Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells

2014
journal article
article
6
dc.abstract.enObjectives: Properties of cell culture supports obtained from ultrathin multilayer films containing anionic natural polysaccharides (PSacs) and a synthetic polycation were studied. Materials and methods: Supports were prepared via a layer-by-layer (LbL) self-assembly deposition method. Polymers used were: heparin (Hep), chondroitin sulphate (CS), hyaluronic acid (HA), and ι-carrageenan (Car) as polyanions, and diazoresin (DR) as a polycation. PSac layers were crosslinked with DR layers by irradiation with UV light absorbed by DR resin. Results: DR/PSac films are very efficient cell culture growth supports as found from experiments with human mesenchymal stem cells (hMSCs). Irradiation of the films resulted in changing zeta potential of outermost layers of both DR and PSac to more negative values, and in increased film hydrophobicity, as found from the contact angle measurements. Photocrosslinking of the supports led to their increased stability. Conclusions: The supports allow for obtaining intact cell monolayers faster than when typical polystyrene tissue culture plates are used. Moreover, these monolayers spontaneously detach permitting formation of new cell layers on these surfaces relatively early during culture, compared to cells cultured on commonly used tissue culture plastic.pl
dc.affiliationWydział Chemii : Zakład Chemii Fizycznej i Elektrochemiipl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorMikulska, Annapl
dc.contributor.authorFilipowska, Joannapl
dc.contributor.authorOsyczka, Anna - 131214 pl
dc.contributor.authorSzuwarzyński, Michał - 126166 pl
dc.contributor.authorNowakowska, Maria - 131048 pl
dc.contributor.authorSzczubiałka, Krzysztof - 132218 pl
dc.date.accessioned2015-01-15T09:26:59Z
dc.date.available2015-01-15T09:26:59Z
dc.date.issued2014pl
dc.description.admin[AB] Mikulska, Anna 50000141pl
dc.description.number6pl
dc.description.physical516-526pl
dc.description.volume47pl
dc.identifier.doi10.1111/cpr.12118pl
dc.identifier.eissn1365-2184pl
dc.identifier.issn0960-7722pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/2550
dc.languageengpl
dc.language.containerengpl
dc.rights.licencebez licencji
dc.subtypeArticlepl
dc.titlePhotocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cellspl
dc.title.journalCell Proliferationpl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Objectives: Properties of cell culture supports obtained from ultrathin multilayer films containing anionic natural polysaccharides (PSacs) and a synthetic polycation were studied. Materials and methods: Supports were prepared via a layer-by-layer (LbL) self-assembly deposition method. Polymers used were: heparin (Hep), chondroitin sulphate (CS), hyaluronic acid (HA), and ι-carrageenan (Car) as polyanions, and diazoresin (DR) as a polycation. PSac layers were crosslinked with DR layers by irradiation with UV light absorbed by DR resin. Results: DR/PSac films are very efficient cell culture growth supports as found from experiments with human mesenchymal stem cells (hMSCs). Irradiation of the films resulted in changing zeta potential of outermost layers of both DR and PSac to more negative values, and in increased film hydrophobicity, as found from the contact angle measurements. Photocrosslinking of the supports led to their increased stability. Conclusions: The supports allow for obtaining intact cell monolayers faster than when typical polystyrene tissue culture plates are used. Moreover, these monolayers spontaneously detach permitting formation of new cell layers on these surfaces relatively early during culture, compared to cells cultured on commonly used tissue culture plastic.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Mikulska, Anna
dc.contributor.authorpl
Filipowska, Joanna
dc.contributor.authorpl
Osyczka, Anna - 131214
dc.contributor.authorpl
Szuwarzyński, Michał - 126166
dc.contributor.authorpl
Nowakowska, Maria - 131048
dc.contributor.authorpl
Szczubiałka, Krzysztof - 132218
dc.date.accessioned
2015-01-15T09:26:59Z
dc.date.available
2015-01-15T09:26:59Z
dc.date.issuedpl
2014
dc.description.adminpl
[AB] Mikulska, Anna 50000141
dc.description.numberpl
6
dc.description.physicalpl
516-526
dc.description.volumepl
47
dc.identifier.doipl
10.1111/cpr.12118
dc.identifier.eissnpl
1365-2184
dc.identifier.issnpl
0960-7722
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/2550
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights.licence
bez licencji
dc.subtypepl
Article
dc.titlepl
Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells
dc.title.journalpl
Cell Proliferation
dc.typepl
JournalArticle
dspace.entity.type
Publication

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