Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts

2015
journal article
article
48
dc.abstract.enBackground : Candida parapsilosis and C. tropicalis increasingly compete with C. albicans—the most common fungal pathogen in humans—as causative agents of severe candidiasis in immunocompromised patients. In contrast to C. albicans, the pathogenic mechanisms of these two non-albicans Candida species are poorly understood. Adhesion of Candida yeast to host cells and the extracellular matrix is critical for fungal invasion of hosts. Methods : The fungal proteins involved in interactions with extracellular matrix proteins were isolated from mixtures of β-1,3-glucanase– or β-1,6-glucanase–extractable cell wall-associated proteins by use of affinity chromatography and chemical cross-linking methods, and were further identified by liquid chromatography-coupled tandem mass spectrometry. Results : In the present study, we characterized the binding of three major extracellular matrix proteins—fibronectin, vitronectin and laminin—to C. parapsilosis and C. tropicalis pseudohyphae. The major individual compounds of the fungal cell wall that bound fibronectin, vitronectin and laminin were found to comprise two groups: (1) true cell wall components similar to C. albicans adhesins from the Als, Hwp and Iff/Hyr families; and (2) atypical (cytoplasm-derived) surface-exposed proteins, including malate synthase, glucose-6-phosphate isomerase, 6-phosphogluconate dehydrogenase, enolase, fructose-1,6-bisphosphatase, transketolase, transaldolase and elongation factor 2. Discussion : The adhesive abilities of two investigated non-albicans Candida species toward extracellular matrix proteins were comparable to those of C. albicans suggesting an important role of this particular virulence attribute in the pathogenesis of infections caused by C. tropicalis and C. parapsilosis. Conclusions : Our results reveal new insight into host–pathogen interactions during infections by two important, recently emerging, fungal pathogens.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznejpl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznejpl
dc.affiliationPion Prorektora ds. badań naukowych i funduszy strukturalnych : Małopolskie Centrum Biotechnologiipl
dc.contributor.authorKozik, Andrzej - 129351 pl
dc.contributor.authorKarkowska-Kuleta, Justyna - 104458 pl
dc.contributor.authorSatała, Dorota - 177568 pl
dc.contributor.authorBocheńska, Oliwia - 173990 pl
dc.contributor.authorKędracka-Krok, Sylwia - 128739 pl
dc.contributor.authorJankowska, Urszula - 103969 pl
dc.contributor.authorRąpała-Kozik, Maria - 131641 pl
dc.date.accessioned2015-11-03T11:54:18Z
dc.date.available2015-11-03T11:54:18Z
dc.date.issued2015pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalDorota Satała podpisana nazwiskiem Zającpl
dc.description.versionostateczna wersja wydawcy
dc.description.volume15pl
dc.identifier.articleid197pl
dc.identifier.doi10.1186/s12866-015-0531-4pl
dc.identifier.eissn1471-2180pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/16480
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.ennon-albicans Candida speciespl
dc.subject.enextracellular matrixpl
dc.subject.encandidiasispl
dc.subject.enfibronectinpl
dc.subject.envitronectinpl
dc.subject.enlamininpl
dc.subtypeArticlepl
dc.titleFibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeastspl
dc.title.journalBMC Microbiologypl
dc.title.volumeOctoberpl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Background : Candida parapsilosis and C. tropicalis increasingly compete with C. albicans—the most common fungal pathogen in humans—as causative agents of severe candidiasis in immunocompromised patients. In contrast to C. albicans, the pathogenic mechanisms of these two non-albicans Candida species are poorly understood. Adhesion of Candida yeast to host cells and the extracellular matrix is critical for fungal invasion of hosts. Methods : The fungal proteins involved in interactions with extracellular matrix proteins were isolated from mixtures of β-1,3-glucanase– or β-1,6-glucanase–extractable cell wall-associated proteins by use of affinity chromatography and chemical cross-linking methods, and were further identified by liquid chromatography-coupled tandem mass spectrometry. Results : In the present study, we characterized the binding of three major extracellular matrix proteins—fibronectin, vitronectin and laminin—to C. parapsilosis and C. tropicalis pseudohyphae. The major individual compounds of the fungal cell wall that bound fibronectin, vitronectin and laminin were found to comprise two groups: (1) true cell wall components similar to C. albicans adhesins from the Als, Hwp and Iff/Hyr families; and (2) atypical (cytoplasm-derived) surface-exposed proteins, including malate synthase, glucose-6-phosphate isomerase, 6-phosphogluconate dehydrogenase, enolase, fructose-1,6-bisphosphatase, transketolase, transaldolase and elongation factor 2. Discussion : The adhesive abilities of two investigated non-albicans Candida species toward extracellular matrix proteins were comparable to those of C. albicans suggesting an important role of this particular virulence attribute in the pathogenesis of infections caused by C. tropicalis and C. parapsilosis. Conclusions : Our results reveal new insight into host–pathogen interactions during infections by two important, recently emerging, fungal pathogens.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznej
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej
dc.affiliationpl
Pion Prorektora ds. badań naukowych i funduszy strukturalnych : Małopolskie Centrum Biotechnologii
dc.contributor.authorpl
Kozik, Andrzej - 129351
dc.contributor.authorpl
Karkowska-Kuleta, Justyna - 104458
dc.contributor.authorpl
Satała, Dorota - 177568
dc.contributor.authorpl
Bocheńska, Oliwia - 173990
dc.contributor.authorpl
Kędracka-Krok, Sylwia - 128739
dc.contributor.authorpl
Jankowska, Urszula - 103969
dc.contributor.authorpl
Rąpała-Kozik, Maria - 131641
dc.date.accessioned
2015-11-03T11:54:18Z
dc.date.available
2015-11-03T11:54:18Z
dc.date.issuedpl
2015
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Dorota Satała podpisana nazwiskiem Zając
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
15
dc.identifier.articleidpl
197
dc.identifier.doipl
10.1186/s12866-015-0531-4
dc.identifier.eissnpl
1471-2180
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/16480
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
non-albicans Candida species
dc.subject.enpl
extracellular matrix
dc.subject.enpl
candidiasis
dc.subject.enpl
fibronectin
dc.subject.enpl
vitronectin
dc.subject.enpl
laminin
dc.subtypepl
Article
dc.titlepl
Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts
dc.title.journalpl
BMC Microbiology
dc.title.volumepl
October
dc.typepl
JournalArticle
dspace.entity.type
Publication

* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.

Views
12
Views per month
Views per city
Ashburn
3
Wroclaw
2
Chandler
1
Des Moines
1
Dublin
1
Krakow
1
Downloads
kozik_karkowska-kuleta_zajac_bochenska_kedracka-krok_jankowska_rapala-kozik_fibronectin-_vitronectin-_and_laminin-binding_proteins_2015.pdf
4
kozik_karkowska-kuleta_zajac_bochenska_kedracka-krok_jankowska_rapala-kozik_fibronectin-_vitronectin-_and_laminin-binding_proteins_2015.odt
2