Sugar and light effects on the condition of the photosynthetic apparatus of Arabidopsis thaliana cultured in vitro

2012
journal article
article
64
dc.abstract.enLight and sugars are fundamental elements of plant metabolism and play signaling roles in many processes. They are also critical factors determining the condition of plants cultured in vitro. The aim of this work was to investigate the simultaneous influence of irradiance and sugar content in the medium on the growth and photosynthetic apparatus condition of Arabidopsis thaliana in vitro. Plants were grown on media containing 1 or 3% of sucrose or glucose at three irradiances: 25, 100, and 250 μmol m^{−2} s^{−1} (weak, medium, and strong light). Media without sugar were used for control plants. Plant growth parameters were measured and the following physiological processes were investigated: photosynthesis, blue light-induced chloroplast relocations, and xanthophyll cycle activity. The expression of genes related to these processes was analyzed. The presence of sugar in the medium was found to be essential for the growth of Arabidopsis in vitro. Weak light significantly limited growth and the capacity to acclimate to changing light conditions. Strong light was a source of stress in some cases. Contrary to earlier reports, exogenous sugars showed a positive effect on photosynthesis. At higher concentration they acted as photoprotectants, overcoming the negative influence of strong light on photosynthesis and the xanthophyll cycle. The expression of all investigated genes was influenced by irradiance and sugar presence. In many cases differential effects of sugar type and concentration could be observed. The specific effects of some irradiance/sugar concentration combinations point to possible interactions between sugar- and light-induced signaling pathways.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Roślinpl
dc.contributor.authorEckstein, Aleksandra - 103907 pl
dc.contributor.authorZięba, Patrycjapl
dc.contributor.authorGabryś, Halina - 127971 pl
dc.date.accessioned2016-05-16T11:03:38Z
dc.date.available2016-05-16T11:03:38Z
dc.date.issued2012pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr.s. 100-101pl
dc.description.number1pl
dc.description.physical90-101pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume31pl
dc.identifier.doi10.1007/s00344-011-9222-zpl
dc.identifier.eissn1435-8107pl
dc.identifier.issn0721-7595pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/26041
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceCC-BY-NC
dc.rights.uri*
dc.share.typeinne
dc.subject.enArabidopsis thalianapl
dc.subject.enlightpl
dc.subject.ensugarspl
dc.subject.englucosepl
dc.subject.ensucrosepl
dc.subject.enin vitro culturepl
dc.subject.enphotosynthesispl
dc.subject.enchloroplast movementspl
dc.subject.enxanthophyll cyclepl
dc.subtypeArticlepl
dc.titleSugar and light effects on the condition of the photosynthetic apparatus of Arabidopsis thaliana cultured in vitropl
dc.title.journalJournal of Plant Growth Regulationpl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Light and sugars are fundamental elements of plant metabolism and play signaling roles in many processes. They are also critical factors determining the condition of plants cultured in vitro. The aim of this work was to investigate the simultaneous influence of irradiance and sugar content in the medium on the growth and photosynthetic apparatus condition of Arabidopsis thaliana in vitro. Plants were grown on media containing 1 or 3% of sucrose or glucose at three irradiances: 25, 100, and 250 μmol m^{−2} s^{−1} (weak, medium, and strong light). Media without sugar were used for control plants. Plant growth parameters were measured and the following physiological processes were investigated: photosynthesis, blue light-induced chloroplast relocations, and xanthophyll cycle activity. The expression of genes related to these processes was analyzed. The presence of sugar in the medium was found to be essential for the growth of Arabidopsis in vitro. Weak light significantly limited growth and the capacity to acclimate to changing light conditions. Strong light was a source of stress in some cases. Contrary to earlier reports, exogenous sugars showed a positive effect on photosynthesis. At higher concentration they acted as photoprotectants, overcoming the negative influence of strong light on photosynthesis and the xanthophyll cycle. The expression of all investigated genes was influenced by irradiance and sugar presence. In many cases differential effects of sugar type and concentration could be observed. The specific effects of some irradiance/sugar concentration combinations point to possible interactions between sugar- and light-induced signaling pathways.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Roślin
dc.contributor.authorpl
Eckstein, Aleksandra - 103907
dc.contributor.authorpl
Zięba, Patrycja
dc.contributor.authorpl
Gabryś, Halina - 127971
dc.date.accessioned
2016-05-16T11:03:38Z
dc.date.available
2016-05-16T11:03:38Z
dc.date.issuedpl
2012
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr.s. 100-101
dc.description.numberpl
1
dc.description.physicalpl
90-101
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
31
dc.identifier.doipl
10.1007/s00344-011-9222-z
dc.identifier.eissnpl
1435-8107
dc.identifier.issnpl
0721-7595
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/26041
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
CC-BY-NC
dc.rights.uri*
dc.share.type
inne
dc.subject.enpl
Arabidopsis thaliana
dc.subject.enpl
light
dc.subject.enpl
sugars
dc.subject.enpl
glucose
dc.subject.enpl
sucrose
dc.subject.enpl
in vitro culture
dc.subject.enpl
photosynthesis
dc.subject.enpl
chloroplast movements
dc.subject.enpl
xanthophyll cycle
dc.subtypepl
Article
dc.titlepl
Sugar and light effects on the condition of the photosynthetic apparatus of Arabidopsis thaliana cultured in vitro
dc.title.journalpl
Journal of Plant Growth Regulation
dc.typepl
JournalArticle
dspace.entity.type
Publication

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