Human fibrinogen adsorption on positively charged latex particles

2014
journal article
article
30
30
cris.lastimport.scopus2024-04-07T16:07:50Z
cris.lastimport.wos2024-04-10T01:40:25Z
dc.abstract.otherFibrinogen (Fb) adsorption on positively charged latex particles (average diameter of 800 nm) was studied using the microelectrophoretic and the concentration depletion methods based on AFM imaging. Monolayers on latex were adsorbed from diluted bulk solutions at pH 7.4 and an ionic strength in the range of 10^{−3} to 0.15 M where fibrinogen molecules exhibited an average negative charge. The electrophoretic mobility of the latex after controlled fibrinogen adsorption was systematically measured. A monotonic decrease in the electrophoretic mobility of fibrinogen-covered latex was observed for all ionic strengths. The results of these experiments were interpreted according to the three-dimensional electrokinetic model. It was also determined using the concentration depletion method that fibrinogen adsorption was irreversible and the maximum coverage was equal to 0.6 mg m^{−2} for ionic strength 10^{−3} M and 1.3 mg m^{−2} for ionic strength 0.15 M. The increase of the maximum coverage was confirmed by theoretical modeling based on the random sequential adsorption approach. Paradoxically, the maximum coverage of fibrinogen on positively charged latex particles was more than two times lower than the maximum coverage obtained for negative latex particles (3.2 mg m^{−2}) at pH 7.4 and ionic strength of 0.15 M. This was interpreted as a result of the side-on adsorption of fibrinogen molecules with their negatively charged core attached to the positively charged latex surface. The stability and acid base properties of fibrinogen monolayers on latex were also determined in pH cycling experiments where it was observed that there were no irreversible conformational changes in the fibrinogen monolayers. Additionally, the zeta potential of monolayers was more positive than the zeta potential of fibrinogen in the bulk, which proves a heterogeneous charge distribution. These experimental data reveal a new, side-on adsorption mechanism of fibrinogen on positively charged surfaces and confirmed the decisive role of electrostatic interactions in this process.pl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.contributor.authorŻeliszewska, Paulinapl
dc.contributor.authorBratek-Skicki, Annapl
dc.contributor.authorAdamczyk, Zbigniewpl
dc.contributor.authorCieśla, Michał - 101020 pl
dc.date.accessioned2015-03-11T08:01:43Z
dc.date.available2015-03-11T08:01:43Z
dc.date.issued2014pl
dc.description.physical11165-11174pl
dc.description.volume30 (37)pl
dc.identifier.doi10.1021/la5025668pl
dc.identifier.eissn1520-5827pl
dc.identifier.issn0743-7463pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/3682
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subject.enadsorptionpl
dc.subject.enfibrinogenpl
dc.subject.enRSApl
dc.subtypeArticlepl
dc.titleHuman fibrinogen adsorption on positively charged latex particlespl
dc.title.journalLangmuirpl
dc.typeJournalArticlepl
dspace.entity.typePublication
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