Interaction of apo-transferrin with anticancer ruthenium complexes NAMI-A and its reduced form

2012
journal article
article
dc.abstract.enNAMI-Ai.e. $(ImH)[trans-RuCl_{4}(DMSO)(Im)]$ (whereImisimidazole) is aruthenium(III) complex withpromising antimetastatic activity, which has been classified for II phase clinical trial. In this study, its binding properties to- wardapo-transferrin(apo-Tf) with regard to its hydrolytic and redox behavior are systematically investigated by the use of fluorescence spectroscopy. The reaction of NAMI-A and its reduced form with apo-Tf is proceeded by formation of aqua derivativesand thepresenceofat least onelabileaqua ligand issufficient to form adducts. It is found that presence of bicarbonate is not necessary for interaction of studied ruthenium complexes with apo-Tf. The calculated association constants for both NAMI-A and its reduced form are very similar with the values of $1.28x10^{4}M^{-1}$ and $1.36x10^{4}M^{-1}$ at $37^{o}\textrm{C}$, respectively however, the reduced derivatives reach the equilib- rium ca. 8-10 times slower. The percentage of ruthenium content in protein fractions separated from protein-unbounded ruthenium by using FPLC (fast protein liquid chromatography) method is rather high and depends on redox state of the complex, for most samples is found higher for reduced speciespl
dc.affiliationWydział Chemii : Zakład Chemii Nieorganicznejpl
dc.affiliationWydział Chemii : Zakład Krystalochemii i Krystalofizykipl
dc.contributor.authorMazuryk, Olga - 124952 pl
dc.contributor.authorKurpiewska, Katarzyna - 142859 pl
dc.contributor.authorLewiński, Krzysztof - 129948 pl
dc.contributor.authorStochel, Grażyna - 132108 pl
dc.contributor.authorBrindell, Małgorzata - 127426 pl
dc.date.accessioned2015-08-21T11:27:46Z
dc.date.available2015-08-21T11:27:46Z
dc.date.issued2012pl
dc.description.additionalBibliogr. s. 18pl
dc.description.physical11-18pl
dc.description.points35pl
dc.description.volume116pl
dc.identifier.doi10.1016/j.jinorgbio.2012.07.017pl
dc.identifier.eissn1873-3344pl
dc.identifier.issn0162-0134pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/14825
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licencebez licencji
dc.rights.uri*
dc.subject.enNAMI-Apl
dc.subject.enapo-transferrinpl
dc.subject.enassociation constantpl
dc.subject.enfluorescence quenchingpl
dc.subject.enseparation on FPLCpl
dc.subject.enruthenium contentpl
dc.subtypeArticlepl
dc.titleInteraction of apo-transferrin with anticancer ruthenium complexes NAMI-A and its reduced formpl
dc.title.journalJournal of Inorganic Biochemistrypl
dc.typeJournalArticlepl
dspace.entity.typePublication

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