The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models

2021
journal article
article
6
cris.lastimport.wos2024-04-10T02:54:24Z
dc.abstract.enAlthough the key factor affecting the biocompatibility of IONPs is the core size, there is a lack of regular investigation concerning the impact of the parameter on the toxicity of these nanomaterials. Therefore, such studies were carried out in this paper. Their purpose was to compare the influence of PEG-coated-magnetite NPs with the core of 5, 10 and 30 nm on six carefully selected cell lines. The proliferation rate, viability, metabolic activity, migration activity, ROS levels and cytoskeleton architecture of cells have been evaluated for specified incubation periods. These were 24 and 72-h long incubations with IONPs administered in two doses: 5 and 25 mu g Fe/ml. A decrease in viability was observed after exposure to the tested NPs for all the analyzed cell lines. This effect was not connected with core diameter but depended on the exposure time to the nanomaterials. IONPs increased not only the proliferation rate of macrophages-being phagocytic cells-but also, under certain conditions stimulated tumor cell divisions. Most likely, the increase in proliferation rate of macrophages contributed to the changes in the architecture of their cytoskeleton. The growth in the level of ROS in cells had been induced mainly by the smallest NPs. This effect was observed for HEK293T cells and two cancerous lines: U87MG (at both doses tested) and T98G (only for the higher dose). This requires further study concerning both potential toxicity of such IONPs to the kidneys and assessing their therapeutic potential in the treatment of glioblastoma multiforme.pl
dc.affiliationWydział Biologii : Instytut Zoologii i Badań Biomedycznychpl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biologii Komórkipl
dc.contributor.authorJanik-Olchawa, Natalia - 226879 pl
dc.contributor.authorDrozdz, Agnieszkapl
dc.contributor.authorRyszawy, Damian - 104191 pl
dc.contributor.authorPudełek, Maciej - 258123 pl
dc.contributor.authorPlaneta, Karolinapl
dc.contributor.authorSetkowicz-Janeczko, Zuzanna - 131836 pl
dc.contributor.authorSniegocki, Maciejpl
dc.contributor.authorWytrwal-Sarna, Magdalenapl
dc.contributor.authorGajewska, Martapl
dc.contributor.authorChwiej, Joannapl
dc.date.accessioned2022-01-04T14:02:09Z
dc.date.available2022-01-04T14:02:09Z
dc.date.issued2021pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalW art. Zuzanna Setkowicz-Janeczko podpisana jako Zuzanna Setkowiczpl
dc.description.versionostateczna wersja wydawcy
dc.description.volume11pl
dc.identifier.articleid21808pl
dc.identifier.doi10.1038/s41598-021-01237-ypl
dc.identifier.eissn2045-2322pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/286529
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subtypeArticlepl
dc.titleThe influence of IONPs core size on their biocompatibility and activity in in vitro cellular modelspl
dc.title.journalScientific Reportspl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T02:54:24Z
dc.abstract.enpl
Although the key factor affecting the biocompatibility of IONPs is the core size, there is a lack of regular investigation concerning the impact of the parameter on the toxicity of these nanomaterials. Therefore, such studies were carried out in this paper. Their purpose was to compare the influence of PEG-coated-magnetite NPs with the core of 5, 10 and 30 nm on six carefully selected cell lines. The proliferation rate, viability, metabolic activity, migration activity, ROS levels and cytoskeleton architecture of cells have been evaluated for specified incubation periods. These were 24 and 72-h long incubations with IONPs administered in two doses: 5 and 25 mu g Fe/ml. A decrease in viability was observed after exposure to the tested NPs for all the analyzed cell lines. This effect was not connected with core diameter but depended on the exposure time to the nanomaterials. IONPs increased not only the proliferation rate of macrophages-being phagocytic cells-but also, under certain conditions stimulated tumor cell divisions. Most likely, the increase in proliferation rate of macrophages contributed to the changes in the architecture of their cytoskeleton. The growth in the level of ROS in cells had been induced mainly by the smallest NPs. This effect was observed for HEK293T cells and two cancerous lines: U87MG (at both doses tested) and T98G (only for the higher dose). This requires further study concerning both potential toxicity of such IONPs to the kidneys and assessing their therapeutic potential in the treatment of glioblastoma multiforme.
dc.affiliationpl
Wydział Biologii : Instytut Zoologii i Badań Biomedycznych
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biologii Komórki
dc.contributor.authorpl
Janik-Olchawa, Natalia - 226879
dc.contributor.authorpl
Drozdz, Agnieszka
dc.contributor.authorpl
Ryszawy, Damian - 104191
dc.contributor.authorpl
Pudełek, Maciej - 258123
dc.contributor.authorpl
Planeta, Karolina
dc.contributor.authorpl
Setkowicz-Janeczko, Zuzanna - 131836
dc.contributor.authorpl
Sniegocki, Maciej
dc.contributor.authorpl
Wytrwal-Sarna, Magdalena
dc.contributor.authorpl
Gajewska, Marta
dc.contributor.authorpl
Chwiej, Joanna
dc.date.accessioned
2022-01-04T14:02:09Z
dc.date.available
2022-01-04T14:02:09Z
dc.date.issuedpl
2021
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
W art. Zuzanna Setkowicz-Janeczko podpisana jako Zuzanna Setkowicz
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
11
dc.identifier.articleidpl
21808
dc.identifier.doipl
10.1038/s41598-021-01237-y
dc.identifier.eissnpl
2045-2322
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/286529
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models
dc.title.journalpl
Scientific Reports
dc.typepl
JournalArticle
dspace.entity.type
Publication

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