Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules

2021
journal article
article
3
cris.lastimport.wos2024-04-09T19:12:05Z
dc.abstract.enProtein content of extracellular vesicles (EVs) can modulate different processes during carcinogenesis. Novel proteomic strategies have been applied several times to profile proteins present in exosomes released by urothelial bladder cancer (UBC) cells. However, similar studies have not been conducted so far on another population of EVs, i.e., ectosomes. In the present study we used a shotgun nanoLC–MS/MS proteomic approach to investigate the protein content of ectosomes released in vitro by T-24 UBC cells and HCV-29 normal ureter epithelial cells. In addition, cancer-promoting effects exerted by UBC-derived ectosomes on non-invasive cells in terms of cell proliferation and migratory properties were assessed. In total, 1158 proteins were identified in T-24-derived ectosomes, while HCV-29-derived ectosomes contained a lower number of 259 identified proteins. Qualitative analysis revealed 938 proteins present uniquely in T-24-derived ectosomes, suggesting their potential applications in bladder cancer management as diagnostic and prognostic biomarkers. In addition, T-24-derived ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of the identified cancer-promoting molecules. The present study provided a focused identification of biologically relevant proteins in UBC-derived ectosomes, confirming their role in UBC development and progression, and their applicability for further biomarker-oriented studies in preclinical or clinical settings.pl
dc.affiliationWydział Biologii : Instytut Zoologii i Badań Biomedycznychpl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznejpl
dc.affiliationPion Prorektora ds. badań naukowych : Małopolskie Centrum Biotechnologiipl
dc.contributor.authorSurman, Magdalena - 192235 pl
dc.contributor.authorKędracka-Krok, Sylwia - 128739 pl
dc.contributor.authorJankowska, Urszula - 103969 pl
dc.contributor.authorDrożdż, Anna - 164492 pl
dc.contributor.authorStępień, Ewa - 161583 pl
dc.contributor.authorPrzybyło, Małgorzata - 131574 pl
dc.date.accessioned2021-07-12T16:53:45Z
dc.date.available2021-07-12T16:53:45Z
dc.date.issued2021pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number13pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume22pl
dc.identifier.articleid6816pl
dc.identifier.doi10.3390/ijms22136816pl
dc.identifier.eissn1422-0067pl
dc.identifier.issn1661-6596pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/276207
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enbiomarkerspl
dc.subject.enbladder cancerpl
dc.subject.enectosomespl
dc.subject.enextracellular vesiclespl
dc.subject.ennanoLC-MS/MSpl
dc.subject.enmass spectrometrypl
dc.subject.enproteomicspl
dc.subtypeArticlepl
dc.titleProteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant moleculespl
dc.title.journalInternational Journal of Molecular Sciencespl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T19:12:05Z
dc.abstract.enpl
Protein content of extracellular vesicles (EVs) can modulate different processes during carcinogenesis. Novel proteomic strategies have been applied several times to profile proteins present in exosomes released by urothelial bladder cancer (UBC) cells. However, similar studies have not been conducted so far on another population of EVs, i.e., ectosomes. In the present study we used a shotgun nanoLC–MS/MS proteomic approach to investigate the protein content of ectosomes released in vitro by T-24 UBC cells and HCV-29 normal ureter epithelial cells. In addition, cancer-promoting effects exerted by UBC-derived ectosomes on non-invasive cells in terms of cell proliferation and migratory properties were assessed. In total, 1158 proteins were identified in T-24-derived ectosomes, while HCV-29-derived ectosomes contained a lower number of 259 identified proteins. Qualitative analysis revealed 938 proteins present uniquely in T-24-derived ectosomes, suggesting their potential applications in bladder cancer management as diagnostic and prognostic biomarkers. In addition, T-24-derived ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of the identified cancer-promoting molecules. The present study provided a focused identification of biologically relevant proteins in UBC-derived ectosomes, confirming their role in UBC development and progression, and their applicability for further biomarker-oriented studies in preclinical or clinical settings.
dc.affiliationpl
Wydział Biologii : Instytut Zoologii i Badań Biomedycznych
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej
dc.affiliationpl
Pion Prorektora ds. badań naukowych : Małopolskie Centrum Biotechnologii
dc.contributor.authorpl
Surman, Magdalena - 192235
dc.contributor.authorpl
Kędracka-Krok, Sylwia - 128739
dc.contributor.authorpl
Jankowska, Urszula - 103969
dc.contributor.authorpl
Drożdż, Anna - 164492
dc.contributor.authorpl
Stępień, Ewa - 161583
dc.contributor.authorpl
Przybyło, Małgorzata - 131574
dc.date.accessioned
2021-07-12T16:53:45Z
dc.date.available
2021-07-12T16:53:45Z
dc.date.issuedpl
2021
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
13
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
22
dc.identifier.articleidpl
6816
dc.identifier.doipl
10.3390/ijms22136816
dc.identifier.eissnpl
1422-0067
dc.identifier.issnpl
1661-6596
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/276207
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
biomarkers
dc.subject.enpl
bladder cancer
dc.subject.enpl
ectosomes
dc.subject.enpl
extracellular vesicles
dc.subject.enpl
nanoLC-MS/MS
dc.subject.enpl
mass spectrometry
dc.subject.enpl
proteomics
dc.subtypepl
Article
dc.titlepl
Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules
dc.title.journalpl
International Journal of Molecular Sciences
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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