Verteporfin, photofrin II, and merocyanine 540 as PDT photosensitizers against melanoma cells

2006
journal article
article
56
cris.lastimport.wos2024-04-10T00:58:44Z
dc.abstract.enThe efficiency of photodynamic effect (PDE) for Photofrin II (PfII), Verteporfin, and Merocyanine 540 (MC540) was compared against neoplastic cells. Triplet state lifetimes and singlet molecular oxygen quantum yields were correlated with biological effect. PfII triplet lifetime was two times longer than that of Verteporfin, however, its singlet molecular oxygen quantum yield was two times lower in comparison with Verteporfin. High singlet molecular oxygen quantum yield of Verteporfin resulted in high biological efficacy. To achieve 50% mortality of cells four times lower light dose and five times lower concentration of Verteporfin were applied in comparison with PfII. The same level of cell damage was reached using 10 times higher light dose and two times higher concentration of MC540 in comparison with PfII. Our results confirm that singlet molecular oxygen based mechanism, prevalent for Verteporfin and PfII, was highly effective against melanoma cells. Verteporfin can be used at small doses with high cellular damage efficiency.pl
dc.affiliationWydział Chemii : Zakład Chemii Nieorganicznejpl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizykipl
dc.contributor.authorNowak-Sliwinska, Patrycjapl
dc.contributor.authorKarocki, Andrzejpl
dc.contributor.authorElas, Martyna - 127873 pl
dc.contributor.authorPawlak, Anna - 131304 pl
dc.contributor.authorStochel, Grażyna - 132108 pl
dc.contributor.authorUrbańska, Katarzynapl
dc.date.accessioned2014-12-18T08:16:07Z
dc.date.available2014-12-18T08:16:07Z
dc.date.issued2006pl
dc.description.number2pl
dc.description.physical549-555pl
dc.description.volume349pl
dc.identifier.doi10.1016/j.bbrc.2006.08.060pl
dc.identifier.eissn1090-2104pl
dc.identifier.issn0006-291Xpl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/2320
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subject.enphotodynamic effectpl
dc.subject.enphotofrin IIpl
dc.subject.enmerocyanine 540pl
dc.subject.enverteporfinpl
dc.subject.ensinglet molecular oxygenpl
dc.subject.entriplet lifetimepl
dc.subtypeArticlepl
dc.titleVerteporfin, photofrin II, and merocyanine 540 as PDT photosensitizers against melanoma cellspl
dc.title.journalBiochemical and Biophysical Research Communicationspl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T00:58:44Z
dc.abstract.enpl
The efficiency of photodynamic effect (PDE) for Photofrin II (PfII), Verteporfin, and Merocyanine 540 (MC540) was compared against neoplastic cells. Triplet state lifetimes and singlet molecular oxygen quantum yields were correlated with biological effect. PfII triplet lifetime was two times longer than that of Verteporfin, however, its singlet molecular oxygen quantum yield was two times lower in comparison with Verteporfin. High singlet molecular oxygen quantum yield of Verteporfin resulted in high biological efficacy. To achieve 50% mortality of cells four times lower light dose and five times lower concentration of Verteporfin were applied in comparison with PfII. The same level of cell damage was reached using 10 times higher light dose and two times higher concentration of MC540 in comparison with PfII. Our results confirm that singlet molecular oxygen based mechanism, prevalent for Verteporfin and PfII, was highly effective against melanoma cells. Verteporfin can be used at small doses with high cellular damage efficiency.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Nieorganicznej
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biofizyki
dc.contributor.authorpl
Nowak-Sliwinska, Patrycja
dc.contributor.authorpl
Karocki, Andrzej
dc.contributor.authorpl
Elas, Martyna - 127873
dc.contributor.authorpl
Pawlak, Anna - 131304
dc.contributor.authorpl
Stochel, Grażyna - 132108
dc.contributor.authorpl
Urbańska, Katarzyna
dc.date.accessioned
2014-12-18T08:16:07Z
dc.date.available
2014-12-18T08:16:07Z
dc.date.issuedpl
2006
dc.description.numberpl
2
dc.description.physicalpl
549-555
dc.description.volumepl
349
dc.identifier.doipl
10.1016/j.bbrc.2006.08.060
dc.identifier.eissnpl
1090-2104
dc.identifier.issnpl
0006-291X
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/2320
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
bez licencji
dc.rights.uri*
dc.subject.enpl
photodynamic effect
dc.subject.enpl
photofrin II
dc.subject.enpl
merocyanine 540
dc.subject.enpl
verteporfin
dc.subject.enpl
singlet molecular oxygen
dc.subject.enpl
triplet lifetime
dc.subtypepl
Article
dc.titlepl
Verteporfin, photofrin II, and merocyanine 540 as PDT photosensitizers against melanoma cells
dc.title.journalpl
Biochemical and Biophysical Research Communications
dc.typepl
JournalArticle
dspace.entity.type
Publication

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