Towards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayers

2012
journal article
article
11
dc.abstract.enThree single-chained ether lipids of comparable chemical structure but different biological activities (namely natural platelet activating factor - PAF, its deacetylated precursor - lyso-PAF and synthetic compound - edelfosine - ED) have been investigated in mixed Langmuir monolayers with phosphatidylethanolamines, PEs (DSPE, SOPE and DOPE), serving as model of inner shell of cellular membrane. The aim of undertaken studies was to verify the correlation between minor differences in chemical structures of the investigated ether lipids and their behavior in membrane-mimicking environment. To reach this goal the interactions between particular ether lipids and PEs have been analyzed with $\Delta G^{Exc}$ values derived from the surface pressure-area isotherms. To get insight into miscibility between film components, Brewster angle microscopy, enabling direct visualization of monolayers structure, has been applied. The obtained results prove significant differences in both mixing properties and the interactions in the investigated systems. On one hand,they are related to the structure of polar head-groups of the studied ether lipids, which determine their capability of hydrogen bond(s) formation with head-groups of PEs. Edelfosine, lacking this property, interacts with PEs the most unfavorably among all the investigated compounds. Another important parameter in this context is the structure of PEs monolayers - the most closely packed DSPE film was found to be most unfavorable for incorporation of ether lipid molecules. Our results prove that the analysis of the interaction between ether lipids and components of biomembrane in Langmuir monolayers is a potent method to explain differences in biological activity of the investigated ether lipids.pl
dc.affiliationWydział Chemii : Zakład Chemii Ogólnejpl
dc.affiliationWydział Chemii : Zakład Chemii Fizycznej i Elektrochemiipl
dc.affiliationWydział Chemii : Zakład Chemii Środowiskapl
dc.contributor.authorHąc-Wydro, Katarzyna - 128297 pl
dc.contributor.authorFlasiński, Michał - 170965 pl
dc.contributor.authorWydro, Paweł - 132797 pl
dc.contributor.authorDynarowicz-Łątka, Patrycja - 127845 pl
dc.date.accessioned2015-02-26T09:10:05Z
dc.date.available2015-02-26T09:10:05Z
dc.date.issued2012pl
dc.description.physical162-170pl
dc.description.volume97pl
dc.identifier.doi10.1016/j.colsurfb.2012.04.023pl
dc.identifier.eissn1873-4367pl
dc.identifier.issn0927-7765pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/3343
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subject.ensingle-chained ether phospholipidspl
dc.subject.enphosphatidylethanolaminespl
dc.subject.enLangmuir monolayerspl
dc.subject.enBrewster angle microscopypl
dc.subtypeArticlepl
dc.titleTowards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayerspl
dc.title.journalColloids and Surfaces. B, Biointerfacespl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Three single-chained ether lipids of comparable chemical structure but different biological activities (namely natural platelet activating factor - PAF, its deacetylated precursor - lyso-PAF and synthetic compound - edelfosine - ED) have been investigated in mixed Langmuir monolayers with phosphatidylethanolamines, PEs (DSPE, SOPE and DOPE), serving as model of inner shell of cellular membrane. The aim of undertaken studies was to verify the correlation between minor differences in chemical structures of the investigated ether lipids and their behavior in membrane-mimicking environment. To reach this goal the interactions between particular ether lipids and PEs have been analyzed with $\Delta G^{Exc}$ values derived from the surface pressure-area isotherms. To get insight into miscibility between film components, Brewster angle microscopy, enabling direct visualization of monolayers structure, has been applied. The obtained results prove significant differences in both mixing properties and the interactions in the investigated systems. On one hand,they are related to the structure of polar head-groups of the studied ether lipids, which determine their capability of hydrogen bond(s) formation with head-groups of PEs. Edelfosine, lacking this property, interacts with PEs the most unfavorably among all the investigated compounds. Another important parameter in this context is the structure of PEs monolayers - the most closely packed DSPE film was found to be most unfavorable for incorporation of ether lipid molecules. Our results prove that the analysis of the interaction between ether lipids and components of biomembrane in Langmuir monolayers is a potent method to explain differences in biological activity of the investigated ether lipids.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Ogólnej
dc.affiliationpl
Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii
dc.affiliationpl
Wydział Chemii : Zakład Chemii Środowiska
dc.contributor.authorpl
Hąc-Wydro, Katarzyna - 128297
dc.contributor.authorpl
Flasiński, Michał - 170965
dc.contributor.authorpl
Wydro, Paweł - 132797
dc.contributor.authorpl
Dynarowicz-Łątka, Patrycja - 127845
dc.date.accessioned
2015-02-26T09:10:05Z
dc.date.available
2015-02-26T09:10:05Z
dc.date.issuedpl
2012
dc.description.physicalpl
162-170
dc.description.volumepl
97
dc.identifier.doipl
10.1016/j.colsurfb.2012.04.023
dc.identifier.eissnpl
1873-4367
dc.identifier.issnpl
0927-7765
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/3343
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
bez licencji
dc.rights.uri*
dc.subject.enpl
single-chained ether phospholipids
dc.subject.enpl
phosphatidylethanolamines
dc.subject.enpl
Langmuir monolayers
dc.subject.enpl
Brewster angle microscopy
dc.subtypepl
Article
dc.titlepl
Towards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayers
dc.title.journalpl
Colloids and Surfaces. B, Biointerfaces
dc.typepl
JournalArticle
dspace.entity.type
Publication

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