Molecular oxygen as a probe molecule in EPR spin labeling studies of membrane structure and dynamics

2022
journal article
review article
cris.lastimport.wos2024-04-09T20:40:37Z
dc.abstract.enMolecular oxygen ($O_{2}$) is the perfect probe molecule for membrane studies carried out using the saturation recovery EPR technique. O2 is a small, paramagnetic, hydrophobic enough molecule that easily partitions into a membrane’s different phases and domains. In membrane studies, the saturation recovery EPR method requires two paramagnetic probes: a lipid-analog nitroxide spin label and an oxygen molecule. The experimentally derived parameters of this method are the spin–lattice relaxation times ($T_{1}s$) of spin labels and rates of bimolecular collisions between $O_{2}$ and the nitroxide fragment. Thanks to the long T1 of lipid spin labels (from 1 to 10 µs), the approach is very sensitive to changes of the local (around the nitroxide fragment) $O_{2}$ diffusion-concentration product. Small variations in the lipid packing affect $O_{2}$ solubility and $O_{2}$ diffusion, which can be detected by the shortening of $T_{1}$ of spin labels. Using $O_{2}$ as a probe molecule and a different lipid spin label inserted into specific phases of the membrane and membrane domains allows data about the lateral arrangement of lipid membranes to be obtained. Moreover, using a lipid spin label with the nitroxide fragment attached to its head group or a hydrocarbon chain at different positions also enables data about molecular dynamics and structure at different membrane depths to be obtained. Thus, the method can be used to investigate not only the lateral organization of the membrane (i.e., the presence of membrane domains and phases), but also the depth-dependent membrane structure and dynamics and, hence, the membrane properties in three dimensions.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki Obliczeniowej i Bioinformatykipl
dc.contributor.authorSubczynski, Witold K.pl
dc.contributor.authorWidomska, Justynapl
dc.contributor.authorRaguz, Marijapl
dc.contributor.authorPasenkiewicz-Gierula, Marta - 131288 pl
dc.date.accessioned2023-04-12T06:53:39Z
dc.date.available2023-04-12T06:53:39Z
dc.date.issued2022pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. s. 312-316pl
dc.description.number3pl
dc.description.physical295-316pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume2pl
dc.identifier.doi10.3390/oxygen2030021pl
dc.identifier.eissn2673-9801pl
dc.identifier.issn2673-9801pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/310240
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enmolecular oxygenpl
dc.subject.enlipid spin labelspl
dc.subject.enEPRpl
dc.subject.enlipid bilayer membranespl
dc.subject.enmembrane fluiditypl
dc.subject.enmembrane domainspl
dc.subject.encholesterolpl
dc.subtypeReviewArticlepl
dc.titleMolecular oxygen as a probe molecule in EPR spin labeling studies of membrane structure and dynamicspl
dc.title.journalOxygenpl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T20:40:37Z
dc.abstract.enpl
Molecular oxygen ($O_{2}$) is the perfect probe molecule for membrane studies carried out using the saturation recovery EPR technique. O2 is a small, paramagnetic, hydrophobic enough molecule that easily partitions into a membrane’s different phases and domains. In membrane studies, the saturation recovery EPR method requires two paramagnetic probes: a lipid-analog nitroxide spin label and an oxygen molecule. The experimentally derived parameters of this method are the spin–lattice relaxation times ($T_{1}s$) of spin labels and rates of bimolecular collisions between $O_{2}$ and the nitroxide fragment. Thanks to the long T1 of lipid spin labels (from 1 to 10 µs), the approach is very sensitive to changes of the local (around the nitroxide fragment) $O_{2}$ diffusion-concentration product. Small variations in the lipid packing affect $O_{2}$ solubility and $O_{2}$ diffusion, which can be detected by the shortening of $T_{1}$ of spin labels. Using $O_{2}$ as a probe molecule and a different lipid spin label inserted into specific phases of the membrane and membrane domains allows data about the lateral arrangement of lipid membranes to be obtained. Moreover, using a lipid spin label with the nitroxide fragment attached to its head group or a hydrocarbon chain at different positions also enables data about molecular dynamics and structure at different membrane depths to be obtained. Thus, the method can be used to investigate not only the lateral organization of the membrane (i.e., the presence of membrane domains and phases), but also the depth-dependent membrane structure and dynamics and, hence, the membrane properties in three dimensions.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki Obliczeniowej i Bioinformatyki
dc.contributor.authorpl
Subczynski, Witold K.
dc.contributor.authorpl
Widomska, Justyna
dc.contributor.authorpl
Raguz, Marija
dc.contributor.authorpl
Pasenkiewicz-Gierula, Marta - 131288
dc.date.accessioned
2023-04-12T06:53:39Z
dc.date.available
2023-04-12T06:53:39Z
dc.date.issuedpl
2022
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. s. 312-316
dc.description.numberpl
3
dc.description.physicalpl
295-316
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
2
dc.identifier.doipl
10.3390/oxygen2030021
dc.identifier.eissnpl
2673-9801
dc.identifier.issnpl
2673-9801
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/310240
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
molecular oxygen
dc.subject.enpl
lipid spin labels
dc.subject.enpl
EPR
dc.subject.enpl
lipid bilayer membranes
dc.subject.enpl
membrane fluidity
dc.subject.enpl
membrane domains
dc.subject.enpl
cholesterol
dc.subtypepl
ReviewArticle
dc.titlepl
Molecular oxygen as a probe molecule in EPR spin labeling studies of membrane structure and dynamics
dc.title.journalpl
Oxygen
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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