Red blood cells polarize green laser light revealing hemoglobin's enhanced non-fundamental Raman modes

2014
journal article
article
30
cris.lastimport.wos2024-04-09T20:46:15Z
dc.abstract.enIn general, the first overtone modes produce weak bands that appear at approximately twice the wavenumber value of the fundamental transitions in vibrational spectra. Here, we report the existence of a series of enhanced non-fundamental bands in resonance Raman (RR) spectra recorded for hemoglobin (Hb) inside the highly concentrated heme environment of the red blood cell (RBC) by exciting with a 514.5 nm laser line. Such bands are most intense when detecting parallel-polarized light. The enhancement is explained through excitonic theory invoking a type C scattering mechanism and bands have been assigned to overtone and combination bands based on symmetry arguments and polarization measurements. By using malaria diagnosis as an example, we demonstrate that combining the non-fundamental and fundamental regions of the RR spectrum improves the sensitivity and diagnostic capability of the technique. The discovery will have considerable implications for the ongoing development of Raman spectroscopy for blood disease diagnoses and monitoring heme perturbation in response to environmental stimuli.pl
dc.affiliationWydział Chemii : Zakład Fizyki Chemicznejpl
dc.affiliationPion Rektora : Jagiellońskie Centrum Rozwoju Lekówpl
dc.contributor.authorMarzec, Katarzyna M. - 145326 pl
dc.contributor.authorPerez-Guaita, Davidpl
dc.contributor.authorde Veij, Marleenpl
dc.contributor.authorBarańska, Małgorzata - 127224 pl
dc.contributor.authorDixon, Matthew W. A.pl
dc.contributor.authorTilley, Leannpl
dc.contributor.authorWood, Bayden R.pl
dc.contributor.authorMcNaughton, Donpl
dc.date.accession2023-12-13pl
dc.date.accessioned2015-02-20T10:38:53Z
dc.date.available2015-02-20T10:38:53Z
dc.date.issued2014pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number18pl
dc.description.physical3963-3968pl
dc.description.points35pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume15pl
dc.identifier.doi10.1002/cphc.201402598pl
dc.identifier.eissn1439-7641pl
dc.identifier.issn1439-4235pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/3248
dc.identifier.weblinkhttps://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cphc.201402598pl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne*
dc.rights.licenceCC-BY-NC
dc.rights.urihttps://creativecommons.org/licenses*
dc.share.typeinne
dc.subject.enmalaria diagnosticpl
dc.subject.enovertone/combination modespl
dc.subject.enporphyrinoidspl
dc.subject.enRaman spectroscopypl
dc.subject.enred blood cellspl
dc.subtypeArticlepl
dc.titleRed blood cells polarize green laser light revealing hemoglobin's enhanced non-fundamental Raman modespl
dc.title.journalChemPhysChempl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T20:46:15Z
dc.abstract.enpl
In general, the first overtone modes produce weak bands that appear at approximately twice the wavenumber value of the fundamental transitions in vibrational spectra. Here, we report the existence of a series of enhanced non-fundamental bands in resonance Raman (RR) spectra recorded for hemoglobin (Hb) inside the highly concentrated heme environment of the red blood cell (RBC) by exciting with a 514.5 nm laser line. Such bands are most intense when detecting parallel-polarized light. The enhancement is explained through excitonic theory invoking a type C scattering mechanism and bands have been assigned to overtone and combination bands based on symmetry arguments and polarization measurements. By using malaria diagnosis as an example, we demonstrate that combining the non-fundamental and fundamental regions of the RR spectrum improves the sensitivity and diagnostic capability of the technique. The discovery will have considerable implications for the ongoing development of Raman spectroscopy for blood disease diagnoses and monitoring heme perturbation in response to environmental stimuli.
dc.affiliationpl
Wydział Chemii : Zakład Fizyki Chemicznej
dc.affiliationpl
Pion Rektora : Jagiellońskie Centrum Rozwoju Leków
dc.contributor.authorpl
Marzec, Katarzyna M. - 145326
dc.contributor.authorpl
Perez-Guaita, David
dc.contributor.authorpl
de Veij, Marleen
dc.contributor.authorpl
Barańska, Małgorzata - 127224
dc.contributor.authorpl
Dixon, Matthew W. A.
dc.contributor.authorpl
Tilley, Leann
dc.contributor.authorpl
Wood, Bayden R.
dc.contributor.authorpl
McNaughton, Don
dc.date.accessionpl
2023-12-13
dc.date.accessioned
2015-02-20T10:38:53Z
dc.date.available
2015-02-20T10:38:53Z
dc.date.issuedpl
2014
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
18
dc.description.physicalpl
3963-3968
dc.description.pointspl
35
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
15
dc.identifier.doipl
10.1002/cphc.201402598
dc.identifier.eissnpl
1439-7641
dc.identifier.issnpl
1439-4235
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/3248
dc.identifier.weblinkpl
https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cphc.201402598
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne
dc.rights.licence
CC-BY-NC
dc.rights.uri*
https://creativecommons.org/licenses
dc.share.type
inne
dc.subject.enpl
malaria diagnostic
dc.subject.enpl
overtone/combination modes
dc.subject.enpl
porphyrinoids
dc.subject.enpl
Raman spectroscopy
dc.subject.enpl
red blood cells
dc.subtypepl
Article
dc.titlepl
Red blood cells polarize green laser light revealing hemoglobin's enhanced non-fundamental Raman modes
dc.title.journalpl
ChemPhysChem
dc.typepl
JournalArticle
dspace.entity.type
Publication

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