Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field

2010
journal article
article
3
cris.lastimport.wos2024-04-10T00:56:07Z
dc.abstract.enThis study describes a technique which makes it possible to introduce the amyloid-like order to protein aggregates by using the scaffolding framework built from supramolecular, fibrillar Congo red structures arranged in an electric field. The electric field was used not only to obtain a uniform orientation of the charged dye fibrils, but also to make the fibrils long, compact and rigid due to the delocalization of pi electrons, which favors ring stacking and, as a consequence, results in an increased tendency to self-assemble. The protein molecules (immunoglobulin L chain lambda, ferritin) attached to this easily adsorbing dye framework assume its ordered structure. The complex precipitating as plate-like fragments shows birefringence in polarized light. The parallel organization of fibrils can be observed with an electron microscope. The dye framework may be removed via reduction with sodium dithionite, leaving the aggregated protein molecules in the ordered state, as confirmed by X-ray diffraction studies.pl
dc.affiliationWydział Chemii : Zakład Krystalochemii i Krystalofizykipl
dc.affiliationWydział Lekarski : Zakład Bioinformatyki i Telemedycynypl
dc.affiliationWydział Lekarski : Katedra Biochemii Lekarskiejpl
dc.affiliationWydział Lekarski : Zakład Chemii Fizjologicznejpl
dc.contributor.authorStopa, Barbara - 133516 pl
dc.contributor.authorPiekarska, Barbara - 133138 pl
dc.contributor.authorKonieczny, Leszekpl
dc.contributor.authorKról, Marcinpl
dc.contributor.authorRybarska, Janinapl
dc.contributor.authorJagusiak, Anna - 129789 pl
dc.contributor.authorSpólnik, Paweł - 133465 pl
dc.contributor.authorRoterman-Konieczna, Irena - 133298 pl
dc.contributor.authorUrbanowicz, Barbarapl
dc.contributor.authorPiwowar, Piotrpl
dc.contributor.authorLewiński, Krzysztof - 129948 pl
dc.date.accession2019-04-26pl
dc.date.accessioned2019-04-08T11:27:40Z
dc.date.available2019-04-08T11:27:40Z
dc.date.issued2010pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. s. 48-50. Na publikacji autorka Roterman-Konieczna Irena podpisana jako Roterman Irena.pl
dc.description.number1pl
dc.description.physical41-50pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume8pl
dc.identifier.doi10.2478/s11532-009-0107-ypl
dc.identifier.eissn1644-3624pl
dc.identifier.issn1895-1066pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/72518
dc.identifier.weblinkhttps://www.degruyter.com/downloadpdf/j/chem.2010.8.issue-1/s11532-009-0107-y/s11532-009-0107-y.pdfpl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska*
dc.rights.licenceCC-BY-NC-ND
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/legalcode*
dc.share.typeotwarte czasopismo
dc.subject.enCongo redpl
dc.subject.enamyloid formationpl
dc.subject.enelectric fieldpl
dc.subtypeArticlepl
dc.titleFormation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric fieldpl
dc.title.journalCentral European Journal of Chemistrypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T00:56:07Z
dc.abstract.enpl
This study describes a technique which makes it possible to introduce the amyloid-like order to protein aggregates by using the scaffolding framework built from supramolecular, fibrillar Congo red structures arranged in an electric field. The electric field was used not only to obtain a uniform orientation of the charged dye fibrils, but also to make the fibrils long, compact and rigid due to the delocalization of pi electrons, which favors ring stacking and, as a consequence, results in an increased tendency to self-assemble. The protein molecules (immunoglobulin L chain lambda, ferritin) attached to this easily adsorbing dye framework assume its ordered structure. The complex precipitating as plate-like fragments shows birefringence in polarized light. The parallel organization of fibrils can be observed with an electron microscope. The dye framework may be removed via reduction with sodium dithionite, leaving the aggregated protein molecules in the ordered state, as confirmed by X-ray diffraction studies.
dc.affiliationpl
Wydział Chemii : Zakład Krystalochemii i Krystalofizyki
dc.affiliationpl
Wydział Lekarski : Zakład Bioinformatyki i Telemedycyny
dc.affiliationpl
Wydział Lekarski : Katedra Biochemii Lekarskiej
dc.affiliationpl
Wydział Lekarski : Zakład Chemii Fizjologicznej
dc.contributor.authorpl
Stopa, Barbara - 133516
dc.contributor.authorpl
Piekarska, Barbara - 133138
dc.contributor.authorpl
Konieczny, Leszek
dc.contributor.authorpl
Król, Marcin
dc.contributor.authorpl
Rybarska, Janina
dc.contributor.authorpl
Jagusiak, Anna - 129789
dc.contributor.authorpl
Spólnik, Paweł - 133465
dc.contributor.authorpl
Roterman-Konieczna, Irena - 133298
dc.contributor.authorpl
Urbanowicz, Barbara
dc.contributor.authorpl
Piwowar, Piotr
dc.contributor.authorpl
Lewiński, Krzysztof - 129948
dc.date.accessionpl
2019-04-26
dc.date.accessioned
2019-04-08T11:27:40Z
dc.date.available
2019-04-08T11:27:40Z
dc.date.issuedpl
2010
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. s. 48-50. Na publikacji autorka Roterman-Konieczna Irena podpisana jako Roterman Irena.
dc.description.numberpl
1
dc.description.physicalpl
41-50
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
8
dc.identifier.doipl
10.2478/s11532-009-0107-y
dc.identifier.eissnpl
1644-3624
dc.identifier.issnpl
1895-1066
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/72518
dc.identifier.weblinkpl
https://www.degruyter.com/downloadpdf/j/chem.2010.8.issue-1/s11532-009-0107-y/s11532-009-0107-y.pdf
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska
dc.rights.licence
CC-BY-NC-ND
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode
dc.share.type
otwarte czasopismo
dc.subject.enpl
Congo red
dc.subject.enpl
amyloid formation
dc.subject.enpl
electric field
dc.subtypepl
Article
dc.titlepl
Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field
dc.title.journalpl
Central European Journal of Chemistry
dc.typepl
JournalArticle
dspace.entity.type
Publication

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