Training-induced acceleration of O_{2} uptake on-kinetics precedes muscle mitochondrial biogenesis in humans

2013
journal article
article
49
dc.abstract.enWhat is the central question of this study? A few weeks of endurance training accelerate the oxygen uptake (V_{o_{2}}) on-kinetics in humans. The main aim of the present study was to determine whether the acceleration of V_{o_{2}} on-kinetics obtained by a short period of moderate-intensity training can be explained by an intensification of mitochondrial biogenesis. What is the main finding and its importance? We demonstrated that 5 weeks of moderate-intensity training accelerates the V_{o_{2}} on-kinetics during moderate-intensity cycling in the absence of enhanced mitochondrial biogenesis or capillarization in the trained muscles. We postulate that in the early stages of training an intensification of parallel activation' of oxidative phosphorylation could account for the shortening of the V_{o_{2}} on-transient. The effects of 5 weeks of moderate-intensity endurance training on pulmonary oxygen uptake kinetics (V_{o_{2}} on-kinetics) were studied in 15 healthy men (mean +/- SD: age 22.7 +/- 1.8 years, body weight 76.4 +/- 8.9 kg and maximal 46.0 +/- 3.7 ml kg^{-1} min^{-1}). Training caused a significant acceleration (P= 0.003) of V_{o_{2}} on-kinetics during moderate-intensity cycling (time constant of the primary' component 30.0 +/- 6.6 versus 22.8 +/- 5.6 s before and after training, respectively) and a significant decrease (P= 0.04) in the amplitude of the primary component (837 +/- 351 versus 801 +/- 330 ml min^{-1}). No changes in myosin heavy chain distribution, muscle fibre capillarization, level of peroxisome proliferator-activated receptor coactivator 1 and other markers of mitochondrial biogenesis (mitochondrial DNA copy number, cytochrome c and cytochrome oxidase subunit I contents) in the vastus lateralis were found after training. A significant downregulation in the content of the sarcoplasmic reticulum ATPase 2 (SERCA2; P= 0.03) and a tendency towards a decrease in SERCA1 (P= 0.055) was found after training. The decrease in SERCA1 was positively correlated (P= 0.05) with the training-induced decrease in the gain of the V_{o_{2}} on-kinetics ( \Delta V_{o_{2}} at steady state/\Delta power output). In the early stage of training, the acceleration in V_{o_{2}} on-kinetics during moderate-intensity cycling can occur without enhanced mitochondrial biogenesis or changes in muscle myosin heavy chain distribution and in muscle fibre capillarization. The training-induced decrease of the O_{2} cost of cycling could be caused by the downregulation of SERCA pumps.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki Molekularnejpl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorŻołądź, Jerzy A.pl
dc.contributor.authorGrassi, Brunopl
dc.contributor.authorMajerczak, Joannapl
dc.contributor.authorSzkutnik, Zbigniewpl
dc.contributor.authorKorostyński, Michałpl
dc.contributor.authorKarasiński, Janusz - 128646 pl
dc.contributor.authorKilarski, Wincenty - 152887 pl
dc.contributor.authorKorzeniewski, Bernard - 129166 pl
dc.date.accessioned2015-04-09T11:49:32Z
dc.date.available2015-04-09T11:49:32Z
dc.date.issued2013pl
dc.date.openaccess12
dc.description.accesstimepo opublikowaniu
dc.description.number4pl
dc.description.physical883-898pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume98pl
dc.identifier.doi10.1113/expphysiol.2012.069443pl
dc.identifier.eissn1469-445Xpl
dc.identifier.issn0958-0670pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/4723
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceOTHER
dc.rights.uri*
dc.share.typeotwarte czasopismo
dc.subject.enhuman skeletal musclepl
dc.subject.enoxygen uptakepl
dc.subject.enmetabolic adaptationspl
dc.subtypeArticlepl
dc.titleTraining-induced acceleration of O_{2} uptake on-kinetics precedes muscle mitochondrial biogenesis in humanspl
dc.title.journalExperimental Physiologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
What is the central question of this study? A few weeks of endurance training accelerate the oxygen uptake (V_{o_{2}}) on-kinetics in humans. The main aim of the present study was to determine whether the acceleration of V_{o_{2}} on-kinetics obtained by a short period of moderate-intensity training can be explained by an intensification of mitochondrial biogenesis. What is the main finding and its importance? We demonstrated that 5 weeks of moderate-intensity training accelerates the V_{o_{2}} on-kinetics during moderate-intensity cycling in the absence of enhanced mitochondrial biogenesis or capillarization in the trained muscles. We postulate that in the early stages of training an intensification of parallel activation' of oxidative phosphorylation could account for the shortening of the V_{o_{2}} on-transient. The effects of 5 weeks of moderate-intensity endurance training on pulmonary oxygen uptake kinetics (V_{o_{2}} on-kinetics) were studied in 15 healthy men (mean +/- SD: age 22.7 +/- 1.8 years, body weight 76.4 +/- 8.9 kg and maximal 46.0 +/- 3.7 ml kg^{-1} min^{-1}). Training caused a significant acceleration (P= 0.003) of V_{o_{2}} on-kinetics during moderate-intensity cycling (time constant of the primary' component 30.0 +/- 6.6 versus 22.8 +/- 5.6 s before and after training, respectively) and a significant decrease (P= 0.04) in the amplitude of the primary component (837 +/- 351 versus 801 +/- 330 ml min^{-1}). No changes in myosin heavy chain distribution, muscle fibre capillarization, level of peroxisome proliferator-activated receptor coactivator 1 and other markers of mitochondrial biogenesis (mitochondrial DNA copy number, cytochrome c and cytochrome oxidase subunit I contents) in the vastus lateralis were found after training. A significant downregulation in the content of the sarcoplasmic reticulum ATPase 2 (SERCA2; P= 0.03) and a tendency towards a decrease in SERCA1 (P= 0.055) was found after training. The decrease in SERCA1 was positively correlated (P= 0.05) with the training-induced decrease in the gain of the V_{o_{2}} on-kinetics ( \Delta V_{o_{2}} at steady state/\Delta power output). In the early stage of training, the acceleration in V_{o_{2}} on-kinetics during moderate-intensity cycling can occur without enhanced mitochondrial biogenesis or changes in muscle myosin heavy chain distribution and in muscle fibre capillarization. The training-induced decrease of the O_{2} cost of cycling could be caused by the downregulation of SERCA pumps.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki Molekularnej
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Żołądź, Jerzy A.
dc.contributor.authorpl
Grassi, Bruno
dc.contributor.authorpl
Majerczak, Joanna
dc.contributor.authorpl
Szkutnik, Zbigniew
dc.contributor.authorpl
Korostyński, Michał
dc.contributor.authorpl
Karasiński, Janusz - 128646
dc.contributor.authorpl
Kilarski, Wincenty - 152887
dc.contributor.authorpl
Korzeniewski, Bernard - 129166
dc.date.accessioned
2015-04-09T11:49:32Z
dc.date.available
2015-04-09T11:49:32Z
dc.date.issuedpl
2013
dc.date.openaccess
12
dc.description.accesstime
po opublikowaniu
dc.description.numberpl
4
dc.description.physicalpl
883-898
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
98
dc.identifier.doipl
10.1113/expphysiol.2012.069443
dc.identifier.eissnpl
1469-445X
dc.identifier.issnpl
0958-0670
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/4723
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
OTHER
dc.rights.uri*
dc.share.type
otwarte czasopismo
dc.subject.enpl
human skeletal muscle
dc.subject.enpl
oxygen uptake
dc.subject.enpl
metabolic adaptations
dc.subtypepl
Article
dc.titlepl
Training-induced acceleration of O_{2} uptake on-kinetics precedes muscle mitochondrial biogenesis in humans
dc.title.journalpl
Experimental Physiology
dc.typepl
JournalArticle
dspace.entity.type
Publication

* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.

Views
19
Views per month
Views per city
Ashburn
4
Hong Kong
4
Wroclaw
2
Dublin
1

No access

No Thumbnail Available