Nitric oxide spin-trapping and NADPH-diaphorase activity in mature rat brain after injury

2007
journal article
article
cris.lastimport.scopus2024-04-07T17:43:30Z
cris.lastimport.wos2024-04-09T23:25:44Z
dc.abstract.otherTraumatic brain injury (TBI) induces inflammatory reactions, and one of the essential mediators of this reaction is nitric oxide (NO). The action of this compound is still under study because no clear consensus has been reached about its exact action in the central nervous system. Further, it is unknown if, in the damaged brain, its neuroprotective activity outweighs its putative neurodegenerative properties. Using ferrous-diethyldithiocarbamate chelate, a lipophilic spin trap for NO detection by electron paramagnetic resonance (EPR) spectroscopy, we followed NO production in injured brain of mature Wistar rats. To relate changes in the amount of NO in the lesioned brain to the activity of NO synthase (NOS), this study also used NADPH-diaphorase staining. Our data show a rapid drop of NO concentration in the damaged brain below control values. This phenomenon persisted over several hours postinjury and varied with brain region. This decrease in NO concentration was accompanied by a simultaneous increase in the number of NADPH-diaphorase-positive cells, perhaps indicative of increased NOS activity. It is therefore assumed that, in the lesioned brain, a very rapid removal of NO occurs via its transformation to other reactive species such as peroxynitrite, which further adversely influence the damaged tissue.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizykipl
dc.affiliationWydział Farmaceutyczny : Zakład Chemii Nieorganicznejpl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki Molekularnejpl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorZiaja, Marek - 132904 pl
dc.contributor.authorPyka, Janusz - 131594 pl
dc.contributor.authorMachowska, Annapl
dc.contributor.authorMaślanka, Anna - 130890 pl
dc.contributor.authorPłonka, Przemysław - 131459 pl
dc.date.accessioned2016-09-27T13:54:52Z
dc.date.available2016-09-27T13:54:52Z
dc.date.issued2007pl
dc.description.number12pl
dc.description.physical1845-1854pl
dc.description.volume24pl
dc.identifier.doi10.1089/neu.2007.0303pl
dc.identifier.eissn1557-9042pl
dc.identifier.issn0897-7151pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/30854
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subtypeArticlepl
dc.titleNitric oxide spin-trapping and NADPH-diaphorase activity in mature rat brain after injurypl
dc.title.journalJournal of Neurotraumapl
dc.typeJournalArticlepl
dspace.entity.typePublication
Affiliations

* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.

Views
0
Views per month

No access

No Thumbnail Available