Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary

2012
journal article
article
16
dc.abstract.enThe aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism. Basal CYP1A1 and CYP2B1/2 activity increased during culture. BDE-47 had no effect on CYP1A1, however increased CYP2B1/2 activity after exposure for 6 h. Basal SULT1A activity was 2.5 fold lower than that of COMT, and both proteins were stable during culture. BDE-47 increased SULT1A after exposure for 6 h, and COMT activity after exposure for 24 and 48 h. BDE-47 had no effect on the expression of all investigated enzymes. In conclusion, fast activation of CYP2B1/2 and late activation of COMT (with a very low basal SULT1A activity) indicates a possible action of locally produced hydroxylated metabolites prior to their detoxification.pl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorKarpeta, Anna - 104122 pl
dc.contributor.authorWarzecha, Katarzynapl
dc.contributor.authorJerzak, Justynapl
dc.contributor.authorPtak, Anna - 131580 pl
dc.contributor.authorGregoraszczuk, Ewa - 128170 pl
dc.date.accession2015-04-13pl
dc.date.accessioned2016-08-03T09:08:50Z
dc.date.available2016-08-03T09:08:50Z
dc.date.issued2012pl
dc.description.number3pl
dc.description.physical436-442pl
dc.description.volume34pl
dc.identifier.doi10.1016/j.reprotox.2012.06.009pl
dc.identifier.eissn1873-1708pl
dc.identifier.issn0890-6238pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/29353
dc.identifier.weblinkhttp://www.sciencedirect.com/science/article/pii/S0890623812002353pl
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subject.enBDE-47pl
dc.subject.enCYP1A1pl
dc.subject.enCYP2B1/2pl
dc.subject.enSULT1Apl
dc.subject.enCOMTpl
dc.subject.enovarypl
dc.subtypeArticlepl
dc.titleActivation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovarypl
dc.title.journalReproductive Toxicologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
The aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism. Basal CYP1A1 and CYP2B1/2 activity increased during culture. BDE-47 had no effect on CYP1A1, however increased CYP2B1/2 activity after exposure for 6 h. Basal SULT1A activity was 2.5 fold lower than that of COMT, and both proteins were stable during culture. BDE-47 increased SULT1A after exposure for 6 h, and COMT activity after exposure for 24 and 48 h. BDE-47 had no effect on the expression of all investigated enzymes. In conclusion, fast activation of CYP2B1/2 and late activation of COMT (with a very low basal SULT1A activity) indicates a possible action of locally produced hydroxylated metabolites prior to their detoxification.
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Karpeta, Anna - 104122
dc.contributor.authorpl
Warzecha, Katarzyna
dc.contributor.authorpl
Jerzak, Justyna
dc.contributor.authorpl
Ptak, Anna - 131580
dc.contributor.authorpl
Gregoraszczuk, Ewa - 128170
dc.date.accessionpl
2015-04-13
dc.date.accessioned
2016-08-03T09:08:50Z
dc.date.available
2016-08-03T09:08:50Z
dc.date.issuedpl
2012
dc.description.numberpl
3
dc.description.physicalpl
436-442
dc.description.volumepl
34
dc.identifier.doipl
10.1016/j.reprotox.2012.06.009
dc.identifier.eissnpl
1873-1708
dc.identifier.issnpl
0890-6238
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/29353
dc.identifier.weblinkpl
http://www.sciencedirect.com/science/article/pii/S0890623812002353
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
bez licencji
dc.rights.uri*
dc.subject.enpl
BDE-47
dc.subject.enpl
CYP1A1
dc.subject.enpl
CYP2B1/2
dc.subject.enpl
SULT1A
dc.subject.enpl
COMT
dc.subject.enpl
ovary
dc.subtypepl
Article
dc.titlepl
Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary
dc.title.journalpl
Reproductive Toxicology
dc.typepl
JournalArticle
dspace.entity.type
Publication

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