Externalization of phosphatidylserine from inner to outer layer may alter the effect of plant sterols on human erythrocyte membrane : the Langmuir monolayer studies

2012
journal article
article
9
dc.abstract.enOne of the factors, which can strongly modify the cell membrane composition, is disordering in membrane asymmetry, resulting from redistribution of lipids from inner to outer layer. Such a disturbance may affect the behavior of various biologically active compounds incorporating into membranes. In this contribution, the relationship between the amounts of phosphatidylserine (PS) in the model outer layer of human eryth- rocyte (RBC) membrane and the effect induced by a plant sterol ($\beta$-sitosterol) was verified. The experiments were performed on multicomponent Langmuir films imitating red blood cell (RBC) membrane, differing in the contents of PS (0%; 5% and 10%) into which the plant sterol was incorporated in various concentrations. The analysis of experimental results (surface pressure–area isotherms complemented with Brewster Angle Microscopy (BAM) proved that the presence of phosphatidylserine molecules, depending on their contents in the mixed monolayer mimicking RBC membrane, changes its properties and exerts in fluence on the effect of plant sterol on the model system. The addition of phytosterol into the monolayer that lacks or contains only 5% of PS was found to be of rather weak effect on the properties of the system. However, in the case of the model membrane containing the increased amount (10%) of PS, the incorporation of plant sterol strongly affects the interactions between molecules and caused thermodynamic destabilization of the mono- layer imitating RBC membrane. These results allow one to suggest that externalization of phosphatidyserine to the outer membrane lea flet may differentiate the effect of plant sterols on cell membranes of various originspl
dc.affiliationWydział Chemii : Zakład Chemii Ogólnejpl
dc.affiliationWydział Chemii : Zakład Chemii Środowiskapl
dc.contributor.authorHąc-Wydro, Katarzyna - 128297 pl
dc.contributor.authorDynarowicz-Łątka, Patrycja - 127845 pl
dc.date.accessioned2015-02-20T12:45:19Z
dc.date.available2015-02-20T12:45:19Z
dc.date.issued2012pl
dc.date.openaccess12
dc.description.accesstimepo opublikowaniu
dc.description.number9pl
dc.description.physical2184-2191pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume1818pl
dc.identifier.doi10.1016/j.bbamem.2012.05.010pl
dc.identifier.eissn1879-2642pl
dc.identifier.issn0005-2736pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/3254
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceOTHER
dc.rights.uri*
dc.share.typeotwarte czasopismo
dc.subject.enmodel outer layer of human erythrocytepl
dc.subject.enlangmuir filmpl
dc.subject.en(RBC) membranepl
dc.subject.enBrewster angle microscopypl
dc.subject.enplant sterolpl
dc.subject.enPhosphatidylserinepl
dc.subtypeArticlepl
dc.titleExternalization of phosphatidylserine from inner to outer layer may alter the effect of plant sterols on human erythrocyte membrane : the Langmuir monolayer studiespl
dc.title.journalBiochimica et Biophysica Acta. Biomembranespl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
One of the factors, which can strongly modify the cell membrane composition, is disordering in membrane asymmetry, resulting from redistribution of lipids from inner to outer layer. Such a disturbance may affect the behavior of various biologically active compounds incorporating into membranes. In this contribution, the relationship between the amounts of phosphatidylserine (PS) in the model outer layer of human eryth- rocyte (RBC) membrane and the effect induced by a plant sterol ($\beta$-sitosterol) was verified. The experiments were performed on multicomponent Langmuir films imitating red blood cell (RBC) membrane, differing in the contents of PS (0%; 5% and 10%) into which the plant sterol was incorporated in various concentrations. The analysis of experimental results (surface pressure–area isotherms complemented with Brewster Angle Microscopy (BAM) proved that the presence of phosphatidylserine molecules, depending on their contents in the mixed monolayer mimicking RBC membrane, changes its properties and exerts in fluence on the effect of plant sterol on the model system. The addition of phytosterol into the monolayer that lacks or contains only 5% of PS was found to be of rather weak effect on the properties of the system. However, in the case of the model membrane containing the increased amount (10%) of PS, the incorporation of plant sterol strongly affects the interactions between molecules and caused thermodynamic destabilization of the mono- layer imitating RBC membrane. These results allow one to suggest that externalization of phosphatidyserine to the outer membrane lea flet may differentiate the effect of plant sterols on cell membranes of various origins
dc.affiliationpl
Wydział Chemii : Zakład Chemii Ogólnej
dc.affiliationpl
Wydział Chemii : Zakład Chemii Środowiska
dc.contributor.authorpl
Hąc-Wydro, Katarzyna - 128297
dc.contributor.authorpl
Dynarowicz-Łątka, Patrycja - 127845
dc.date.accessioned
2015-02-20T12:45:19Z
dc.date.available
2015-02-20T12:45:19Z
dc.date.issuedpl
2012
dc.date.openaccess
12
dc.description.accesstime
po opublikowaniu
dc.description.numberpl
9
dc.description.physicalpl
2184-2191
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
1818
dc.identifier.doipl
10.1016/j.bbamem.2012.05.010
dc.identifier.eissnpl
1879-2642
dc.identifier.issnpl
0005-2736
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/3254
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
OTHER
dc.rights.uri*
dc.share.type
otwarte czasopismo
dc.subject.enpl
model outer layer of human erythrocyte
dc.subject.enpl
langmuir film
dc.subject.enpl
(RBC) membrane
dc.subject.enpl
Brewster angle microscopy
dc.subject.enpl
plant sterol
dc.subject.enpl
Phosphatidylserine
dc.subtypepl
Article
dc.titlepl
Externalization of phosphatidylserine from inner to outer layer may alter the effect of plant sterols on human erythrocyte membrane : the Langmuir monolayer studies
dc.title.journalpl
Biochimica et Biophysica Acta. Biomembranes
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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