Application of micelle-mediated extraction in preparation of body fluids for HPLC-DAD screening of acidic and neutral drugs

2013
journal article
article
cris.lastimport.wos2024-04-10T01:40:27Z
dc.abstract.enExperimental conditions of cloud-point extraction (CPE) for the selected five acidic and neutral medicaments (salicylic acid, opipramol, carbamazepine, lorazepam, and alprazolam) in human plasma were studied and optimized. Separation and detection of the tested drugs were performed by the high-performance liquid chromatography with diode array detection (HPLC-DAD) method in an appropriate gradient mode using a column Nucleosil C8. Under the optimized conditions, main validation parameters were determined for all the compounds. The extraction yields (%) ranged from 54.12 to 82.17 with intra- and interday repeatability (RSD, %) 5.70-9.92 and 5.79-10.19, respectively. The detection limit was 0.5 $\mu$g mL$^{-1}$ for all the tested drugs with exception of salicylic acid (LOD = 2.5 $\mu$g mL$^{-1}$). The linearity of the proposed method was examined for the four drugs: opipramol, carbamazepine, lorazepam, and alprazolam in the concentration range of 0.5–2.0 $\mu$g mL$^{-1}$ (correlation coefficient r$^{2}$ = 0.995-0.999) and for salicylic acid in the concentration range of 2.5-10.0 $\mu$g mL$^{-1}$ (correlation coefficient r$^{2}$ = 0.993). The analytical parameters for the medicaments tested in whole blood were unsatisfactory, especially in terms of extraction recovery and repeatability, and application of the developed procedure for this biological matrix requires further study.pl
dc.affiliationWydział Chemii : Zakład Chemii Analitycznejpl
dc.contributor.authorMadej, Katarzyna - 130167 pl
dc.contributor.authorPersona, Karolina - 107636 pl
dc.contributor.authorNizio, Magdalenapl
dc.date.accession2023-10-13pl
dc.date.accessioned2014-12-20T10:47:27Z
dc.date.available2014-12-20T10:47:27Z
dc.date.issued2013pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.admin[AB] Nizio, Magdalena 50000141pl
dc.description.number1pl
dc.description.physical97-110pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume25pl
dc.identifier.doi10.1556/AChrom.25.2013.1.5pl
dc.identifier.eissn2083-5736pl
dc.identifier.issn1233-2356pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/2381
dc.identifier.weblinkhttps://akjournals.com/view/journals/1326/25/1/article-p97.xmlpl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne 4.0 Międzynarodowa*
dc.rights.licenceCC-BY-NC
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enmicelle-mediated extractionpl
dc.subject.encloud-point extractionpl
dc.subject.enscreening drug analysispl
dc.subject.enacidic/neutral medicamentspl
dc.subject.enbody fluidspl
dc.subtypeArticlepl
dc.titleApplication of micelle-mediated extraction in preparation of body fluids for HPLC-DAD screening of acidic and neutral drugspl
dc.title.journalActa Chromatographicapl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T01:40:27Z
dc.abstract.enpl
Experimental conditions of cloud-point extraction (CPE) for the selected five acidic and neutral medicaments (salicylic acid, opipramol, carbamazepine, lorazepam, and alprazolam) in human plasma were studied and optimized. Separation and detection of the tested drugs were performed by the high-performance liquid chromatography with diode array detection (HPLC-DAD) method in an appropriate gradient mode using a column Nucleosil C8. Under the optimized conditions, main validation parameters were determined for all the compounds. The extraction yields (%) ranged from 54.12 to 82.17 with intra- and interday repeatability (RSD, %) 5.70-9.92 and 5.79-10.19, respectively. The detection limit was 0.5 $\mu$g mL$^{-1}$ for all the tested drugs with exception of salicylic acid (LOD = 2.5 $\mu$g mL$^{-1}$). The linearity of the proposed method was examined for the four drugs: opipramol, carbamazepine, lorazepam, and alprazolam in the concentration range of 0.5–2.0 $\mu$g mL$^{-1}$ (correlation coefficient r$^{2}$ = 0.995-0.999) and for salicylic acid in the concentration range of 2.5-10.0 $\mu$g mL$^{-1}$ (correlation coefficient r$^{2}$ = 0.993). The analytical parameters for the medicaments tested in whole blood were unsatisfactory, especially in terms of extraction recovery and repeatability, and application of the developed procedure for this biological matrix requires further study.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Analitycznej
dc.contributor.authorpl
Madej, Katarzyna - 130167
dc.contributor.authorpl
Persona, Karolina - 107636
dc.contributor.authorpl
Nizio, Magdalena
dc.date.accessionpl
2023-10-13
dc.date.accessioned
2014-12-20T10:47:27Z
dc.date.available
2014-12-20T10:47:27Z
dc.date.issuedpl
2013
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.adminpl
[AB] Nizio, Magdalena 50000141
dc.description.numberpl
1
dc.description.physicalpl
97-110
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
25
dc.identifier.doipl
10.1556/AChrom.25.2013.1.5
dc.identifier.eissnpl
2083-5736
dc.identifier.issnpl
1233-2356
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/2381
dc.identifier.weblinkpl
https://akjournals.com/view/journals/1326/25/1/article-p97.xml
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne 4.0 Międzynarodowa
dc.rights.licence
CC-BY-NC
dc.rights.uri*
http://creativecommons.org/licenses/by-nc/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
micelle-mediated extraction
dc.subject.enpl
cloud-point extraction
dc.subject.enpl
screening drug analysis
dc.subject.enpl
acidic/neutral medicaments
dc.subject.enpl
body fluids
dc.subtypepl
Article
dc.titlepl
Application of micelle-mediated extraction in preparation of body fluids for HPLC-DAD screening of acidic and neutral drugs
dc.title.journalpl
Acta Chromatographica
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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