MRI-based in vivo detection of coronary microvascular dysfunction before alterations in cardiac function induced by short-term high-fat diet in mice

2021
journal article
article
8
dc.abstract.enEndothelial dysfunction is one of the hallmarks of vascular abnormalities in metabolic diseases and has been repeatedly demonstrated in coronary and peripheral circulation in mice fed high-fat diet (HFD), particularly after long-term HFD. However, the temporal relationship between development of coronary microvascular endothelial dysfunction and deterioration in diastolic and systolic cardiac function after short-term feeding with HFD has not yet been studied. This study aimed to correlate the changes in coronary microvascular endothelial function and global cardiac performance indices in vivo after short-term feeding with HFD in mice. Short-term feeding with a HFD (60% fat + 1% cholesterol) resulted in severely impaired coronary microvascular function, as evidenced by the diminished effect of nitric oxide synthase inhibition (by L-NAME) assessed using T1 mapping via in vivo MRI. Deterioration of coronary microvascular function was detected as early as after 7 days of HFD and further declined after 8 weeks on a HFD. HFD-induced coronary microvascular dysfunction was not associated with impaired myocardial capillary density and was present before systemic insulin resistance assessed by a glucose tolerance test. Basal coronary flow and coronary reserve, as assessed using the A2A adenosine receptor agonist regadenoson, were also not altered in HFD-fed mice. Histological analysis did not reveal cardiomyocyte hypertrophy or fibrosis. Increased lipid accumulation in cardiomyocytes was detected as early as after 7 days of HFD and remained at a similar level at 8 weeks on a HFD. Multiparametric cardiac MRI revealed a reduction in systolic heart function, including decreased ejection rate, increased end-systolic volume and decreased myocardial strain in diastole with impaired ejection fraction, but not until 4 weeks of HFD. Short-term feeding with HFD resulted in early endothelial dysfunction in coronary microcirculation that preceded alteration in cardiac function and systemic insulin resistance.pl
dc.affiliationPion Prorektora ds. badań naukowych : Jagiellońskie Centrum Rozwoju Lekówpl
dc.cm.id105933pl
dc.cm.idOmegaUJCM3212965743384e72a2e420c73be4bbaapl
dc.contributor.authorKwiatkowski, Grzegorz - 149211 pl
dc.contributor.authorBar, Anna - 163430 pl
dc.contributor.authorJasztal, Agnieszka - 129875 pl
dc.contributor.authorChłopicki, Stefan - 128995 pl
dc.date.accession2022-02-01pl
dc.date.accessioned2021-10-07T07:33:15Z
dc.date.available2021-10-07T07:33:15Z
dc.date.issued2021pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number1pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume11pl
dc.identifier.articleid18915pl
dc.identifier.doi10.1038/s41598-021-98401-1pl
dc.identifier.eissn2045-2322pl
dc.identifier.issn2045-2322pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/279597
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-021-98401-1pl
dc.languageengpl
dc.language.containerengpl
dc.pbn.affiliationDziedzina nauk medycznych i nauk o zdrowiu : nauki medyczne
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeOtwarte czasopismo
dc.subtypeArticlepl
dc.titleMRI-based in vivo detection of coronary microvascular dysfunction before alterations in cardiac function induced by short-term high-fat diet in micepl
dc.title.journalScientific Reportspl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Endothelial dysfunction is one of the hallmarks of vascular abnormalities in metabolic diseases and has been repeatedly demonstrated in coronary and peripheral circulation in mice fed high-fat diet (HFD), particularly after long-term HFD. However, the temporal relationship between development of coronary microvascular endothelial dysfunction and deterioration in diastolic and systolic cardiac function after short-term feeding with HFD has not yet been studied. This study aimed to correlate the changes in coronary microvascular endothelial function and global cardiac performance indices in vivo after short-term feeding with HFD in mice. Short-term feeding with a HFD (60% fat + 1% cholesterol) resulted in severely impaired coronary microvascular function, as evidenced by the diminished effect of nitric oxide synthase inhibition (by L-NAME) assessed using T1 mapping via in vivo MRI. Deterioration of coronary microvascular function was detected as early as after 7 days of HFD and further declined after 8 weeks on a HFD. HFD-induced coronary microvascular dysfunction was not associated with impaired myocardial capillary density and was present before systemic insulin resistance assessed by a glucose tolerance test. Basal coronary flow and coronary reserve, as assessed using the A2A adenosine receptor agonist regadenoson, were also not altered in HFD-fed mice. Histological analysis did not reveal cardiomyocyte hypertrophy or fibrosis. Increased lipid accumulation in cardiomyocytes was detected as early as after 7 days of HFD and remained at a similar level at 8 weeks on a HFD. Multiparametric cardiac MRI revealed a reduction in systolic heart function, including decreased ejection rate, increased end-systolic volume and decreased myocardial strain in diastole with impaired ejection fraction, but not until 4 weeks of HFD. Short-term feeding with HFD resulted in early endothelial dysfunction in coronary microcirculation that preceded alteration in cardiac function and systemic insulin resistance.
dc.affiliationpl
Pion Prorektora ds. badań naukowych : Jagiellońskie Centrum Rozwoju Leków
dc.cm.idpl
105933
dc.cm.idOmegapl
UJCM3212965743384e72a2e420c73be4bbaa
dc.contributor.authorpl
Kwiatkowski, Grzegorz - 149211
dc.contributor.authorpl
Bar, Anna - 163430
dc.contributor.authorpl
Jasztal, Agnieszka - 129875
dc.contributor.authorpl
Chłopicki, Stefan - 128995
dc.date.accessionpl
2022-02-01
dc.date.accessioned
2021-10-07T07:33:15Z
dc.date.available
2021-10-07T07:33:15Z
dc.date.issuedpl
2021
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
1
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
11
dc.identifier.articleidpl
18915
dc.identifier.doipl
10.1038/s41598-021-98401-1
dc.identifier.eissnpl
2045-2322
dc.identifier.issnpl
2045-2322
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/279597
dc.identifier.weblinkpl
https://www.nature.com/articles/s41598-021-98401-1
dc.languagepl
eng
dc.language.containerpl
eng
dc.pbn.affiliation
Dziedzina nauk medycznych i nauk o zdrowiu : nauki medyczne
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
Otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
MRI-based in vivo detection of coronary microvascular dysfunction before alterations in cardiac function induced by short-term high-fat diet in mice
dc.title.journalpl
Scientific Reports
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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