Postnatal exposure to flutamide affects CDH1 and CTNNB1 gene expression in adult pig epididymis and prostate and alters metabolism of testosterone

2014
journal article
article
19
cris.lastimport.wos2024-04-09T22:41:24Z
dc.abstract.otherIn both epididymis and prostate the dynamic cross-talk between the cells is hormonally regulated and, in part, through direct cell-to-cell interactions. Functionality of the male reproductive organs may be affected by exposure to specific chemicals, so-called ‘reprotoxicants’. In this study we tested whether early postnatal and prepubertal exposure to anti-androgen flutamide altered the expression of adherens junction genes encoding E-cadherin (CDH1) and β-catenin (CTNNB1) in adult pig epididymis and prostate. In addition, the expression of mRNAs and proteins for 5α-reductase (ST5AR2) and aromatase (CYP19A1) were examined to show whether flutamide alters metabolism of testosterone. Thus, flutamide was injected into male piglets between Days 2 and 10 and between Days 90 and 98 postnatally (PD2 and PD90; 50 mg/kg bw), tissues that were obtained on postnatal Day 270. To assess the expression of the genes and proteins, real-time RT-PCR and Western blot were performed respectively. Moreover, adherens junction proteins were localized by immunohistochemistry. In response to flutamide, CDH1 and CTNNB1 expressions were down-regulated along the epididymis, mostly in PD2 group (p < 0.001, p < 0.01). In the prostate, CDH1 mRNA and protein expressions were significantly down-regulated (p < 0.01), whereas CTNNB1 mRNA was slightly up-regulated in both flutamide-treated groups. CTNNB1 protein level was markedly elevated in both PD2 (p < 0.001) and PD90 (p < 0.01) groups. In the epididymis, the expression of ST5AR2 and CYP19A1 was down- and up-regulated, respectively (p < 0.05), whereas in the prostate evident decrease in CYP19A1 expression (p < 0.001, p < 0.01, p < 0.05) was demonstrated. In both tissues, membranous immunolocalization of CTNNB1 suggests its involvement in cell–cell adhesion. Overall, flutamide administration resulted in suppression of androgen action in the epididymis and prostate leading to deregulation of CDH1 and CTNNB1 gene expressions which is probably caused by the alterations in the expression of ST5AR2 and CYP19A1 in both reproductive organs.pl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorGórowska-Wójtowicz, Ewelina - 150833 pl
dc.contributor.authorZarzycka, Marta - 104151 pl
dc.contributor.authorChojnacka, Katarzyna - 118682 pl
dc.contributor.authorBilińska, Barbara - 127338 pl
dc.contributor.authorHejmej, Anna - 141886 pl
dc.date.accessioned2015-04-29T10:23:17Z
dc.date.available2015-04-29T10:23:17Z
dc.date.issued2014pl
dc.date.openaccess12
dc.description.accesstimepo opublikowaniu
dc.description.additionalEwelina Górowska-Wójtowicz podpisana nazwiskiem Górowskapl
dc.description.number2pl
dc.description.physical186-197pl
dc.description.publication1,78pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume2pl
dc.identifier.doi10.1111/j.2047-2927.2013.00172.xpl
dc.identifier.eissn2047-2927pl
dc.identifier.issn2047-2919pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/6104
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceOTHER
dc.rights.uri*
dc.share.typeotwarte czasopismo
dc.subject.enprostatepl
dc.subject.entestosteronepl
dc.subject.enpig epididymispl
dc.subject.enflutamidepl
dc.subject.enadherens junction proteinspl
dc.subject.enboarpl
dc.subject.enepididymispl
dc.subject.enmetabolism of testosteronepl
dc.subtypeArticlepl
dc.titlePostnatal exposure to flutamide affects CDH1 and CTNNB1 gene expression in adult pig epididymis and prostate and alters metabolism of testosteronepl
dc.title.journalAndrologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T22:41:24Z
dc.abstract.otherpl
In both epididymis and prostate the dynamic cross-talk between the cells is hormonally regulated and, in part, through direct cell-to-cell interactions. Functionality of the male reproductive organs may be affected by exposure to specific chemicals, so-called ‘reprotoxicants’. In this study we tested whether early postnatal and prepubertal exposure to anti-androgen flutamide altered the expression of adherens junction genes encoding E-cadherin (CDH1) and β-catenin (CTNNB1) in adult pig epididymis and prostate. In addition, the expression of mRNAs and proteins for 5α-reductase (ST5AR2) and aromatase (CYP19A1) were examined to show whether flutamide alters metabolism of testosterone. Thus, flutamide was injected into male piglets between Days 2 and 10 and between Days 90 and 98 postnatally (PD2 and PD90; 50 mg/kg bw), tissues that were obtained on postnatal Day 270. To assess the expression of the genes and proteins, real-time RT-PCR and Western blot were performed respectively. Moreover, adherens junction proteins were localized by immunohistochemistry. In response to flutamide, CDH1 and CTNNB1 expressions were down-regulated along the epididymis, mostly in PD2 group (p < 0.001, p < 0.01). In the prostate, CDH1 mRNA and protein expressions were significantly down-regulated (p < 0.01), whereas CTNNB1 mRNA was slightly up-regulated in both flutamide-treated groups. CTNNB1 protein level was markedly elevated in both PD2 (p < 0.001) and PD90 (p < 0.01) groups. In the epididymis, the expression of ST5AR2 and CYP19A1 was down- and up-regulated, respectively (p < 0.05), whereas in the prostate evident decrease in CYP19A1 expression (p < 0.001, p < 0.01, p < 0.05) was demonstrated. In both tissues, membranous immunolocalization of CTNNB1 suggests its involvement in cell–cell adhesion. Overall, flutamide administration resulted in suppression of androgen action in the epididymis and prostate leading to deregulation of CDH1 and CTNNB1 gene expressions which is probably caused by the alterations in the expression of ST5AR2 and CYP19A1 in both reproductive organs.
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Górowska-Wójtowicz, Ewelina - 150833
dc.contributor.authorpl
Zarzycka, Marta - 104151
dc.contributor.authorpl
Chojnacka, Katarzyna - 118682
dc.contributor.authorpl
Bilińska, Barbara - 127338
dc.contributor.authorpl
Hejmej, Anna - 141886
dc.date.accessioned
2015-04-29T10:23:17Z
dc.date.available
2015-04-29T10:23:17Z
dc.date.issuedpl
2014
dc.date.openaccess
12
dc.description.accesstime
po opublikowaniu
dc.description.additionalpl
Ewelina Górowska-Wójtowicz podpisana nazwiskiem Górowska
dc.description.numberpl
2
dc.description.physicalpl
186-197
dc.description.publicationpl
1,78
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
2
dc.identifier.doipl
10.1111/j.2047-2927.2013.00172.x
dc.identifier.eissnpl
2047-2927
dc.identifier.issnpl
2047-2919
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/6104
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
OTHER
dc.rights.uri*
dc.share.type
otwarte czasopismo
dc.subject.enpl
prostate
dc.subject.enpl
testosterone
dc.subject.enpl
pig epididymis
dc.subject.enpl
flutamide
dc.subject.enpl
adherens junction proteins
dc.subject.enpl
boar
dc.subject.enpl
epididymis
dc.subject.enpl
metabolism of testosterone
dc.subtypepl
Article
dc.titlepl
Postnatal exposure to flutamide affects CDH1 and CTNNB1 gene expression in adult pig epididymis and prostate and alters metabolism of testosterone
dc.title.journalpl
Andrology
dc.typepl
JournalArticle
dspace.entity.type
Publication

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