Effect of hydrogen peroxide of bacterial origin on apoptosis and necrosis of gut mucosa epithelial cells as a possible pathomechanism of inflammatory bowel disease and cancer

2009
journal article
article
dc.abstract.enA series of in vitro experiments was arranged to assess effects of different concentrations of $H_{2}O_{2}$ contained in bacterial cultures on apoptosis and necrosis of HT-29 line cells representing human gut epithelium. On the basis of cytofluorimetric assays it was possible to demonstrate that supernatant of the Lactobacillus strain producing hydrogen peroxide (L. delbrueckii CU/22) was able to induce both apoptosis and necrosis in human epithelial culture cells HT-29. Both effects were more prominent than those visible under influence of supernatant of the non-$H_{2}O_{2}$-producing Lactobacillus strain or chemically pure $H_{2}O_{2}$ at the same concentration used as a control. In the light of this study and also other reports on damaging effects of hydrogen peroxide and superoxide radicals of bacterial origin on colonic cells, commensal bacteria of the human gut producing $H_{2}O_{2}$ may be involved in pathomechanisms of IBD by perpetuating the inflammatory reaction and increasing apoptosis and necrosis. There is a promise that probiotic preparations containing Lactobacillus bacteria will be successful as adjunct therapy of IBD and it is, therefore, postulated to make a very careful selection of the Lactobacillus strains as candidates for probiotics indicated to ameliorate the course of IBD, before starting clinical trials.pl
dc.affiliationWydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologiipl
dc.affiliationWydział Chemii : Zakład Chemii Nieorganicznejpl
dc.contributor.authorStrus, Magdalena - 133523 pl
dc.contributor.authorKyzioł, Agnieszka - 147927 pl
dc.contributor.authorGonet-Surówka, Agnieszka - 162000 pl
dc.contributor.authorBrzychczy-Włoch, Monika - 128897 pl
dc.contributor.authorStochel, Grażyna - 132108 pl
dc.contributor.authorKochan, Piotr - 130192 pl
dc.contributor.authorHeczko, Piotr - 129700 pl
dc.date.accession2015-09-01pl
dc.date.accessioned2015-09-14T10:41:29Z
dc.date.available2015-09-14T10:41:29Z
dc.date.issued2009pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalNa publikacji autorka Kyzioł Agnieszka podpisana jako Jańczyk Agnieszka.pl
dc.description.numbersuppl. 6pl
dc.description.physical55-60pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume60pl
dc.identifier.eissn1899-1505pl
dc.identifier.issn0867-5910pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/15623
dc.identifier.weblinkhttp://www.jpp.krakow.pl/journal/archive/12_09_s6/pdf/55_12_09_s6_article.pdfpl
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceOTHER
dc.rights.uri*
dc.share.typeotwarte czasopismo
dc.subtypeArticlepl
dc.titleEffect of hydrogen peroxide of bacterial origin on apoptosis and necrosis of gut mucosa epithelial cells as a possible pathomechanism of inflammatory bowel disease and cancerpl
dc.title.journalJournal of Physiology and Pharmacologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
A series of in vitro experiments was arranged to assess effects of different concentrations of $H_{2}O_{2}$ contained in bacterial cultures on apoptosis and necrosis of HT-29 line cells representing human gut epithelium. On the basis of cytofluorimetric assays it was possible to demonstrate that supernatant of the Lactobacillus strain producing hydrogen peroxide (L. delbrueckii CU/22) was able to induce both apoptosis and necrosis in human epithelial culture cells HT-29. Both effects were more prominent than those visible under influence of supernatant of the non-$H_{2}O_{2}$-producing Lactobacillus strain or chemically pure $H_{2}O_{2}$ at the same concentration used as a control. In the light of this study and also other reports on damaging effects of hydrogen peroxide and superoxide radicals of bacterial origin on colonic cells, commensal bacteria of the human gut producing $H_{2}O_{2}$ may be involved in pathomechanisms of IBD by perpetuating the inflammatory reaction and increasing apoptosis and necrosis. There is a promise that probiotic preparations containing Lactobacillus bacteria will be successful as adjunct therapy of IBD and it is, therefore, postulated to make a very careful selection of the Lactobacillus strains as candidates for probiotics indicated to ameliorate the course of IBD, before starting clinical trials.
dc.affiliationpl
Wydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologii
dc.affiliationpl
Wydział Chemii : Zakład Chemii Nieorganicznej
dc.contributor.authorpl
Strus, Magdalena - 133523
dc.contributor.authorpl
Kyzioł, Agnieszka - 147927
dc.contributor.authorpl
Gonet-Surówka, Agnieszka - 162000
dc.contributor.authorpl
Brzychczy-Włoch, Monika - 128897
dc.contributor.authorpl
Stochel, Grażyna - 132108
dc.contributor.authorpl
Kochan, Piotr - 130192
dc.contributor.authorpl
Heczko, Piotr - 129700
dc.date.accessionpl
2015-09-01
dc.date.accessioned
2015-09-14T10:41:29Z
dc.date.available
2015-09-14T10:41:29Z
dc.date.issuedpl
2009
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Na publikacji autorka Kyzioł Agnieszka podpisana jako Jańczyk Agnieszka.
dc.description.numberpl
suppl. 6
dc.description.physicalpl
55-60
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
60
dc.identifier.eissnpl
1899-1505
dc.identifier.issnpl
0867-5910
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/15623
dc.identifier.weblinkpl
http://www.jpp.krakow.pl/journal/archive/12_09_s6/pdf/55_12_09_s6_article.pdf
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
OTHER
dc.rights.uri*
dc.share.type
otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
Effect of hydrogen peroxide of bacterial origin on apoptosis and necrosis of gut mucosa epithelial cells as a possible pathomechanism of inflammatory bowel disease and cancer
dc.title.journalpl
Journal of Physiology and Pharmacology
dc.typepl
JournalArticle
dspace.entity.type
Publication

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