Tacrolimus (FK506) and cyclosporin A reduce macrophage recruitment to the rat brain injured at perinatal and early postnatal periods

2009
journal article
article
9
dc.abstract.enObjective: Tacrolimus (FK506) and cyclosporin A (CsA), immunosuppressants widely used in post-transplantional therapy, have been reported to protect neurons in the injured brain. This effect can be exerted directly and indirectly via inflammatory cells. Since the data come exclusively from studies on the adult brain, we examined effects of the drugs on the macrophage recruitment in the brain injured at early developmental stages. Methods: Following the brain injury, 1- and 6-day-old Wistar rats (P1s and P6s, respectively) were treated with FK506 or CsA and injected with [3 H] thymidine. Brain sections were processed for BSI-B4 isolectin histochemistry and subjected to autoradiography to visualize proliferating and non-proliferating macrophages. Results: In P1s (n = 33), FK506 evoked a dose-dependent reduction in the number of macrophages. P6s (n = 30) presented greater decreases in macrophage numbers and their proliferative activity than the newborns. CsA application in P1s (n = 27) affected neither recruitment of macrophages to the region of injury nor their proliferation. In CsA-treated P6s (n = 28), reduction of the macrophage population and its proliferative activity was also seen but was much smaller than that following FK506 administration. Discussion: High effectiveness of FK506 in regulation of the inflammatory response and neuroprotection observed in the adult brain can also be considered as a possible indirect determinant of neuronal survival following the brain injury at very early developmental stages.pl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorSetkowicz-Janeczko, Zuzanna - 131836 pl
dc.contributor.authorCaryk, Mariapl
dc.contributor.authorSzafraniec, Milena - 243364 pl
dc.contributor.authorŻmudzińska, Annapl
dc.contributor.authorJaneczko, Krzysztof - 128444 pl
dc.date.accessioned2016-10-28T10:36:02Z
dc.date.available2016-10-28T10:36:02Z
dc.date.issued2009pl
dc.description.additionalZuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowiczpl
dc.description.number10pl
dc.description.physical1060-1067pl
dc.description.volume31pl
dc.identifier.doi10.1179/174313209X383295pl
dc.identifier.eissn1743-1328pl
dc.identifier.issn0161-6412pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/31864
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subtypeArticlepl
dc.titleTacrolimus (FK506) and cyclosporin A reduce macrophage recruitment to the rat brain injured at perinatal and early postnatal periodspl
dc.title.journalNeurological Researchpl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Objective: Tacrolimus (FK506) and cyclosporin A (CsA), immunosuppressants widely used in post-transplantional therapy, have been reported to protect neurons in the injured brain. This effect can be exerted directly and indirectly via inflammatory cells. Since the data come exclusively from studies on the adult brain, we examined effects of the drugs on the macrophage recruitment in the brain injured at early developmental stages. Methods: Following the brain injury, 1- and 6-day-old Wistar rats (P1s and P6s, respectively) were treated with FK506 or CsA and injected with [3 H] thymidine. Brain sections were processed for BSI-B4 isolectin histochemistry and subjected to autoradiography to visualize proliferating and non-proliferating macrophages. Results: In P1s (n = 33), FK506 evoked a dose-dependent reduction in the number of macrophages. P6s (n = 30) presented greater decreases in macrophage numbers and their proliferative activity than the newborns. CsA application in P1s (n = 27) affected neither recruitment of macrophages to the region of injury nor their proliferation. In CsA-treated P6s (n = 28), reduction of the macrophage population and its proliferative activity was also seen but was much smaller than that following FK506 administration. Discussion: High effectiveness of FK506 in regulation of the inflammatory response and neuroprotection observed in the adult brain can also be considered as a possible indirect determinant of neuronal survival following the brain injury at very early developmental stages.
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Setkowicz-Janeczko, Zuzanna - 131836
dc.contributor.authorpl
Caryk, Maria
dc.contributor.authorpl
Szafraniec, Milena - 243364
dc.contributor.authorpl
Żmudzińska, Anna
dc.contributor.authorpl
Janeczko, Krzysztof - 128444
dc.date.accessioned
2016-10-28T10:36:02Z
dc.date.available
2016-10-28T10:36:02Z
dc.date.issuedpl
2009
dc.description.additionalpl
Zuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowicz
dc.description.numberpl
10
dc.description.physicalpl
1060-1067
dc.description.volumepl
31
dc.identifier.doipl
10.1179/174313209X383295
dc.identifier.eissnpl
1743-1328
dc.identifier.issnpl
0161-6412
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/31864
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
bez licencji
dc.rights.uri*
dc.subtypepl
Article
dc.titlepl
Tacrolimus (FK506) and cyclosporin A reduce macrophage recruitment to the rat brain injured at perinatal and early postnatal periods
dc.title.journalpl
Neurological Research
dc.typepl
JournalArticle
dspace.entity.type
Publication

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