Interactions of a hydrophobically modified polycation with zwitterionic lipid membranes

2012
journal article
article
40
cris.lastimport.wos2024-04-10T03:22:47Z
dc.abstract.enThe interactions between synthetic polycations and phospho- lipid bilayers play an important role in some biophysical applications such as gene delivery or antibacterial usage. Despite extensive investigation into the nature of these interactions, their physical and molecular bases remain poorly understood. In this Article, we present the results ofour studies on the impact of a hydrophobically modi fi ed strong polycation on the properties of a zwitter- ionic bilayer used as a model of the mammalian cellular membrane. The study was carried out using a set of complementary experimental methods and molecular dynamic (MD) simulations. A new polycation, poly(allyl- N , N - dimethyl- N -hexylammonium chloride) (polymer 3 ), was synthesized, and its interactions with liposomes composed of 2-oleoyl-1-palmitoyl- sn -glycero-3- phosphocholine (POPC) were examined using dynamic light scattering (DLS), zeta potential measurements, and cryo-transmission electron microscopy (cryo-TEM). Our results have shown that polymer 3 can e ffi ciently associate with and insert into the POPC membrane. However, it does not change its lamellar structure, as was demonstrated by cryo-TEM. The in fl uence of polymer 3 on the membrane functionality was studied by leakage experiments applying a fl uorescence dye (calcein) encapsulated in the phospholipid vesicles. The MD simulations of model systems reveal that polymer 3 promotes formation of hydrophilic pores in the membrane, thus increasing considerably its permeability.pl
dc.affiliationWydział Chemii : Zakład Chemii Fizycznej i Elektrochemiipl
dc.contributor.authorKępczyński, Mariusz - 128750 pl
dc.contributor.authorJamróz, Dorota - 128440 pl
dc.contributor.authorWytrwał, Magdalena - 126175 pl
dc.contributor.authorBednar, Janpl
dc.contributor.authorRząd, Ewa - 107440 pl
dc.contributor.authorNowakowska, Maria - 131048 pl
dc.date.accessioned2015-09-02T12:41:01Z
dc.date.available2015-09-02T12:41:01Z
dc.date.issued2012pl
dc.description.number1pl
dc.description.physical676-688pl
dc.description.points35pl
dc.description.volume28pl
dc.identifier.doi10.1021/la203748qpl
dc.identifier.eissn1520-5827pl
dc.identifier.issn0743-7463pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/15298
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subtypeArticlepl
dc.titleInteractions of a hydrophobically modified polycation with zwitterionic lipid membranespl
dc.title.journalLangmuirpl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-10T03:22:47Z
dc.abstract.enpl
The interactions between synthetic polycations and phospho- lipid bilayers play an important role in some biophysical applications such as gene delivery or antibacterial usage. Despite extensive investigation into the nature of these interactions, their physical and molecular bases remain poorly understood. In this Article, we present the results ofour studies on the impact of a hydrophobically modi fi ed strong polycation on the properties of a zwitter- ionic bilayer used as a model of the mammalian cellular membrane. The study was carried out using a set of complementary experimental methods and molecular dynamic (MD) simulations. A new polycation, poly(allyl- N , N - dimethyl- N -hexylammonium chloride) (polymer 3 ), was synthesized, and its interactions with liposomes composed of 2-oleoyl-1-palmitoyl- sn -glycero-3- phosphocholine (POPC) were examined using dynamic light scattering (DLS), zeta potential measurements, and cryo-transmission electron microscopy (cryo-TEM). Our results have shown that polymer 3 can e ffi ciently associate with and insert into the POPC membrane. However, it does not change its lamellar structure, as was demonstrated by cryo-TEM. The in fl uence of polymer 3 on the membrane functionality was studied by leakage experiments applying a fl uorescence dye (calcein) encapsulated in the phospholipid vesicles. The MD simulations of model systems reveal that polymer 3 promotes formation of hydrophilic pores in the membrane, thus increasing considerably its permeability.
dc.affiliationpl
Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii
dc.contributor.authorpl
Kępczyński, Mariusz - 128750
dc.contributor.authorpl
Jamróz, Dorota - 128440
dc.contributor.authorpl
Wytrwał, Magdalena - 126175
dc.contributor.authorpl
Bednar, Jan
dc.contributor.authorpl
Rząd, Ewa - 107440
dc.contributor.authorpl
Nowakowska, Maria - 131048
dc.date.accessioned
2015-09-02T12:41:01Z
dc.date.available
2015-09-02T12:41:01Z
dc.date.issuedpl
2012
dc.description.numberpl
1
dc.description.physicalpl
676-688
dc.description.pointspl
35
dc.description.volumepl
28
dc.identifier.doipl
10.1021/la203748q
dc.identifier.eissnpl
1520-5827
dc.identifier.issnpl
0743-7463
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/15298
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
bez licencji
dc.rights.uri*
dc.subtypepl
Article
dc.titlepl
Interactions of a hydrophobically modified polycation with zwitterionic lipid membranes
dc.title.journalpl
Langmuir
dc.typepl
JournalArticle
dspace.entity.type
Publication

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