International external quality assurance of "JAK2 V617F" quantification

2019
journal article
article
cris.lastimport.wos2024-04-09T20:08:40Z
dc.abstract.enExternal quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for JAK2 V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing JAK2 V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay set-up and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of JAK2 V617F allelic burden.pl
dc.affiliationWydział Lekarskipl
dc.cm.date2020-01-07
dc.cm.id94562
dc.contributor.authorAsp, Juliapl
dc.contributor.authorSkov, Vibepl
dc.contributor.authorBellosillo, Beatrizpl
dc.contributor.authorKristensen, Thomaspl
dc.contributor.authorLippert, Ericpl
dc.contributor.authorDicker, Frankpl
dc.contributor.authorSchwarz, Jiripl
dc.contributor.authorWojtaszewska, Marzenapl
dc.contributor.authorAkiki, Susannapl
dc.contributor.authorAggerholm, Annipl
dc.contributor.authorAndersen, Morten Tolstruppl
dc.contributor.authorGirodon, Francoispl
dc.contributor.authorKjaer, Lassepl
dc.contributor.authorOppliger Leibundgut, Elisabethpl
dc.contributor.authorPancrazzi, Alessandropl
dc.contributor.authorVorland, Martapl
dc.contributor.authorAndrikovics, Hajnalkapl
dc.contributor.authorKralovics, Robertpl
dc.contributor.authorCassinat, Brunopl
dc.contributor.authorCoucelo, Margaridapl
dc.contributor.authorEftimov, Aleksandarpl
dc.contributor.authorHaslam, Karlpl
dc.contributor.authorKusec, Rajkopl
dc.contributor.authorLink-Lenczowska, Dorotapl
dc.contributor.authorLode, Laurencepl
dc.contributor.authorMatiakowska, Karolinapl
dc.contributor.authorNaguib, Dinapl
dc.contributor.authorNavaglia, Filippopl
dc.contributor.authorNovotny, Guy Waynepl
dc.contributor.authorPercy, Melanie J.pl
dc.contributor.authorSudarikov, Andreypl
dc.contributor.authorHermouet, Sylviepl
dc.contributor.authorPallisgaard, Nielspl
dc.date.accessioned2020-01-17T10:09:14Z
dc.date.available2020-01-17T10:09:14Z
dc.date.issued2019pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number5pl
dc.description.physical1111-1118pl
dc.description.points70pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume98pl
dc.identifier.doi10.1007/s00277-018-3570-8pl
dc.identifier.eissn1432-0584pl
dc.identifier.issn0939-5555pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/146317
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeinne
dc.subject.enJAK2 V617Fpl
dc.subject.enexternal quality assurancepl
dc.subject.enmyeloproliferative neoplasmspl
dc.subject.enquantitative PCRpl
dc.subtypeArticlepl
dc.titleInternational external quality assurance of "JAK2 V617F" quantificationpl
dc.title.journalAnnals of Hematologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
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