The influence of microinjection parameters on cell survival and procedure efficiency

2023
journal article
article
cris.lastimport.wos2024-04-10T02:34:56Z
dc.abstract.enMicroinjection is a method commonly used to deliver various substances into cells. The procedure is performed on a widefield microscope stage using fine glass needle to penetrate the cell membrane. Microinjection can be carried out using a manual or semi-automatic mode. For commercially available equipment currently reported microinjection success rate and cell viability are relatively low (around 50% for both indicators). Here, for the first time, we systematically show how the microinjection effectiveness and cell viability are influenced by needle diameter and chosen microinjection mode. We found that manual mode entailed a higher injection rate, reducing cell viability at the same time. The reduction in needle diameter caused a significant increase in cell survival rate (from 43 to 73% for manual mode and from 58% to 86% for semi-automatic mode) and did not affect significantly the success rate. Our findings will help optimize this method in the context of cell biology research.pl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.affiliationWydział Farmaceutyczny : Zakład Farmacji Społecznejpl
dc.affiliationSzkoła Doktorska Nauk Ścisłych i Przyrodniczychpl
dc.cm.id111690
dc.cm.idOmegaUJCMe6f099bc0cbd44039544ba934d41184f
dc.cm.idOmegaUJCMe6f099bc0cbd44039544ba934d41184fpl
dc.contributor.authorHajduk, Joanna - 421130 pl
dc.contributor.authorSzajna, Konrad - 167877 pl
dc.contributor.authorLisowski, Bartosz - 149778 pl
dc.contributor.authorRajfur, Zenon - 214965 pl
dc.date.accessioned2023-03-27T10:38:28Z
dc.date.available2023-03-27T10:38:28Z
dc.date.issued2023pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.physical102107pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume10pl
dc.identifier.articleid102107pl
dc.identifier.doi10.1016/j.mex.2023.102107pl
dc.identifier.eissn2215-0161pl
dc.identifier.issn2215-0161pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/309499
dc.languageengpl
dc.language.containerengpl
dc.pbn.affiliationDziedzina nauk medycznych i nauk o zdrowiu : nauki farmaceutyczne
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licence
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enmicroinjectionpl
dc.subject.enmanualpl
dc.subject.ensemi-automaticpl
dc.subject.encell culturepl
dc.subject.enfibroblastspl
dc.subtypeArticlepl
dc.titleThe influence of microinjection parameters on cell survival and procedure efficiencypl
dc.title.journalMethodsXpl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T02:34:56Z
dc.abstract.enpl
Microinjection is a method commonly used to deliver various substances into cells. The procedure is performed on a widefield microscope stage using fine glass needle to penetrate the cell membrane. Microinjection can be carried out using a manual or semi-automatic mode. For commercially available equipment currently reported microinjection success rate and cell viability are relatively low (around 50% for both indicators). Here, for the first time, we systematically show how the microinjection effectiveness and cell viability are influenced by needle diameter and chosen microinjection mode. We found that manual mode entailed a higher injection rate, reducing cell viability at the same time. The reduction in needle diameter caused a significant increase in cell survival rate (from 43 to 73% for manual mode and from 58% to 86% for semi-automatic mode) and did not affect significantly the success rate. Our findings will help optimize this method in the context of cell biology research.
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
dc.affiliationpl
Wydział Farmaceutyczny : Zakład Farmacji Społecznej
dc.affiliationpl
Szkoła Doktorska Nauk Ścisłych i Przyrodniczych
dc.cm.id
111690
dc.cm.idOmega
UJCMe6f099bc0cbd44039544ba934d41184f
dc.cm.idOmegapl
UJCMe6f099bc0cbd44039544ba934d41184f
dc.contributor.authorpl
Hajduk, Joanna - 421130
dc.contributor.authorpl
Szajna, Konrad - 167877
dc.contributor.authorpl
Lisowski, Bartosz - 149778
dc.contributor.authorpl
Rajfur, Zenon - 214965
dc.date.accessioned
2023-03-27T10:38:28Z
dc.date.available
2023-03-27T10:38:28Z
dc.date.issuedpl
2023
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.physicalpl
102107
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
10
dc.identifier.articleidpl
102107
dc.identifier.doipl
10.1016/j.mex.2023.102107
dc.identifier.eissnpl
2215-0161
dc.identifier.issnpl
2215-0161
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/309499
dc.languagepl
eng
dc.language.containerpl
eng
dc.pbn.affiliation
Dziedzina nauk medycznych i nauk o zdrowiu : nauki farmaceutyczne
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
microinjection
dc.subject.enpl
manual
dc.subject.enpl
semi-automatic
dc.subject.enpl
cell culture
dc.subject.enpl
fibroblasts
dc.subtypepl
Article
dc.titlepl
The influence of microinjection parameters on cell survival and procedure efficiency
dc.title.journalpl
MethodsX
dc.typepl
JournalArticle
dspace.entity.type
Publication

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