$ cells expand in the lung and nasal tissue of B. pertussis convalescent mice following in vivo administration of LPS or HKKP. Bystander activation of CD4 $T_{RM}$ cells was suppressed by anti-IL-12p40, but not by anti-MHCII antibodies. Furthermore, purified respiratory tissue-resident, but not circulating, CD4 T cells from convalescent mice produced IL-17A following direct stimulation with IL-23 and IL-1$\beta$ or IL-18. Intranasal immunization of mice with a whole cell pertussis vaccine induced respiratory CD4 $T_{RM}$ cells that were re-activated following stimulation with K. pneumoniae. Furthermore, the nasal pertussis vaccine conferred protective immunity against B. pertussis but also attenuated infection with K. pneumoniae. Our findings demonstrate CD4 $T_{RM}$ cells induced by respiratory infection or vaccination can undergo bystander activation and confer heterologous immunity to an unrelated respiratory pathogen.
dc.contributor.authorpl
Curham, Lucy M.
dc.contributor.authorpl
Mannion, Jenny M.
dc.contributor.authorpl
Daly, Clíodhna M.
dc.contributor.authorpl
Wilk, Mieszko - 445913
dc.contributor.authorpl
Borkner, Lisa
dc.contributor.authorpl
Lalor, Stephen J.
dc.contributor.authorpl
McLoughlin, Rachel M.
dc.contributor.authorpl
Mills, Kingston H. G.
dc.date.accessioned
2023-06-19T13:48:44Z
dc.date.available
2023-06-19T13:48:44Z
dc.date.issuedpl
2023
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Mieszko Wilk podpisany: Mieszko M. Wilk
dc.description.numberpl
5
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
53
dc.identifier.articleidpl
2250247
dc.identifier.doipl
10.1002/eji.202250247
dc.identifier.eissnpl
1521-4141
dc.identifier.issnpl
0014-2980
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/312423
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
bacterial immunity
dc.subject.enpl
Bordetella pertussis
dc.subject.enpl
heterologous immunity
dc.subject.enpl
tissue-resident memory T cells
dc.subject.enpl
vaccines
dc.subtypepl
Article
dc.titlepl
Bystander activation of Bordetella pertussis-induced nasal tissue-resident memory CD4 T cells confers heterologous immunity to Klebsiella pneumoniae
dc.title.journalpl
European Journal of Immunology
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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