Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy

2015
journal article
article
19
cris.lastimport.wos2024-04-10T03:26:57Z
dc.abstract.enResults of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.pl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Zakład Technologii Informatycznychpl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Zakład Technologii Gierpl
dc.affiliationWydział Chemii : Zakład Technologii Chemicznejpl
dc.contributor.authorKubicz, Ewelina - 177092 pl
dc.contributor.authorJasińska, Bożenapl
dc.contributor.authorZgardzińska, Bożenapl
dc.contributor.authorBednarski, Tomasz - 115868 pl
dc.contributor.authorBiałas, Piotr - 127296 pl
dc.contributor.authorCzerwiński, Eryk - 102850 pl
dc.contributor.authorGajos, Aleksander - 164087 pl
dc.contributor.authorGorgol, Marekpl
dc.contributor.authorKisielewska, Daria - 179828 pl
dc.contributor.authorKapłon, Łukasz - 115081 pl
dc.contributor.authorKochanowski, Andrzej - 128957 pl
dc.contributor.authorKorcyl, Grzegorz - 107362 pl
dc.contributor.authorKowalski, Pawełpl
dc.contributor.authorKozik, Tomasz - 129362 pl
dc.contributor.authorKrzemień, Wojciech - 135126 pl
dc.contributor.authorNiedźwiecki, Szymon - 115136 pl
dc.contributor.authorPałka, Marek - 214237 pl
dc.contributor.authorRaczyński, Lechpl
dc.contributor.authorRajfur, Zenon - 214965 pl
dc.contributor.authorRudy, Zbigniew - 100004 pl
dc.contributor.authorRundel, Oleksandr - 249228 pl
dc.contributor.authorGupta-Sharma, Neha - 235987 pl
dc.contributor.authorSilarski, Michał - 103624 pl
dc.contributor.authorSłomski, Artur - 109394 pl
dc.contributor.authorStrzelecki, Adam - 182201 pl
dc.contributor.authorWieczorek, Anna - 163447 pl
dc.contributor.authorWiślicki, Wojciechpl
dc.contributor.authorZieliński, Marcin - 124902 pl
dc.contributor.authorMoskal, Paweł - 100401 pl
dc.date.accession2016-03-17pl
dc.date.accessioned2016-03-17T12:56:37Z
dc.date.available2016-03-17T12:56:37Z
dc.date.issued2015pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalAutorka Daria Kisielewska podpisana jako Daria Kamińska.pl
dc.description.number4pl
dc.description.physical749-753pl
dc.description.publication0,3pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume60pl
dc.identifier.doi10.1515/nuka-2015-0135pl
dc.identifier.eissn1508-5791pl
dc.identifier.issn0029-5922pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/22550
dc.identifier.weblinkhttp://www.nukleonika.pl/www/back/full/vol60_2015/v60n4p749f.pdfpl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska*
dc.rights.licenceCC-BY-NC-ND
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/legalcode*
dc.share.typeotwarte czasopismo
dc.subject.enenvironmental scanning electron microscopy (ESEM)pl
dc.subject.enfree volumepl
dc.subject.eninverted microscopypl
dc.subject.enpositron annihilationpl
dc.subject.enpositroniumpl
dc.subject.enyeastspl
dc.subtypeArticlepl
dc.titleStudies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopypl
dc.title.journalNukleonikapl
dc.title.volumeProceedings of the 42nd Polish Seminar on Positron Annihilation Lublin, Poland, 29 June – 1 July 2015pl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T03:26:57Z
dc.abstract.enpl
Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Zakład Technologii Informatycznych
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Zakład Technologii Gier
dc.affiliationpl
Wydział Chemii : Zakład Technologii Chemicznej
dc.contributor.authorpl
Kubicz, Ewelina - 177092
dc.contributor.authorpl
Jasińska, Bożena
dc.contributor.authorpl
Zgardzińska, Bożena
dc.contributor.authorpl
Bednarski, Tomasz - 115868
dc.contributor.authorpl
Białas, Piotr - 127296
dc.contributor.authorpl
Czerwiński, Eryk - 102850
dc.contributor.authorpl
Gajos, Aleksander - 164087
dc.contributor.authorpl
Gorgol, Marek
dc.contributor.authorpl
Kisielewska, Daria - 179828
dc.contributor.authorpl
Kapłon, Łukasz - 115081
dc.contributor.authorpl
Kochanowski, Andrzej - 128957
dc.contributor.authorpl
Korcyl, Grzegorz - 107362
dc.contributor.authorpl
Kowalski, Paweł
dc.contributor.authorpl
Kozik, Tomasz - 129362
dc.contributor.authorpl
Krzemień, Wojciech - 135126
dc.contributor.authorpl
Niedźwiecki, Szymon - 115136
dc.contributor.authorpl
Pałka, Marek - 214237
dc.contributor.authorpl
Raczyński, Lech
dc.contributor.authorpl
Rajfur, Zenon - 214965
dc.contributor.authorpl
Rudy, Zbigniew - 100004
dc.contributor.authorpl
Rundel, Oleksandr - 249228
dc.contributor.authorpl
Gupta-Sharma, Neha - 235987
dc.contributor.authorpl
Silarski, Michał - 103624
dc.contributor.authorpl
Słomski, Artur - 109394
dc.contributor.authorpl
Strzelecki, Adam - 182201
dc.contributor.authorpl
Wieczorek, Anna - 163447
dc.contributor.authorpl
Wiślicki, Wojciech
dc.contributor.authorpl
Zieliński, Marcin - 124902
dc.contributor.authorpl
Moskal, Paweł - 100401
dc.date.accessionpl
2016-03-17
dc.date.accessioned
2016-03-17T12:56:37Z
dc.date.available
2016-03-17T12:56:37Z
dc.date.issuedpl
2015
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Autorka Daria Kisielewska podpisana jako Daria Kamińska.
dc.description.numberpl
4
dc.description.physicalpl
749-753
dc.description.publicationpl
0,3
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
60
dc.identifier.doipl
10.1515/nuka-2015-0135
dc.identifier.eissnpl
1508-5791
dc.identifier.issnpl
0029-5922
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/22550
dc.identifier.weblinkpl
http://www.nukleonika.pl/www/back/full/vol60_2015/v60n4p749f.pdf
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska
dc.rights.licence
CC-BY-NC-ND
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode
dc.share.type
otwarte czasopismo
dc.subject.enpl
environmental scanning electron microscopy (ESEM)
dc.subject.enpl
free volume
dc.subject.enpl
inverted microscopy
dc.subject.enpl
positron annihilation
dc.subject.enpl
positronium
dc.subject.enpl
yeasts
dc.subtypepl
Article
dc.titlepl
Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy
dc.title.journalpl
Nukleonika
dc.title.volumepl
Proceedings of the 42nd Polish Seminar on Positron Annihilation Lublin, Poland, 29 June – 1 July 2015
dc.typepl
JournalArticle
dspace.entity.type
Publication

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