Optimization and validation of a procedure for the determination of total chromium in postmortem material by ETAAS

2013
journal article
article
cris.lastimport.wos2024-04-09T22:50:46Z
dc.abstract.enA study was conducted to optimize and validate a procedure for the determination of total chromium in wet digested samples of human blood and body tissues for forensic toxicological purposes. Five experimental setups of electrothermal atomic absorption spectrometry (ETAAS) using a Solaar MQZe (Thermo Electron Co.) were compared, using the following: (i) no modifier, (ii) magnesium nitrate, (iii) palladium nitrate, (iv) magnesium and palladium nitrate mixture and (v) palladium nitrate and ammonium dihydrogen phosphate mixture as chemical modifiers. Furthermore, for the mineralization process in the Ethos 1 microwave system (Milestone), it was found that the temperature program suggested by the producer should be improved, thus, four experimental setups are presented. Finally, the whole procedure was validated, obtaining a linear range of calibration from 0.22 to 12.0 µg/L, limit of detection at 0.07 µg/L, limit of quantification at 0.22 µg/L, precision (as relative standard deviation) up to 6%, and accuracy of 98.4–104.4% for the analysis of certified material (ClinChek Whole Blood Control Level III, SRM Whole Blood Level II and III and SRM 1577c Bovine Liver) and within a range from 84.5 to 103.2% for the recovery of spiked samples. It was ascertained that modified wet digestion, followed by ETAAS with magnesium nitrate as a modifier (5 µg) with 1,400/2,500°C as the pyrolysis and atomization temperatures, respectively, can be used to determine chromium at reference levels in biological materials (blood and internal organs) and levels found in chronic or acute poisonings with chromium compounds.pl
dc.affiliationWydział Chemii : Zakład Chemii Analitycznejpl
dc.contributor.authorLech, Teresapl
dc.contributor.authorDudek-Adamska, Danuta - 126286 pl
dc.date.accessioned2015-06-19T10:10:03Z
dc.date.available2015-06-19T10:10:03Z
dc.date.issued2013pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number2pl
dc.description.physical97-101pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume37pl
dc.identifier.doi10.1093/jat/bks097pl
dc.identifier.eissn1945-2403pl
dc.identifier.issn0146-4760pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/9903
dc.languageengpl
dc.language.containerengpl
dc.rights.licenceOTHER
dc.share.typeinne
dc.subtypeArticlepl
dc.titleOptimization and validation of a procedure for the determination of total chromium in postmortem material by ETAASpl
dc.title.journalJournal of Analytical Toxicologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T22:50:46Z
dc.abstract.enpl
A study was conducted to optimize and validate a procedure for the determination of total chromium in wet digested samples of human blood and body tissues for forensic toxicological purposes. Five experimental setups of electrothermal atomic absorption spectrometry (ETAAS) using a Solaar MQZe (Thermo Electron Co.) were compared, using the following: (i) no modifier, (ii) magnesium nitrate, (iii) palladium nitrate, (iv) magnesium and palladium nitrate mixture and (v) palladium nitrate and ammonium dihydrogen phosphate mixture as chemical modifiers. Furthermore, for the mineralization process in the Ethos 1 microwave system (Milestone), it was found that the temperature program suggested by the producer should be improved, thus, four experimental setups are presented. Finally, the whole procedure was validated, obtaining a linear range of calibration from 0.22 to 12.0 µg/L, limit of detection at 0.07 µg/L, limit of quantification at 0.22 µg/L, precision (as relative standard deviation) up to 6%, and accuracy of 98.4–104.4% for the analysis of certified material (ClinChek Whole Blood Control Level III, SRM Whole Blood Level II and III and SRM 1577c Bovine Liver) and within a range from 84.5 to 103.2% for the recovery of spiked samples. It was ascertained that modified wet digestion, followed by ETAAS with magnesium nitrate as a modifier (5 µg) with 1,400/2,500°C as the pyrolysis and atomization temperatures, respectively, can be used to determine chromium at reference levels in biological materials (blood and internal organs) and levels found in chronic or acute poisonings with chromium compounds.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Analitycznej
dc.contributor.authorpl
Lech, Teresa
dc.contributor.authorpl
Dudek-Adamska, Danuta - 126286
dc.date.accessioned
2015-06-19T10:10:03Z
dc.date.available
2015-06-19T10:10:03Z
dc.date.issuedpl
2013
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
2
dc.description.physicalpl
97-101
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
37
dc.identifier.doipl
10.1093/jat/bks097
dc.identifier.eissnpl
1945-2403
dc.identifier.issnpl
0146-4760
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/9903
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights.licence
OTHER
dc.share.type
inne
dc.subtypepl
Article
dc.titlepl
Optimization and validation of a procedure for the determination of total chromium in postmortem material by ETAAS
dc.title.journalpl
Journal of Analytical Toxicology
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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