Preparation of tissue samples for X-ray fluorescence microscopy

2005
journal article
article
64
dc.abstract.enAs is well-known, trace elements, especially metals, play an important role in the pathogenesis of many disorders. The topographic and quantitative elemental analysis of pathologically changed tissues may shed some new light on processes leading to the degeneration of cells in the case of selected diseases. An ideal and powerful tool for such purpose is the Synchrotron Microbeam X-ray Fluorescence technique. It enables the carrying out of investigations of the elemental composition of tissues even at the single cell level. The tissue samples for histopathological investigations are routinely fixed and embedded in paraffin. The authors try to verify the usefulness of such prepared tissue sections for elemental analysis with the use of X-ray fluorescence microscopy. Studies were performed on rat brain samples. Changes in elemental composition caused by fixation in formalin or paraformaldehyde and embedding in paraffin were examined. Measurements were carried out at the bending magnet beamline L of the Hamburger Synchrotronstrahlungslabor HASYLAB in Hamburg. The decrease in mass per unit area of K, Br and the increase in P, S, Fe, Cu and Zn in the tissue were observed as a result of the fixation. For the samples embedded in paraffin, a lower level of most elements was observed. Additionally, for these samples, changes in the composition of some elements were not uniform for different analyzed areas of rat brain.pl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorChwiej, Joannapl
dc.contributor.authorSzczerbowska-Boruchowska, Magdalenapl
dc.contributor.authorLankosz, Marekpl
dc.contributor.authorWójcik, Sławomirpl
dc.contributor.authorFalkenberg, Geraldpl
dc.contributor.authorStęgowski, Zdzisławpl
dc.contributor.authorSetkowicz-Janeczko, Zuzanna - 131836 pl
dc.date.accessioned2016-11-10T13:20:46Z
dc.date.available2016-11-10T13:20:46Z
dc.date.issued2005pl
dc.description.additionalZuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowiczpl
dc.description.number12pl
dc.description.physical1531-1537pl
dc.description.volume60pl
dc.identifier.doi10.1016/j.sab.2005.10.002pl
dc.identifier.eissn1873-3565pl
dc.identifier.issn0584-8547pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/32277
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subtypeArticlepl
dc.titlePreparation of tissue samples for X-ray fluorescence microscopypl
dc.title.journalSpectrochimica Acta. Part B, Atomic Spectroscopypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
As is well-known, trace elements, especially metals, play an important role in the pathogenesis of many disorders. The topographic and quantitative elemental analysis of pathologically changed tissues may shed some new light on processes leading to the degeneration of cells in the case of selected diseases. An ideal and powerful tool for such purpose is the Synchrotron Microbeam X-ray Fluorescence technique. It enables the carrying out of investigations of the elemental composition of tissues even at the single cell level. The tissue samples for histopathological investigations are routinely fixed and embedded in paraffin. The authors try to verify the usefulness of such prepared tissue sections for elemental analysis with the use of X-ray fluorescence microscopy. Studies were performed on rat brain samples. Changes in elemental composition caused by fixation in formalin or paraformaldehyde and embedding in paraffin were examined. Measurements were carried out at the bending magnet beamline L of the Hamburger Synchrotronstrahlungslabor HASYLAB in Hamburg. The decrease in mass per unit area of K, Br and the increase in P, S, Fe, Cu and Zn in the tissue were observed as a result of the fixation. For the samples embedded in paraffin, a lower level of most elements was observed. Additionally, for these samples, changes in the composition of some elements were not uniform for different analyzed areas of rat brain.
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Chwiej, Joanna
dc.contributor.authorpl
Szczerbowska-Boruchowska, Magdalena
dc.contributor.authorpl
Lankosz, Marek
dc.contributor.authorpl
Wójcik, Sławomir
dc.contributor.authorpl
Falkenberg, Gerald
dc.contributor.authorpl
Stęgowski, Zdzisław
dc.contributor.authorpl
Setkowicz-Janeczko, Zuzanna - 131836
dc.date.accessioned
2016-11-10T13:20:46Z
dc.date.available
2016-11-10T13:20:46Z
dc.date.issuedpl
2005
dc.description.additionalpl
Zuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowicz
dc.description.numberpl
12
dc.description.physicalpl
1531-1537
dc.description.volumepl
60
dc.identifier.doipl
10.1016/j.sab.2005.10.002
dc.identifier.eissnpl
1873-3565
dc.identifier.issnpl
0584-8547
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/32277
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
bez licencji
dc.rights.uri*
dc.subtypepl
Article
dc.titlepl
Preparation of tissue samples for X-ray fluorescence microscopy
dc.title.journalpl
Spectrochimica Acta. Part B, Atomic Spectroscopy
dc.typepl
JournalArticle
dspace.entity.type
Publication

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