This paper presents an electrochemical approach to quantitative and qualitative determination of hordenine (HODE) at the electrified liquid-liquid interface (eLLI). In this regard, we have employed ion transfer voltammetry (ITV) as an electroanalytical detection technique. The response of peak current values (positive or negative currents) increased linearly with HODE concentration in the studied concentration range from 28.49 to 1250 μM. Furthermore, the effect of pH (2-12) of the aqueous phase on recorded HODE signals was studied. Based on the obtained results a number of significant physicochemical parameters, such as the formal Galvani potential of the ion transfer reaction , diffusion coefficients (D), formal free Gibbs energy of the ion transfer reaction and water-1,2-dichloroethane partition coefficient were determined. Finally, we have also showed that the elaborated method can be successfully used in the electroanalysis of complex matrixes, especially: beer and bodybuilding supplement samples.