In vitro testing the potential of a novel chimeric IgG variant for inhibiting collagen fibrils formation in recurrent hereditary gingival fibromatosis: chimeric antibody in a gingival model

2014
journal article
article
dc.abstract.enGingival fibromatosis is a progressive enlargement of the gingiva. It may hinder oral cavity hygiene and result in underlying bone loss. The long-term benefits of surgery cannot be predicted. On the other hand, alternative, efficient and non-invasive methods are not available at present. The aim of this study was to test the inhibitory effects of a chimeric IgG variant on collagen fibril formation in the cell culture of gingival fibroblasts taken from a patient with hereditary gingival fibromatosis with a high propensity for recurrence. Gingival biopsies were collected from the mandibular gingiva and used for histological evaluation as well as to establish a fibroblast culture. A histological evaluation was made in haematoxylin-eosin and Heidenhain's trichrome stained tissue sections. The inhibitory effect of a chimeric antibody on collagen fibril formation was determined in fibroblast cultures by using a collagen-specific Western blot and immunofluorescent staining. A histological evaluation revealed epithelial acanthosis with singular elongated rete pegs extending into the underlying connective tissue stroma that consisted of locally abundant, irregular collagen bundles. Based on observations with an in vitro model we conclude that a chimeric anti-collagen antibody efficiently inhibits collagen fibril accumulation in cell culture derived from diffuse, hereditary gingival fibromatosis that is characterized by a high propensity for recurrence (high proliferation index). Employing cell cultures from standardized group of patients with recurrent hereditary gingival fibromatosis as well as standarizing relevant 3D (tissue-like) models will be crucial for further tests of the antibody.pl
dc.affiliationWydział Lekarski : Instytut Stomatologiipl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Mikrobiologiipl
dc.cm.id67199
dc.contributor.authorGawron, Katarzyna - 200071 pl
dc.contributor.authorŁazarz-Bartyzel, Katarzyna - 173934 pl
dc.contributor.authorŁazarz, M.pl
dc.contributor.authorSteplewska, K.pl
dc.contributor.authorPyrć, Krzysztof - 161470 pl
dc.contributor.authorPotempa, Jan - 131531 pl
dc.contributor.authorChomyszyn-Gajewska, Maria - 129012 pl
dc.date.accession2015-04-29pl
dc.date.accessioned2015-05-08T10:44:55Z
dc.date.available2015-05-08T10:44:55Z
dc.date.issued2014pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number4pl
dc.description.physical585-591pl
dc.description.points25pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume65pl
dc.identifier.eissn1899-1505pl
dc.identifier.issn0867-5910pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/6768
dc.identifier.weblinkhttp://www.jpp.krakow.pl/journal/archive/08_14/pdf/585_08_14_article.pdfpl
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceOTHER
dc.rights.uri*
dc.share.typeotwarte czasopismo
dc.subject.enhereditary gingival fibromatosispl
dc.subject.enchimeric antibodypl
dc.subject.encollagen fibrilspl
dc.subject.enrecurrencepl
dc.subtypeArticlepl
dc.titleIn vitro testing the potential of a novel chimeric IgG variant for inhibiting collagen fibrils formation in recurrent hereditary gingival fibromatosis: chimeric antibody in a gingival modelpl
dc.title.journalJournal of Physiology and Pharmacologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Gingival fibromatosis is a progressive enlargement of the gingiva. It may hinder oral cavity hygiene and result in underlying bone loss. The long-term benefits of surgery cannot be predicted. On the other hand, alternative, efficient and non-invasive methods are not available at present. The aim of this study was to test the inhibitory effects of a chimeric IgG variant on collagen fibril formation in the cell culture of gingival fibroblasts taken from a patient with hereditary gingival fibromatosis with a high propensity for recurrence. Gingival biopsies were collected from the mandibular gingiva and used for histological evaluation as well as to establish a fibroblast culture. A histological evaluation was made in haematoxylin-eosin and Heidenhain's trichrome stained tissue sections. The inhibitory effect of a chimeric antibody on collagen fibril formation was determined in fibroblast cultures by using a collagen-specific Western blot and immunofluorescent staining. A histological evaluation revealed epithelial acanthosis with singular elongated rete pegs extending into the underlying connective tissue stroma that consisted of locally abundant, irregular collagen bundles. Based on observations with an in vitro model we conclude that a chimeric anti-collagen antibody efficiently inhibits collagen fibril accumulation in cell culture derived from diffuse, hereditary gingival fibromatosis that is characterized by a high propensity for recurrence (high proliferation index). Employing cell cultures from standardized group of patients with recurrent hereditary gingival fibromatosis as well as standarizing relevant 3D (tissue-like) models will be crucial for further tests of the antibody.
dc.affiliationpl
Wydział Lekarski : Instytut Stomatologii
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Mikrobiologii
dc.cm.id
67199
dc.contributor.authorpl
Gawron, Katarzyna - 200071
dc.contributor.authorpl
Łazarz-Bartyzel, Katarzyna - 173934
dc.contributor.authorpl
Łazarz, M.
dc.contributor.authorpl
Steplewska, K.
dc.contributor.authorpl
Pyrć, Krzysztof - 161470
dc.contributor.authorpl
Potempa, Jan - 131531
dc.contributor.authorpl
Chomyszyn-Gajewska, Maria - 129012
dc.date.accessionpl
2015-04-29
dc.date.accessioned
2015-05-08T10:44:55Z
dc.date.available
2015-05-08T10:44:55Z
dc.date.issuedpl
2014
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
4
dc.description.physicalpl
585-591
dc.description.pointspl
25
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
65
dc.identifier.eissnpl
1899-1505
dc.identifier.issnpl
0867-5910
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/6768
dc.identifier.weblinkpl
http://www.jpp.krakow.pl/journal/archive/08_14/pdf/585_08_14_article.pdf
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
OTHER
dc.rights.uri*
dc.share.type
otwarte czasopismo
dc.subject.enpl
hereditary gingival fibromatosis
dc.subject.enpl
chimeric antibody
dc.subject.enpl
collagen fibrils
dc.subject.enpl
recurrence
dc.subtypepl
Article
dc.titlepl
In vitro testing the potential of a novel chimeric IgG variant for inhibiting collagen fibrils formation in recurrent hereditary gingival fibromatosis: chimeric antibody in a gingival model
dc.title.journalpl
Journal of Physiology and Pharmacology
dc.typepl
JournalArticle
dspace.entity.type
Publication

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