miR-378 influences muscle satellite cells and enhances adipogenic potential of fibro-adipogenic progenitors but does not affect muscle regeneration in the glycerol-induced injury model

2023
journal article
article
2
dc.abstract.enSkeletal muscle regeneration relies on the reciprocal interaction between many types of cells. Regenerative capacity may be altered in different disorders. In our study, we investigated whether the deletion of miR-378a (miR-378) affects muscle regeneration. We subjected 6-week-old wild-type (WT) and miR-378 knockout (miR-378$^{–/–}$) animals to the glycerol-induced muscle injury and performed analyses in various time-points. In miR-378$^{–/–}$ animals, an elevated abundance of muscle satellite cells (mSCs) on day 3 was found. Furthermore, fibro-adipogenic progenitors (FAPs) isolated from the muscle of miR-378$^{–/–}$ mice exhibited enhanced adipogenic potential. At the same time, lack of miR-378 did not affect inflammation, fibrosis, adipose tissue deposition, centrally nucleated fiber count, muscle fiber size, FAP abundance, and muscle contractility at any time point analyzed. To conclude, our study revealed that miR-378 deletion influences the abundance of mSCs and the adipogenic potential of FAPs, but does not affect overall regeneration upon acute, glycerol-induced muscle injury.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Medycznejpl
dc.affiliationPion Prorektora ds. badań naukowych : Małopolskie Centrum Biotechnologiipl
dc.contributor.authorMucha, Olga - 206879 pl
dc.contributor.authorPodkalicka, Paulina - 201153 pl
dc.contributor.authorŻukowska, Monika - 182004 pl
dc.contributor.authorPośpiech, Ewelina - 108809 pl
dc.contributor.authorDulak, Józef - 127818 pl
dc.contributor.authorŁoboda, Agnieszka - 130081 pl
dc.date.accessioned2023-08-21T10:21:02Z
dc.date.available2023-08-21T10:21:02Z
dc.date.issued2023pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.versionostateczna wersja wydawcy
dc.description.volume13pl
dc.identifier.articleid13434pl
dc.identifier.doi10.1038/s41598-023-40729-xpl
dc.identifier.eissn2045-2322pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/317823
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subtypeArticlepl
dc.titlemiR-378 influences muscle satellite cells and enhances adipogenic potential of fibro-adipogenic progenitors but does not affect muscle regeneration in the glycerol-induced injury modelpl
dc.title.journalScientific Reportspl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Skeletal muscle regeneration relies on the reciprocal interaction between many types of cells. Regenerative capacity may be altered in different disorders. In our study, we investigated whether the deletion of miR-378a (miR-378) affects muscle regeneration. We subjected 6-week-old wild-type (WT) and miR-378 knockout (miR-378$^{–/–}$) animals to the glycerol-induced muscle injury and performed analyses in various time-points. In miR-378$^{–/–}$ animals, an elevated abundance of muscle satellite cells (mSCs) on day 3 was found. Furthermore, fibro-adipogenic progenitors (FAPs) isolated from the muscle of miR-378$^{–/–}$ mice exhibited enhanced adipogenic potential. At the same time, lack of miR-378 did not affect inflammation, fibrosis, adipose tissue deposition, centrally nucleated fiber count, muscle fiber size, FAP abundance, and muscle contractility at any time point analyzed. To conclude, our study revealed that miR-378 deletion influences the abundance of mSCs and the adipogenic potential of FAPs, but does not affect overall regeneration upon acute, glycerol-induced muscle injury.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Medycznej
dc.affiliationpl
Pion Prorektora ds. badań naukowych : Małopolskie Centrum Biotechnologii
dc.contributor.authorpl
Mucha, Olga - 206879
dc.contributor.authorpl
Podkalicka, Paulina - 201153
dc.contributor.authorpl
Żukowska, Monika - 182004
dc.contributor.authorpl
Pośpiech, Ewelina - 108809
dc.contributor.authorpl
Dulak, Józef - 127818
dc.contributor.authorpl
Łoboda, Agnieszka - 130081
dc.date.accessioned
2023-08-21T10:21:02Z
dc.date.available
2023-08-21T10:21:02Z
dc.date.issuedpl
2023
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
13
dc.identifier.articleidpl
13434
dc.identifier.doipl
10.1038/s41598-023-40729-x
dc.identifier.eissnpl
2045-2322
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/317823
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
miR-378 influences muscle satellite cells and enhances adipogenic potential of fibro-adipogenic progenitors but does not affect muscle regeneration in the glycerol-induced injury model
dc.title.journalpl
Scientific Reports
dc.typepl
JournalArticle
dspace.entity.type
Publication

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