Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice

2014
journal article
article
33
cris.lastimport.wos2024-04-10T02:49:45Z
dc.abstract.enLipid rafts (LRs) are dynamic, sterol- and sphingolipid-enriched nanodomains involved in the regulation of cellular functions and signal transduction, that upon stimuli, via (e.g. association of raft proteins and lipids), may cluster into domains of submicron or micron scale. Up to date, however, lipid raft clusters were observed only under artificially promoted conditions and their formation in vivo has not been confirmed. Using non-destructive approach involving Raman and Atomic Force Microscopy imaging we demonstrated the presence of clustered lipid rafts in endothelium of the aorta of the db/db mice that represent a reliable murine model of type 2 diabetes. The raft clusters in the aorta of diabetic mice were shown to occupy a considerably larger (about 10-fold) area of endothelial cells surface as compared to the control. Observation of pathology-promoted LRs confirms that the cellular increase of lipid content results in clustering of LRs. Clustering of LRs leads to the formation of assemblies with diameters up to 3 micrometers and increased lipid character. This massive clustering of lipid rafts in diabetes may trigger a signaling cascade leading to vascular inflammation.pl
dc.affiliationWydział Chemii : Zakład Fizyki Chemicznejpl
dc.affiliationWydział Chemii : Zakład Chemii Fizycznej i Elektrochemiipl
dc.affiliationPion Rektora : Jagiellońskie Centrum Rozwoju Lekówpl
dc.affiliationWydział Lekarski : Zakład Farmakologii Doświadczalnejpl
dc.cm.id68151
dc.contributor.authorPilarczyk, Martapl
dc.contributor.authorMateuszuk, Łukasz - 159343 pl
dc.contributor.authorRyguła, Anna - 161323 pl
dc.contributor.authorKępczyński, Mariusz - 128750 pl
dc.contributor.authorChłopicki, Stefan - 128995 pl
dc.contributor.authorBarańska, Małgorzata - 127224 pl
dc.contributor.authorKaczor, Agnieszka - 128583 pl
dc.date.accession2019-05-14pl
dc.date.accessioned2015-06-12T06:22:53Z
dc.date.available2015-06-12T06:22:53Z
dc.date.issued2014pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.admin[AB] Pilarczyk, Marta 50000141pl
dc.description.number8pl
dc.description.points40pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume9pl
dc.identifier.articleide106065pl
dc.identifier.doi10.1371/journal.pone.0106065pl
dc.identifier.eissn1932-6203pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/9476
dc.identifier.weblinkhttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0106065&type=printablepl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subtypeArticlepl
dc.titleEndothelium in spots : high-content imaging of lipid rafts clusters in db/db micepl
dc.title.journalPLoS ONEpl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T02:49:45Z
dc.abstract.enpl
Lipid rafts (LRs) are dynamic, sterol- and sphingolipid-enriched nanodomains involved in the regulation of cellular functions and signal transduction, that upon stimuli, via (e.g. association of raft proteins and lipids), may cluster into domains of submicron or micron scale. Up to date, however, lipid raft clusters were observed only under artificially promoted conditions and their formation in vivo has not been confirmed. Using non-destructive approach involving Raman and Atomic Force Microscopy imaging we demonstrated the presence of clustered lipid rafts in endothelium of the aorta of the db/db mice that represent a reliable murine model of type 2 diabetes. The raft clusters in the aorta of diabetic mice were shown to occupy a considerably larger (about 10-fold) area of endothelial cells surface as compared to the control. Observation of pathology-promoted LRs confirms that the cellular increase of lipid content results in clustering of LRs. Clustering of LRs leads to the formation of assemblies with diameters up to 3 micrometers and increased lipid character. This massive clustering of lipid rafts in diabetes may trigger a signaling cascade leading to vascular inflammation.
dc.affiliationpl
Wydział Chemii : Zakład Fizyki Chemicznej
dc.affiliationpl
Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii
dc.affiliationpl
Pion Rektora : Jagiellońskie Centrum Rozwoju Leków
dc.affiliationpl
Wydział Lekarski : Zakład Farmakologii Doświadczalnej
dc.cm.id
68151
dc.contributor.authorpl
Pilarczyk, Marta
dc.contributor.authorpl
Mateuszuk, Łukasz - 159343
dc.contributor.authorpl
Ryguła, Anna - 161323
dc.contributor.authorpl
Kępczyński, Mariusz - 128750
dc.contributor.authorpl
Chłopicki, Stefan - 128995
dc.contributor.authorpl
Barańska, Małgorzata - 127224
dc.contributor.authorpl
Kaczor, Agnieszka - 128583
dc.date.accessionpl
2019-05-14
dc.date.accessioned
2015-06-12T06:22:53Z
dc.date.available
2015-06-12T06:22:53Z
dc.date.issuedpl
2014
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.adminpl
[AB] Pilarczyk, Marta 50000141
dc.description.numberpl
8
dc.description.pointspl
40
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
9
dc.identifier.articleidpl
e106065
dc.identifier.doipl
10.1371/journal.pone.0106065
dc.identifier.eissnpl
1932-6203
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/9476
dc.identifier.weblinkpl
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0106065&type=printable
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice
dc.title.journalpl
PLoS ONE
dc.typepl
JournalArticle
dspace.entity.type
Publication

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