The effect of TCDD dioxin on the rat liver in biochemical and histological assessment

2010
journal article
article
20
dc.abstract.enEighteen male Wistar rats were divided into 3 groups of 6 animals each. Two groups received different intraperitoneal doses of TCDD (0.75 and 8 μg) in DMSO solution and the third group (control) received only DMSO on days 0, 7 and 14. On day 21 the animals were sacrificed, and then blood tests, pathological examination and CYP1A1 activity measurement were performed. In rats that received a high dose of dioxin (8 μg) hepatic lobules revealed parenchymal degeneration and vacuolization of hepatocytes was observed, and also an increased CYP reaction was found in central parts of lobules, around the central vein. The reaction in control and low dose groups was weak. The resorufin level was significantly (P<0.05) higher in the group receiving a low dose of dioxin as compared to the control group. The study confirmed that TCDD damages the rat liver in a dose-dependent manner. Administration of high TCDD doses causing major liver damage also damaged CYP1A1 (based on higher resorufin levels in epiluminescence). TCDD activates CYP1A1, which was confirmed by increased immunohistochemical reactivity of central areas of hepatic lobules.pl
dc.affiliationWydział Lekarski : Klinika Gastroenterologii i Hepatologiipl
dc.affiliationWydział Lekarski : Zakład Patomorfologii Klinicznej i Doświadczalnejpl
dc.affiliationWydział Lekarski : Zakład Histologiipl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorCzepiel, Jacek - 129104 pl
dc.contributor.authorBiesiada, Grażyna - 128770 pl
dc.contributor.authorGajda, Mariusz - 129426 pl
dc.contributor.authorSzczepański, Wojciech - 133571 pl
dc.contributor.authorSzypuła, Kingapl
dc.contributor.authorDąbrowski, Zbigniewpl
dc.contributor.authorMach, Tomasz - 130768 pl
dc.date.accession2020-07-10pl
dc.date.accessioned2020-07-10T13:17:06Z
dc.date.available2020-07-10T13:17:06Z
dc.date.issued2010pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. s. 90pl
dc.description.number1-2pl
dc.description.physical85-90pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume58pl
dc.identifier.doi10.3409/fb58_1-2.85-90pl
dc.identifier.eissn1734-9168pl
dc.identifier.issn0015-5497pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/165689
dc.identifier.weblinkhttp://www.isez.pan.krakow.pl/journals/folia/pdf/58(1-2)/58(1-2)_14.pdfpl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enrat pathologypl
dc.subject.enCYP1A1pl
dc.subject.enimmunohistochemistrypl
dc.subject.enliverpl
dc.subject.enTCDDpl
dc.subtypeArticlepl
dc.titleThe effect of TCDD dioxin on the rat liver in biochemical and histological assessmentpl
dc.title.journalFolia Biologicapl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Eighteen male Wistar rats were divided into 3 groups of 6 animals each. Two groups received different intraperitoneal doses of TCDD (0.75 and 8 μg) in DMSO solution and the third group (control) received only DMSO on days 0, 7 and 14. On day 21 the animals were sacrificed, and then blood tests, pathological examination and CYP1A1 activity measurement were performed. In rats that received a high dose of dioxin (8 μg) hepatic lobules revealed parenchymal degeneration and vacuolization of hepatocytes was observed, and also an increased CYP reaction was found in central parts of lobules, around the central vein. The reaction in control and low dose groups was weak. The resorufin level was significantly (P<0.05) higher in the group receiving a low dose of dioxin as compared to the control group. The study confirmed that TCDD damages the rat liver in a dose-dependent manner. Administration of high TCDD doses causing major liver damage also damaged CYP1A1 (based on higher resorufin levels in epiluminescence). TCDD activates CYP1A1, which was confirmed by increased immunohistochemical reactivity of central areas of hepatic lobules.
dc.affiliationpl
Wydział Lekarski : Klinika Gastroenterologii i Hepatologii
dc.affiliationpl
Wydział Lekarski : Zakład Patomorfologii Klinicznej i Doświadczalnej
dc.affiliationpl
Wydział Lekarski : Zakład Histologii
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Czepiel, Jacek - 129104
dc.contributor.authorpl
Biesiada, Grażyna - 128770
dc.contributor.authorpl
Gajda, Mariusz - 129426
dc.contributor.authorpl
Szczepański, Wojciech - 133571
dc.contributor.authorpl
Szypuła, Kinga
dc.contributor.authorpl
Dąbrowski, Zbigniew
dc.contributor.authorpl
Mach, Tomasz - 130768
dc.date.accessionpl
2020-07-10
dc.date.accessioned
2020-07-10T13:17:06Z
dc.date.available
2020-07-10T13:17:06Z
dc.date.issuedpl
2010
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. s. 90
dc.description.numberpl
1-2
dc.description.physicalpl
85-90
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
58
dc.identifier.doipl
10.3409/fb58_1-2.85-90
dc.identifier.eissnpl
1734-9168
dc.identifier.issnpl
0015-5497
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/165689
dc.identifier.weblinkpl
http://www.isez.pan.krakow.pl/journals/folia/pdf/58(1-2)/58(1-2)_14.pdf
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
rat pathology
dc.subject.enpl
CYP1A1
dc.subject.enpl
immunohistochemistry
dc.subject.enpl
liver
dc.subject.enpl
TCDD
dc.subtypepl
Article
dc.titlepl
The effect of TCDD dioxin on the rat liver in biochemical and histological assessment
dc.title.journalpl
Folia Biologica
dc.typepl
JournalArticle
dspace.entity.type
Publication

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