Bradykinin and enhance the adhesion of polymorphonuclear leukocytes to extracellular matrix proteins and endothelial cells

2011
journal article
article
11
dc.abstract.enBradykinin-related peptides (kinins) are well known to contribute to leukocyte recruitment to inflammatory foci; however, a role of these universal pro-inflammatory mediators in the first step of this process, i.e. the leukocyte adhesion to endothelial cells, is not well understood. In this work we found that bradykinin and $des-Arg^{10}-kallidin$ enhance the adhesion of polymorphonuclear bloods cells (PMN) to fibrinogen and fibronectin. Also, the PMN adherence to endothelial cells of HMEC-1 line strongly increased after stimulation by kinins, particularly $des-Arg^{10}-kallidinn$, or when PMN were co-stimulated with bradykinin and $interleukin-1\beta$. These effects were attenuated after PMN treatment with a specific inhibitor of carboxypeptidases, which convert kinins to their des-Arg metabolites. The kinin peptides were also able to change the Mac-1 integrin expression on the PMN surface. These results suggest a regulatory effect of kinins on leukocyte adhesion to endothelial wall, providing new aspects of the leukocyte infiltration into inflamed tissues.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologiipl
dc.contributor.authorGuevara Lora, Ibeth - 128236 pl
dc.contributor.authorŁabędź-Masłowska, Anna - 108413 pl
dc.contributor.authorSkrzeczyńska-Moncznik, Joanna - 131914 pl
dc.contributor.authorKozik, Andrzej - 129351 pl
dc.date.accessioned2018-10-26T05:17:59Z
dc.date.available2018-10-26T05:17:59Z
dc.date.issued2011pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. s. 70-71pl
dc.description.number4pl
dc.description.physical67-71pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume18pl
dc.identifier.doi10.3109/15419061.2011.617854pl
dc.identifier.eissn1543-5180pl
dc.identifier.issn1541-9061pl
dc.identifier.projectROD UJ / Opl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/58739
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceOTHER
dc.rights.uri*
dc.share.typeinne
dc.subject.encell adhesionpl
dc.subject.encytokinepl
dc.subject.enkininspl
dc.subject.enpolymorphonuclear leukocytes inflammationpl
dc.subtypeArticlepl
dc.titleBradykinin and $des-Arg^{10}-kallidin$ enhance the adhesion of polymorphonuclear leukocytes to extracellular matrix proteins and endothelial cellspl
dc.title.journalCell Communication & Adhesionpl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Bradykinin-related peptides (kinins) are well known to contribute to leukocyte recruitment to inflammatory foci; however, a role of these universal pro-inflammatory mediators in the first step of this process, i.e. the leukocyte adhesion to endothelial cells, is not well understood. In this work we found that bradykinin and $des-Arg^{10}-kallidin$ enhance the adhesion of polymorphonuclear bloods cells (PMN) to fibrinogen and fibronectin. Also, the PMN adherence to endothelial cells of HMEC-1 line strongly increased after stimulation by kinins, particularly $des-Arg^{10}-kallidinn$, or when PMN were co-stimulated with bradykinin and $interleukin-1\beta$. These effects were attenuated after PMN treatment with a specific inhibitor of carboxypeptidases, which convert kinins to their des-Arg metabolites. The kinin peptides were also able to change the Mac-1 integrin expression on the PMN surface. These results suggest a regulatory effect of kinins on leukocyte adhesion to endothelial wall, providing new aspects of the leukocyte infiltration into inflamed tissues.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii
dc.contributor.authorpl
Guevara Lora, Ibeth - 128236
dc.contributor.authorpl
Łabędź-Masłowska, Anna - 108413
dc.contributor.authorpl
Skrzeczyńska-Moncznik, Joanna - 131914
dc.contributor.authorpl
Kozik, Andrzej - 129351
dc.date.accessioned
2018-10-26T05:17:59Z
dc.date.available
2018-10-26T05:17:59Z
dc.date.issuedpl
2011
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. s. 70-71
dc.description.numberpl
4
dc.description.physicalpl
67-71
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
18
dc.identifier.doipl
10.3109/15419061.2011.617854
dc.identifier.eissnpl
1543-5180
dc.identifier.issnpl
1541-9061
dc.identifier.projectpl
ROD UJ / O
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/58739
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
OTHER
dc.rights.uri*
dc.share.type
inne
dc.subject.enpl
cell adhesion
dc.subject.enpl
cytokine
dc.subject.enpl
kinins
dc.subject.enpl
polymorphonuclear leukocytes inflammation
dc.subtypepl
Article
dc.titlepl
Bradykinin and $des-Arg^{10}-kallidin$ enhance the adhesion of polymorphonuclear leukocytes to extracellular matrix proteins and endothelial cells
dc.title.journalpl
Cell Communication & Adhesion
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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