Decreasing the thresholds for electroporation by sensitizing cells with local cationic anesthetics and substances that decrease the surface negative electric charge

2014
journal article
article
8
cris.lastimport.wos2024-04-09T19:42:46Z
dc.abstract.enThe recently described method of cell electroporation by flow of cell suspension through localized direct current electric fields (dcEFs) was applied to identify non-toxic substances that could sensitize cells to external electric fields. We found that local cationic anesthetics such as procaine, lidocaine and tetracaine greatly facilitated the electroporation of AT2 rat prostate carcinoma cells and human skin fibroblasts (HSF). This manifested as a 50% reduction in the strength of the electric field required to induce cell death by irreversible electroporation or to introduce fluorescent dyes such as calcein, carboxyfluorescein or Lucifer yellow into the cells. A similar decrease in the electric field thresholds for irreversible and reversible cell electroporation was observed when the cells were exposed to the electric field in the presence of the non-toxic cationic dyes 9-aminoacridine (9-AAA) or toluidine blue. Identifying non-toxic, reversibly acting cell sensitizers may facilitate cancer tissue ablation and help introduce therapeutic or diagnostic substances into the cells and tissuespl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biologii Komórkipl
dc.contributor.authorGrys, Maciejpl
dc.contributor.authorMadeja, Zbigniew - 130173 pl
dc.contributor.authorKorohoda, Włodzimierz - 129136 pl
dc.date.accessioned2015-04-27T09:56:30Z
dc.date.available2015-04-27T09:56:30Z
dc.date.issued2014pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. s. 74-76pl
dc.description.number1pl
dc.description.physical65-76pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume19pl
dc.identifier.doi10.2478/s11658-013-0114-zpl
dc.identifier.eissn1689-1392pl
dc.identifier.issn1425-8153pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/5809
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska*
dc.rights.licenceCC-BY-NC-ND
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/pl/legalcode*
dc.share.typeinne
dc.subject.enlocal cationic anestheticspl
dc.subject.enfocused electric fieldpl
dc.subject.endirect current electric fieldpl
dc.subject.enflow through an electric fieldpl
dc.subject.encell viabilitypl
dc.subject.enloading with fluorescent dyespl
dc.subject.enreversible electroporationpl
dc.subject.enirreversible electroporationpl
dc.subject.encationic dyespl
dc.subject.enselective sensitization of cellspl
dc.subtypeArticlepl
dc.titleDecreasing the thresholds for electroporation by sensitizing cells with local cationic anesthetics and substances that decrease the surface negative electric chargepl
dc.title.journalCellular & Molecular Biology Letterspl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T19:42:46Z
dc.abstract.enpl
The recently described method of cell electroporation by flow of cell suspension through localized direct current electric fields (dcEFs) was applied to identify non-toxic substances that could sensitize cells to external electric fields. We found that local cationic anesthetics such as procaine, lidocaine and tetracaine greatly facilitated the electroporation of AT2 rat prostate carcinoma cells and human skin fibroblasts (HSF). This manifested as a 50% reduction in the strength of the electric field required to induce cell death by irreversible electroporation or to introduce fluorescent dyes such as calcein, carboxyfluorescein or Lucifer yellow into the cells. A similar decrease in the electric field thresholds for irreversible and reversible cell electroporation was observed when the cells were exposed to the electric field in the presence of the non-toxic cationic dyes 9-aminoacridine (9-AAA) or toluidine blue. Identifying non-toxic, reversibly acting cell sensitizers may facilitate cancer tissue ablation and help introduce therapeutic or diagnostic substances into the cells and tissues
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biologii Komórki
dc.contributor.authorpl
Grys, Maciej
dc.contributor.authorpl
Madeja, Zbigniew - 130173
dc.contributor.authorpl
Korohoda, Włodzimierz - 129136
dc.date.accessioned
2015-04-27T09:56:30Z
dc.date.available
2015-04-27T09:56:30Z
dc.date.issuedpl
2014
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. s. 74-76
dc.description.numberpl
1
dc.description.physicalpl
65-76
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
19
dc.identifier.doipl
10.2478/s11658-013-0114-z
dc.identifier.eissnpl
1689-1392
dc.identifier.issnpl
1425-8153
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/5809
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska
dc.rights.licence
CC-BY-NC-ND
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-nd/3.0/pl/legalcode
dc.share.type
inne
dc.subject.enpl
local cationic anesthetics
dc.subject.enpl
focused electric field
dc.subject.enpl
direct current electric field
dc.subject.enpl
flow through an electric field
dc.subject.enpl
cell viability
dc.subject.enpl
loading with fluorescent dyes
dc.subject.enpl
reversible electroporation
dc.subject.enpl
irreversible electroporation
dc.subject.enpl
cationic dyes
dc.subject.enpl
selective sensitization of cells
dc.subtypepl
Article
dc.titlepl
Decreasing the thresholds for electroporation by sensitizing cells with local cationic anesthetics and substances that decrease the surface negative electric charge
dc.title.journalpl
Cellular & Molecular Biology Letters
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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