Morphology of reactive microglia in the injured cerebral cortex : fractal analysis and complementary quantitative methods

160
cris.lastimport.wos2024-04-09T20:03:28Z
dc.abstract.otherThe present study focuses on application of quantitative methods measuring differences between particular morphological types of microglial cells as well as between their proliferating and non-proliferating examples. On the basis of subjective classification, microglial cells of three morphological types (ramified, hypertrophied and bushy) were selected from the neocortex of injured rat brain. Thereafter, the morphological complexity of each cell was assessed by calculation its fractal dimension as well as its form factor, convexity, ramification factor and solidity. The fractal dimension seemed a good parameter for detecting small changes in the space-filing capacity of cells, for example, it shows differences between ramified cells from control and injured brains. This measure seemed insensitive to some aspects of cell morphology. To obtain precise quantification of observed changes other morphological parameters had to be applied. Proliferating and non-proliferating microglial cells displayed significant differences in their solidity and ramification factors, but not in fractal dimension and convexity. The results indicated that proliferating microglia were more massive and less-ramified but they did not reduce their spatial complexity.pl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorSołtys, Zbigniew - 147734 pl
dc.contributor.authorZiaja, Marek - 132904 pl
dc.contributor.authorPawliński, Rafałpl
dc.contributor.authorSetkowicz-Janeczko, Zuzanna - 131836 pl
dc.contributor.authorJaneczko, Krzysztof - 128444 pl
dc.date.accessioned2016-09-28T11:01:04Z
dc.date.available2016-09-28T11:01:04Z
dc.date.issued2001pl
dc.description.additionalZuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowiczpl
dc.description.number1pl
dc.description.physical90-97pl
dc.description.volume63pl
dc.identifier.doi10.1002/1097-4547(20010101)63:1<90::AID-JNR11>3.0.CO;2-9pl
dc.identifier.eissn1097-4547pl
dc.identifier.issn0360-4012pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/30925
dc.languageengpl
dc.language.containerengpl
dc.rights.licencebez licencji
dc.subtypeArticlepl
dc.titleMorphology of reactive microglia in the injured cerebral cortex : fractal analysis and complementary quantitative methodspl
dc.title.journalJournal of Neuroscience Researchpl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T20:03:28Z
dc.abstract.otherpl
The present study focuses on application of quantitative methods measuring differences between particular morphological types of microglial cells as well as between their proliferating and non-proliferating examples. On the basis of subjective classification, microglial cells of three morphological types (ramified, hypertrophied and bushy) were selected from the neocortex of injured rat brain. Thereafter, the morphological complexity of each cell was assessed by calculation its fractal dimension as well as its form factor, convexity, ramification factor and solidity. The fractal dimension seemed a good parameter for detecting small changes in the space-filing capacity of cells, for example, it shows differences between ramified cells from control and injured brains. This measure seemed insensitive to some aspects of cell morphology. To obtain precise quantification of observed changes other morphological parameters had to be applied. Proliferating and non-proliferating microglial cells displayed significant differences in their solidity and ramification factors, but not in fractal dimension and convexity. The results indicated that proliferating microglia were more massive and less-ramified but they did not reduce their spatial complexity.
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Sołtys, Zbigniew - 147734
dc.contributor.authorpl
Ziaja, Marek - 132904
dc.contributor.authorpl
Pawliński, Rafał
dc.contributor.authorpl
Setkowicz-Janeczko, Zuzanna - 131836
dc.contributor.authorpl
Janeczko, Krzysztof - 128444
dc.date.accessioned
2016-09-28T11:01:04Z
dc.date.available
2016-09-28T11:01:04Z
dc.date.issuedpl
2001
dc.description.additionalpl
Zuzanna Setkowicz-Janeczko podpisana: Zuzanna Setkowicz
dc.description.numberpl
1
dc.description.physicalpl
90-97
dc.description.volumepl
63
dc.identifier.doipl
10.1002/1097-4547(20010101)63:1<90::AID-JNR11>3.0.CO;2-9
dc.identifier.eissnpl
1097-4547
dc.identifier.issnpl
0360-4012
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/30925
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights.licence
bez licencji
dc.subtypepl
Article
dc.titlepl
Morphology of reactive microglia in the injured cerebral cortex : fractal analysis and complementary quantitative methods
dc.title.journalpl
Journal of Neuroscience Research
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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